Propagation strategy
Dotaz
Zobrazit nápovědu
Autori opisujú novú víziu SZO: Svet bez TBC, v rámci Stop TBC Stiatégie, prijatej na roky 2006-2015. Zároveň opisujú aktivity členov Slovenskej pneumologickej a ftizeologickej spoločnosti na poli propagácie stratégie SZO: Zdravie pre všetkých v 21. storočí. Hodnotia 21 článkov, na ktorých sa podieľali členovia Slovenskej pneumologickej a ftizeologickej spoločnosti a oceňujú postoje k prioritným cielom pre 21. storočie vo vybraných komunitách zdravotníckych pracovníkov. Tento prieskum bude velmi dôležitý pri vyhodnocovaní stratégie SZO a vypracovaní edukačnej stratégie v budúcich rokoch.
Authors presents the new strategy of the WHO The Stop TB Strategy for tiie years 2006-2015. They describe activity of the Slovak pnemological and phthiseological association in field of propagation the WHO Strategy Health for All in the 21st century. They evaluate 21 articles in which the members are participated and highly appreciate investigation of attitudes targets for the 21st century. This investigation will be important in evaluation of WHO Strategy and elaboration of the educational strategy for the next years.
- MeSH
- lidé MeSH
- státní zdravotnické plány MeSH
- Světová zdravotnická organizace organizace a řízení MeSH
- tuberkulóza prevence a kontrola terapie MeSH
- Check Tag
- lidé MeSH
- Geografické názvy
- Slovenská republika MeSH
Aim: Anxiety is prevalent among women with Hyperemesis Gravidarum (HG), which is associated with coping strategies and bio-psychosocial risk factors. This study aims to predict socioeconomic status (SES) and obstetric factors towards anxiety and stress-coping strategy, and to explore the significant differences in levels of anxiety on each coping strategy. Design: It was a cross-sectional survey in the Malaysian context. Methods: Forty women medically diagnosed with HG were recruited and completed Generalized Anxiety Disorder-7 (GAD-7) to assess generalized anxiety disorder and coping scale inventory using the Brief COPE questionnaire. Results: The employment status of pregnant women diagnosed with HG significantly predicted their anxiety. Household income and education level predicted emotion-focused coping strategies. None of the obstetric factors predicted the coping strategy. A significant difference was found across anxiety levels in problem-focused and dysfunctional coping strategies. The post-hoc test reveals that dysfunctional coping has the highest mean score in the severe anxiety category. Conclusion: It is essential to promote awareness about effective coping strategies for pregnant women who are diagnosed with HG. Greater efforts are needed to increase the duty of care among healthcare providers to propagate holistic and resourceful coping management with the HG that could help potential patients in the future.
- MeSH
- adaptace psychologická MeSH
- hyperemesis gravidarum * psychologie MeSH
- klinická studie jako téma MeSH
- lidé MeSH
- těhotenství MeSH
- úzkost MeSH
- Check Tag
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Geografické názvy
- Malajsie MeSH
The partial-filling affinity capillary electrophoresis (pf-ACE) works with a ligand present in a background electrolyte that forms a weak complex with an analyte. In contrast to a more popular mobility-shift affinity capillary electrophoresis, only a short plug of the ligand is introduced into a capillary in the pf-ACE. Both methods can serve for determining apparent stability constants of the formed complexes but this task is hindered in the pf-ACE by the fact that the analyte spends only a part of its migration time in a contact with the ligand. In 1998, Amini and Westerlund published a linearization strategy that allows for extracting an effective mobility of an analyte in the presence of a neutral ligand out of the pf-ACE data. The main purpose of this paper is to show that the original formula is only approximate. We derive a new formula and demonstrate its applicability by means of computer simulations. We further inspect several strategies of data processing in the pf-ACE regarding a risk of an error propagation. This establishes a good practice of determining apparent stability constants of analyte-ligand complexes by means of the pf-ACE.
The aim of this investigation was to determine the persistence of biofilm-associated antibiotic resistance developed by methicillin-sensitive Staphylococcus aureus (MSSA), of different capsular types, during biofilm formation. Because of superiority of the tissue culture plate (TCP) over the Congo Red Agar (CRA) method for measuring biofilm formation, it was used to determine the persistence of the antibiotic resistance developed by the isolates in biofilms. The antibiotic resistance was found to persist for 3-4 wk post-propagation as planktonic subcultures. Interestingly, some strains even developed resistance to vancomycin and/or teicoplanin. However, no association of either biofilm formation or persistent antibiotic resistance with the major capsular phenotype was observed. These observations highlight the potential significance of (a) determining the antibiograms of S. aureus subcultured from biofilms developed in vitro using the TCP method as well as from planktonic cultures for formulation of an optimal therapeutic strategy, and (b) continuing to identify predominant non-capsular antigens contributing to biofilm formation, regardless of the capsular phenotype for the development of an effective potentially broad-spectrum vaccine for prevention of bovine mastitis caused by S. aureus.
