OBJECTIVES: Concentrations of neopterin, kynurenine and kynurenine/tryptophan ratios predict prognosis and the need for oxygen therapy in patients hospitalized for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The aims of the present study were to evaluate the changes of these biomarkers early in the course of infection, the association with the prior coronavirus disease (COVID-19) vaccination and therapeutic administration of Anti-SARS-CoV-2 monoclonal antibodies, investigation of other potential biomarkers including neuropilin, 8-hydroxy-2-deoxyguanosine and 8-hydroxyguanosine in patients hospitalized with SARS-CoV-2 infection and an assessment of these biomarkers and vitamins A, E and D in patients with post-COVID syndrome. METHODS: Urine and blood samples were obtained on the 1st to the 4th day and 4th to 7th day from 108 patients hospitalized with COVID-19. Chromatography tandem mass spectrometry methods were used to analyse neopterin, kynurenine, tryptophan, liposoluble vitamins, and DNA damage biomarkers. RESULTS: A statistically significant decrease of neopterin, kynurenine and kynurenine/tryptophan ratios was observed on after 4th to 7th day of hospitalization, and concentrations of these biomarkers were increased in patients with poor prognosis and subsequent post-COVID syndrome. The concentrations of remaining biomarker and vitamins were not associated with outcomes, although markedly decreased concentrations of vitamin A, E and D were noted. CONCLUSIONS: The concentrations of neopterin, kynurenine and kynurenine/tryptophan ratios decrease during the course of infection SARS-CoV-2 and are associated with the post-COVID syndrome. No other prognostic biomarkers were identified.

• MeSH
• biologické markery * krev   MeSH
• COVID-19 * krev   MeSH
• dospělí MeSH
• hospitalizace MeSH
• kynurenin * krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• neopterin * krev   moč   MeSH
• postakutní syndrom COVID-19 MeSH
• SARS-CoV-2 * izolace a purifikace   MeSH
• senioři MeSH
• tryptofan * krev   MeSH
• vitamin A krev   MeSH
• vitamin D krev   MeSH
• vitamin E krev   MeSH
• vitaminy krev   MeSH
• zánět krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

• MeSH
• balneologie metody   trendy   MeSH
• duševní poruchy * metabolismus   rehabilitace   MeSH
• hydrokortison krev   metabolismus   MeSH
• kortikosteron krev   metabolismus   MeSH
• lidé středního věku MeSH
• lidé MeSH
• limbický systém metabolismus   MeSH
• plynová chromatografie s hmotnostně spektrometrickou detekcí metody   MeSH
• pregnenolon krev   metabolismus   MeSH
• sebeposuzující dotazníky PHQ MeSH
• serotonin krev   metabolismus   MeSH
• techniky cvičení a pohybu MeSH
• terapie cvičením MeSH
• tryptofan krev   metabolismus   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH

Among malignant diseases, chronic myeloid leukaemia (CML) is one of the best suited candidates for immunotherapy. For this purpose it is necessary to broaden the present knowledge on the immunology of this disease. As a part of such a project, the levels of kynurenine (KYN) and neopterin (NPT) were studied in 28 CML patients and in the same number of healthy subjects. At diagnosis, both KYN and NPT levels were found to be elevated in a significant portion of the patients and dependent on their leukocyte count. As in the case of KYN, increased NPT levels dropped after achieving remission. When correlating KYN and NPT levels with a selection of other markers tested, significant association was revealed only in the case of CRP and IL-6. However, there were several patients with increased KYN levels in whom NPT was not detected, and vice versa. The relapse of the disease observed in two patients was accompanied by an increased level of NPT in both cases, but by an increased level of KYN in only one of them. No significant correlation was found between KYN and NPT levels in sera taken at diagnosis. However, when the whole set of sera was taken into consideration, the association became statistically significant. Although the data obtained revealed a number of similarities between KYN and NPT production in CML patients, it also suggested a difference in the kinetics of these two biomarkers' production.

• MeSH
• biologické markery krev   MeSH
• C-reaktivní protein metabolismus   MeSH
• chronická myeloidní leukemie krev   MeSH
• dospělí MeSH
• interleukin-6 krev   MeSH
• kynurenin krev   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lineární modely MeSH
• mladý dospělý MeSH
• neopterin krev   MeSH
• počet leukocytů MeSH
• senioři MeSH
• studie případů a kontrol MeSH
• tryptofan krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH

Many compounds related to L-tryptophan (L-TRP) have interesting biological or pharmacological activity, and their abnormal neurotransmission seems to be linked to a wide range of neurodegenerative and psychiatric diseases. A high-throughput method based on ultra-high performance liquid chromatography connected to electrospray tandem mass spectrometry (UHPLC-ESI-MS/MS) was developed for the quantitative analysis of L-TRP and 16 of its metabolites in human serum and cerebrospinal fluid (CSF), representing both major and minor routes of L-TRP catabolism. The combination of a fast LC gradient with selective tandem mass spectrometry enabled accurate analysis of almost 100 samples in 24h. The standard isotope dilution method was used for quantitative determination. The method's lower limits of quantification for serum and cerebrospinal fluid ranged from 0.05 to 15nmol/L and 0.3 to 45nmol/L, respectively. Analytical recoveries ranged from 10.4 to 218.1% for serum and 22.1 to 370.0% for CSF. The method's accuracy ranged from 82.4 to 128.5% for serum matrix and 90.7 to 127.7% for CSF matrix. All intra- and inter-day coefficients of variation were below 15%. These results demonstrate that the new method is capable of quantifying endogenous serum and CSF levels of a heterogeneous group of compounds spanning a wide range of concentrations. The method was used to determine the physiological levels of target analytes in serum and CSF samples from 18 individuals, demonstrating its reliability and potential usefulness in large-scale epidemiological studies.

• MeSH
• biochemická analýza krve metody   MeSH
• chemické techniky analytické metody   MeSH
• indikátorové diluční techniky MeSH
• izotopy MeSH
• lidé MeSH
• reprodukovatelnost výsledků MeSH
• tandemová hmotnostní spektrometrie * MeSH
• tryptofan krev   MeSH
• vysokoúčinná kapalinová chromatografie * MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Indoleamine 2,3-dioxygenase 1 (IDO1), IDO2 and tryptophan 2,3-dioxygenase (TDO) represent some of the key immune regulators. Their increased activity has been demonstrated in a number of human malignancies but not yet in chronic myeloid leukemia (CML). In the present study, the activity of these enzymes was tested in 29 CML patients and 28 healthy subjects by monitoring the kynurenine (KYN)/tryptophan ratio. Serum samples taken prior to the therapy displayed a highly significant difference in KYN levels between the patient and control groups. However, increased KYN levels were detected in only 13 (44.8%) of these CML patients. The KYN levels in pretreatment sera of the patients correlated with the tumor burden. There was also a strong correlation between KYN levels and uric acid levels (UA). This suggests but does not prove the possible involvement of UA in activating IDO family of enzymes. Whenever tested, the increased KYN levels normalized in the course of the therapy. Patients with normal KYN levels in their pretreatment sera and subsequently treated with interferon-α, showed a transitory increase in their KYN levels. The present data indicate that CML should be added to the malignancies with an increased activity of the IDO family of enzymes and suggest that IDO inhibitors may be used in the treatment of CML patients.

• MeSH
• biologické markery krev   MeSH
• chronická myeloidní leukemie * MeSH
• dospělí MeSH
• indolamin-2,3,-dioxygenasa MeSH
• kynurenin * krev   MeSH
• kyselina močová * MeSH
• lidé středního věku MeSH
• lidé MeSH
• senioři MeSH
• tryptofan * krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH

A new HPLC method for simultaneous determination of neopterin, creatinine, kynurenine and tryptophan in human serum was developed and validated. Monolithic stationary phase's technology (two monolithic columns RP-18e were connected with guard monolithic cartridge 4.6 mm × 50 mm+3.0 mm × 100 mm and 4.6 × 10 mm) and special auto sampler for micro titration plates (samples are storage in dark cooled place protected against evaporation) were combined with easy sample preparation step. As mobile phase 15 mmol/L phosphate buffer at pH 4.50 was used. Neopterin and tryptophan were detected using fluorescent detection and kynurenine and creatinine were detected by diode-array detection. This method may be suitable for large sequences of samples in clinical research and routine practice.

• MeSH
• fosfáty chemie   MeSH
• koncentrace vodíkových iontů MeSH
• kreatinin krev   MeSH
• kynurenin krev   MeSH
• lidé MeSH
• neopterin krev   MeSH
• pufry MeSH
• reprodukovatelnost výsledků MeSH
• tryptofan krev   MeSH
• vysokoúčinná kapalinová chromatografie přístrojové vybavení   metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The goal of the present study was to investigate the daily amount of motor activity and the daily rhythm of tryptophan and serotonin in goats housed in individual boxes under a 12/12 light/dark cycle. We equipped six Girgentana breed goats with Actiwatch-Mini® (Cambridge Neurotechnology Ltd, UK), actigraphy-based data loggers that record a digitally integrated measure of motor activity. Also blood samples were collected at four-hour intervals over a 48 h period via an intravenous cannula inserted into the jugular vein. The concentration of tryptophan (TRP) and serotonin (5-HT) were assessed by the high-performance liquid chromatography (HPLC) method. Our results outline a diurnal pattern of motor activity and levels of tryptophan in the serum, and a nocturnal pattern of levels of serotonin in the serum, which underline the impact of endogenous serotoninergic activity on the behavioural aspects of the circadian response to light.

• MeSH
• cirkadiánní rytmus fyziologie   MeSH
• interpretace statistických dat MeSH
• kozy krev   MeSH
• pohybová aktivita fyziologie   účinky léků   MeSH
• serotonin krev   MeSH
• tryptofan krev   MeSH
• vysokoúčinná kapalinová chromatografie metody   využití   MeSH

• MeSH
• centrální nervový systém fyziologie   MeSH
• deprese MeSH
• krysa rodu rattus MeSH
• lidé MeSH
• modely nemocí na zvířatech MeSH
• nápoje MeSH
• poruchy nálady MeSH
• serotonin metabolismus   MeSH
• tryptofan krev   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• přehledy MeSH
• srovnávací studie MeSH

• MeSH
• tryptofan analýza   krev   moč   MeSH
• vysokoúčinná kapalinová chromatografie MeSH

• MeSH
• chronické selhání ledvin metabolismus   MeSH
• dialýza ledvin MeSH
• tryptofan krev   MeSH