Metataxonomic approach was used to describe the bacterial community from a creosote-contaminated aquifer and to access the potential for in situ bioremediation of the polycyclic aromatic hydrocarbons (PAHs) by biostimulation. In general, the wells with higher PAH contamination had lower richness and diversity than others, using the Shannon and Simpson indices. By the principal coordinate analysis (PCoA) it was possible to observe the clustering of the bacterial community of most wells in response of the presence of PAH contamination. The significance analysis using edgeR package of the R program showed variation in the abundance of some Operational Taxonomic Units (OTUs) of contaminated wells compared to uncontaminated ones. Taxons enriched in the contaminated wells were correlated positively (p < 0.05) with the hydrocarbons, according to redundancy analysis (RDA). All these enriched taxa have been characterized as PAH degrading agents, such as the genus Comamonas, Geobacter, Hydrocarboniphaga, Anaerolinea and Desulfomonile. Additionally, it was possible to predict, with the PICRUSt program, a greater proportion of pathways and genes related to the degradation of PAHs in the wells with higher contamination levels. We conclude that the contaminants promoted the enrichment of several groups of degrading bacteria in the area, which strengthens the feasibility of applying biostimulation as an aquifer remediation strategy.

• MeSH
• Bacteria klasifikace   genetika   MeSH
• biodegradace MeSH
• kreosot analýza   MeSH
• mikrobiologie vody * MeSH
• mikrobiologie životního prostředí MeSH
• podzemní voda analýza   chemie   mikrobiologie   MeSH
• shluková analýza MeSH
• taxonomické DNA čárové kódování * MeSH
• těkavé organické sloučeniny MeSH
• uhlovodíky chemie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH