We investigated the effect of a Multiwave Locked System laser (with a simultaneous 808 nm continuous emission and 905 nm pulse emission) on the spinal cord after spinal cord injury (SCI) in rats. The functional recovery was measured by locomotor tests (BBB, Beam walking, MotoRater) and a sensitivity test (Plantar test). The locomotor tests showed a significant improvement of the locomotor functions of the rats after laser treatment from the first week following lesioning, compared to the controls. The laser treatment significantly diminished thermal hyperalgesia after SCI as measured by the Plantar test. The atrophy of the soleus muscle was reduced in the laser treated rats. The histopathological investigation showed a positive effect of the laser therapy on white and gray matter sparing. Our data suggests an upregulation of M2 macrophages in laser treated animals by the increasing number of double labeled CD68+/CD206+ cells in the cranial and central parts of the lesion, compared to the control animals. A shift in microglial/macrophage polarization was confirmed by gene expression analysis by significant mRNA downregulation of Cd86 (marker of inflammatory M1), and non-significant upregulation of Arg1 (marker of M2). These results demonstrated that the combination of 808 nm and 905 nm wavelength light is a promising non-invasive therapy for improving functional recovery and tissue sparing after SCI.
- MeSH
- B7-2 Antigen genetics metabolism MeSH
- Antigens, Differentiation, Myelomonocytic genetics metabolism MeSH
- Antigens, CD genetics metabolism MeSH
- Rats MeSH
- Low-Level Light Therapy methods MeSH
- Lectins, C-Type genetics metabolism MeSH
- Mannose-Binding Lectins genetics metabolism MeSH
- Locomotion MeSH
- Spinal Cord metabolism pathology MeSH
- Spinal Cord Injuries therapy MeSH
- Rats, Wistar MeSH
- Receptors, Cell Surface genetics metabolism MeSH
- Spinal Cord Regeneration MeSH
- Animals MeSH
- Check Tag
- Rats MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Keywords
- překonané Arndt-Schulzovo pravidlo, Hight Laser Intensity Therapy, Multiwave Locked System,
- MeSH
- General Surgery MeSH
- Low-Level Light Therapy * history classification methods standards instrumentation utilization MeSH
- Laser Therapy methods utilization MeSH
- Humans MeSH
- Check Tag
- Humans MeSH
- Publication type
- Newspaper Article MeSH
