Clastogens Dotaz Zobrazit nápovědu

855 záznamů v Medvik

Článek

The effects of exposure to different clastogens on the pattern of chromosomal aberrations detected by FISH whole chromosome painting in occupationally exposed individuals

Mutation research. 2006 ; 594 (1-2) : 20-29.

ISSN 0027-5107
Medvik
Zdroj

The pattern of chromosomal aberrations (CA) was studied by fluorescence in situ hybridization (FISH) technique (whole chromosomes #1 and #4 painting) in workers occupationally exposed to any of the four following conditions: acrylonitrile (ACN), ethyl benzene (EB), carcinogenic polycyclic aromatic hydrocarbons (c-PAHs), and irradiation in nuclear power plants (NPP), respectively. Decrease in the relative frequency of translocations was observed in EB group, and an increase in reciprocal translocations in ACN and NPP-exposed groups. An increase in a relative number of insertions was registered under all four conditions (significant at ACN, EB, c-PAHs, quasisignificant at NPP-exposed groups). Significant differences in the percentage of lymphocytes with aberrations on chromosome #1 (58.8+/-32.7%, versus 73.8+/-33.6% in the controls, P < 0.05), and chromosome #4 (47.0+/-34.1%, versus 29.4+/-32.2%, P < 0.01) were found in workers exposed to ACN. Similarly, a decrease in the proportion of cells with aberration on chromosome #1 (61.0+/-24.0%, versus 73.8+/-33.6%, P < 0.05) and an increase on chromosome #4 (45.6+/-24.6%, versus 29.4+/-32.2%, P < 0.05) were observed in workers exposed to EB. Frequency of aberrant cells (%AB.C.) as well as genomic frequency of translocations (F(G)/100) increased with age (P < 0.001). Aging also increased the percentage of translocations and reciprocal translocations (P < 0.05), but decreased the relative number of acentric fragments (P < 0.01). Smoking led to significantly increased F(G)/100 (P < 0.05), but did not affect the pattern of chromosomal aberrations. Our results seem to indicate that different carcinogens may induce a different pattern of chromosomal aberrations.

MeSH
akrylonitril toxicita MeSH
benzenové deriváty toxicita MeSH
chromozomální aberace chemicky indukované MeSH
dospělí MeSH
financování organizované MeSH
ionizující záření MeSH
kouření škodlivé účinky MeSH
kultivované buňky MeSH
lidé MeSH
lymfocyty účinky záření ultrasonografie MeSH
malování chromozomů MeSH
mutageny toxicita MeSH
polycyklické aromatické uhlovodíky toxicita MeSH
pracovní expozice škodlivé účinky MeSH
regresní analýza MeSH
Check Tag
dospělí MeSH
lidé MeSH
mužské pohlaví MeSH
Publikační typ
srovnávací studie MeSH

Článek

Chromosome aberrations in peripheral blood lymphocytes in subjects occupationally exposed to ionizing radiation or chemical clastogens

Folia biologica (Praha). 2002 ; Roč. 48 (č. 3) : s. 102-107.

ISSN 0015-5500
Medvik
Zdroj

MeSH
chromozomální aberace účinky léků účinky záření MeSH
lidé MeSH
lymfocyty metabolismus MeSH
poškození DNA účinky léků účinky záření MeSH
Check Tag
lidé MeSH

Abstrakt

Clastogenic effects in groups occupationally exposed to mutagens : Abstract

Rössner, P
Autor Rössner, P

21st Annual Meeting of EEMS on Environmental Mutagens - Carcinogens. 1991 ; () : S. S-9-6.

Medvik
Zdroj

MeSH
cytogenetika metody MeSH
karcinogeny MeSH
lidé MeSH
mutageny MeSH
nemoci z povolání prevence a kontrola MeSH
vystavení vlivu životního prostředí MeSH
Check Tag
lidé MeSH

Článek

Testing chemical agents with the cytokinesis-block micronucleus cytome assay

action: non-mutagens, direct-acting basealtering mutagens, direct-acting cross-linking mutagens, clastogens ...

Folia biologica (Praha). 2012 ; 58 (5) : 215-220.

Folia biol. (Praha)
ISSN 0015-5500
Medvik
Zdroj

In order to evaluate the applicability of the cytokinesis-block micronucleus cytome assay in routine mutagenicity testing we investigated with this method different chemicals having different mechanisms of action: non-mutagens, direct-acting basealtering mutagens, direct-acting cross-linking mutagens, clastogens including a radiomimetic chemical, indirect-acting spindle poisons and indirect-acting enzyme inhibitors. We looked at the presence of micronuclei as biomarkers for either the loss of chromosome fragments (clastogen) or the loss of a whole chromosome (aneugen), nucleoplasmic bridges as biomarkers for complex rearrangements (e.g., dicentric chromosomes) and nuclear buds as biomarkers for gene amplification. The cytome assay proved to be a suitable tool to investigate genetic effects of environmental agents and to provide insight into their working mechanisms as all chemicals tested showed the expected response.