From 98 Lactobacillus strains, isolated from Algerian homemade cheeses, 14 (B1-B14) were selected based on their anti-Escherichia coli and anti-Staphylococcus aureus activities. These strains were also tested towards Listeria monocytogenes 161 and Salmonella Typhimurium LT2 and further investigated for their resistance to simulated gastrointestinal digestion, cell surface properties, ability to adhere to HT-29 cells, cholesterol lowering, antioxidant activity, and technological traits. Five isolates (B9, B13, B18, B19, and B38) were active against L. monocytogenes and Salmonella. From them, three isolates, identified as Lactobacillus brevis (B9, B13, and B38) by MALDI-TOF spectrometry and 16S rDNA sequencing, exhibited high tolerance to pancreatic juice, bile salts and acidic juices, high percentages of hydrophobicity (87, 92, and 81%, respectively), auto-aggregation (61, 68, and 72%, respectively), and adherence to HT-29 cells (79, 84, and 74%, respectively), which testify on their potential of colonization of the human intestine. On the other way, the strains B9 and B13 manifested the most relevant antioxidant activity and cholesterol-lowering ability, respectively. L. brevis strains showed low acidifying and good proteolytic activities with noticeable heat tolerance. The results gathered in this study highlighted the richness of Algerian artisanal cheeses on new lactobacilli strains with an excellent probiotic potential and demonstrated that L. brevis, largely used as nonstarter in cheese manufacture, could be exploited also as a probiotic for human use.

• MeSH
• antibióza MeSH
• bakteriální adheze MeSH
• buňky HT-29 MeSH
• Escherichia coli fyziologie   MeSH
• Levilactobacillus brevis * izolace a purifikace   fyziologie   MeSH
• lidé MeSH
• Listeria monocytogenes * fyziologie   MeSH
• potravinářská mikrobiologie * MeSH
• probiotika * izolace a purifikace   metabolismus   MeSH
• RNA ribozomální 16S genetika   MeSH
• Staphylococcus aureus fyziologie   MeSH
• sýr * mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH

It is demonstrated that numerous bacteria are able to attach to surfaces of equipment used for food handling or processing. In this study, a strain of Enterococcus durans, originally isolated from a milking machine surface, was firstly studied for its biofilm formation potential on plastic and stainless steel supports. The strain was found to be a biofilm producer either at 25, 30 or 37 °C on polystyrene microtitre plates, with a best adherence level observed at 25 °C. En. durans showed a strong adhesion to stainless steel AISI-304. Antibacterial and anti-adherence activities of En. durans were tested against four foodborne pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and Listeria innocua CLIP 74915) which were shown as biofilm producers on both plastic and stainless steel. En. durans cells and cell-free culture supernatant showed a significant (P < 0.05) inhibition potential of the pathogens either on solid media or in broth co-cultures. Characterization of the antibacterial substances indicated their proteinaceous nature which assigned them most probably to bacteriocins group.

• MeSH
• bakteriální adheze účinky léků   MeSH
• bakteriální proteiny metabolismus   MeSH
• biofilmy účinky léků   MeSH
• biologické přípravky metabolismus   MeSH
• Enterococcus metabolismus   fyziologie   MeSH
• gramnegativní bakterie účinky léků   fyziologie   MeSH
• grampozitivní bakterie účinky léků   fyziologie   MeSH
• mikrobiologie životního prostředí * MeSH
• nerezavějící ocel MeSH
• plastické hmoty MeSH
• potravinářská mikrobiologie MeSH
• teplota MeSH
• Publikační typ
• časopisecké články MeSH