Muscular sarcosporidial infections by Sarcocystis lutrae (Apicomplexa: Sarcocystidae) from the otter (Lutra lutra) and badger (Meles meles) (Carnivora: Mustelidae) were found in the Czech Republic. As part of a diversity evaluation of Sarcocystis in wild carnivores during 2016-2017, samples of diaphragm, tongue and hind-limb muscles were collected from nine districts, examined by compression and characterized molecularly. Cyst walls were thin, with no visible protrusions, and histological sections of infected muscle tissue showed no host responses. Fourteen of 17 badgers (82% prevalence) and one otter (100% prevalence) were positive for sarcocysts. Sequence analyses at four loci (18S rRNA, 28S rRNA, ITS1 and cox1) confirmed the identity as S. lutrae. This is also the first report of a co-infection with muscular sarcocystosis and Trichinella in badger. The finding of Trichinella is important from the zoonotic point of view, since badgers are used for meat consumption. Similar and future monitoring of both parasitic taxa are needed.
- MeSH
- Carnivora parazitologie MeSH
- koinfekce parazitologie veterinární MeSH
- molekulární typizace MeSH
- polymerázová řetězová reakce MeSH
- RNA ribozomální MeSH
- Sarcocystis klasifikace genetika izolace a purifikace MeSH
- sarkocystóza komplikace parazitologie veterinární MeSH
- svaly parazitologie MeSH
- Trichinella klasifikace genetika izolace a purifikace MeSH
- trichinelóza komplikace parazitologie veterinární MeSH
- vydry parazitologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
We present a novel case of an intestinal cestode infection in a southern sea otter (Enhydra lutris nereis). The cestode species Diphyllobothrium tetrapterum (syn. Diplogonoporus tetrapterus) was confirmed genetically. Stable isotope analysis of whiskers collected from the sea otter did not confirm the consumption of fish as the route of exposure.
- MeSH
- Cestoda klasifikace genetika izolace a purifikace MeSH
- cestodózy parazitologie veterinární MeSH
- DNA helmintů chemie izolace a purifikace MeSH
- fatální výsledek MeSH
- mitochondrie enzymologie MeSH
- respirační komplex IV genetika MeSH
- RNA ribozomální genetika MeSH
- střeva parazitologie MeSH
- vydry zranění parazitologie MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- Geografické názvy
- Kalifornie MeSH
The recent identification of Pseudamphistomum truncatum, (Rudolphi, 1819) (Trematoda: Opisthorchiidae) and Metorchis bilis (Braun, 1790) Odening, 1962 (synonymous with Metorchis albidus (Braun, 1893) Loos, 1899 and Metorchis crassiusculus (Rudolphi, 1809) Looss, 1899 (Trematoda: Opisthorchiidae)) in otters from Britain caused concern because of associated biliary damage, coupled with speculation over their alien status. Here, we investigate the presence, intensity and phylogeny of these trematodes in mustelids (principally otters) across Europe (Czech Republic, Denmark, France, Germany, Norway, Poland and Sweden and Britain). The trematodes were identified to species using the internal transcribed spacer II (ITS2) locus. Both parasites were found across Europe but at unequal frequency. In the German state of Saxony, eight out of eleven (73%) otters examined were infected with P. truncatum whilst this parasite was not found in either mink from Scotland (n=40) or otters from Norway (n=21). Differences in the phylogenies between the two species suggest divergent demographic histories possibly reflecting contrasting host diet or competitive exclusion, with M. bilis exhibiting greater mitochondrial diversity than P. truncatum. Shared haplotypes within the ranges of both parasite species probably reflect relatively unrestricted movements (both natural and anthropogenic) of intermediate and definitive hosts across Europe.
- MeSH
- fylogeneze MeSH
- haplotypy MeSH
- infekce červy třídy Trematoda epidemiologie parazitologie veterinární MeSH
- mitochondrie genetika MeSH
- norek parazitologie MeSH
- tok genů MeSH
- Trematoda klasifikace genetika izolace a purifikace MeSH
- vydry parazitologie MeSH
- zavlečené druhy MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Evropa epidemiologie MeSH
- Francie epidemiologie MeSH
- Německo epidemiologie MeSH
- Polsko epidemiologie MeSH
- Spojené království MeSH