- MeSH
- antibakteriální látky farmakologie MeSH
- bakteriální léková rezistence * MeSH
- bakteriální pouzdra genetika MeSH
- bakteriální proteiny MeSH
- biofilmy účinky léků růst a vývoj MeSH
- genotyp MeSH
- mastitida skotu mikrobiologie MeSH
- mikrobiální testy citlivosti MeSH
- polymerázová řetězová reakce MeSH
- skot MeSH
- Staphylococcus aureus účinky léků izolace a purifikace fyziologie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Austrálie MeSH
In the carnivorous plant Venus flytrap (Dionaea muscipula), the sequence of events after prey capture resembles the well-known plant defence signalling pathway in response to pathogen or herbivore attack. Here, we used wounding to mimic prey capture to show the similarities and differences between botanical carnivory and plant defence mechanisms. We monitored movement, electrical signalling, jasmonate accumulation and digestive enzyme secretion in local and distal (systemic) traps in response to prey capture, the mechanical stimulation of trigger hairs and wounding. The Venus flytrap cannot discriminate between wounding and mechanical trigger hair stimulation. Both induced the same action potentials, rapid trap closure, hermetic trap sealing, the accumulation of jasmonic acid (JA) and its isoleucine conjugate (JA-Ile), and the secretion of proteases (aspartic and cysteine proteases), phosphatases and type I chitinase. The jasmonate accumulation and enzyme secretion were confined to the local traps, to which the stimulus was applied, which correlates with the propagation of electrical signals and the absence of a systemic response in the Venus flytrap. In contrast to plant defence mechanisms, the absence of a systemic response in carnivorous plant may represent a resource-saving strategy. During prey capture, it could be quite expensive to produce digestive enzymes in the traps on the plant without prey.
Duckweeds are aquatic monocotyledonous plants of potential economic interest with fast vegetative propagation, comprising 37 species with variable genome sizes (0.158-1.88 Gbp). The genomic sequence of Spirodela polyrhiza, the smallest and the most ancient duckweed genome, needs to be aligned to its chromosomes as a reference and prerequisite to study the genome and karyotype evolution of other duckweed species. We selected physically mapped bacterial artificial chromosomes (BACs) containing Spirodela DNA inserts with little or no repetitive elements as probes for multicolor fluorescence in situ hybridization (mcFISH), using an optimized BAC pooling strategy, to validate its physical map and correlate it with its chromosome complement. By consecutive mcFISH analyses, we assigned the originally assembled 32 pseudomolecules (supercontigs) of the genomic sequences to the 20 chromosomes of S. polyrhiza. A Spirodela cytogenetic map containing 96 BAC markers with an average distance of 0.89 Mbp was constructed. Using a cocktail of 41 BACs in three colors, all chromosome pairs could be individualized simultaneously. Seven ancestral blocks emerged from duplicated chromosome segments of 19 Spirodela chromosomes. The chromosomally integrated genome of S. polyrhiza and the established prerequisites for comparative chromosome painting enable future studies on the chromosome homoeology and karyotype evolution of duckweed species.
- MeSH
- Araceae genetika MeSH
- chromozomy rostlin genetika MeSH
- fyzikální mapování chromozomů MeSH
- genom rostlinný genetika MeSH
- genomika MeSH
- hybridizace in situ fluorescenční MeSH
- karyotyp MeSH
- karyotypizace MeSH
- molekulární evoluce MeSH
- umělé bakteriální chromozomy MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
... physical therapy geriatric rehabilitation 20 -- Tools and methods to evaluate errors and their propagation ... ... phases 52 -- Vestibular adaptation: effects of acceleration and velocity pulses on perception 53 -- Strategy ...
78 stran : ilustrace ; 30 cm
... -- VI TECHNIQUES AND OTHER TOPICS IN DC BIOLOGY -- CHAPTER 27 A Guide to the Isolation and 515 Propagation ... ... Young and Nikolaus Romani -- CHAPTER 28 Propagation and Culture of Dendritic 535 Cells \' Shannon J. ...
xxvii, 733 stran : ilustrace; 25 cm
- MeSH
- dendritické buňky MeSH
- Konspekt
- Biochemie. Molekulární biologie. Biofyzika
- NLK Obory
- biochemie
- biologie
- NLK Publikační typ
- kolektivní monografie
BACKGROUND: Since the Substrate and Trigger Ablation for Reduction of Atrial Fibrillation Trial Part II (STAR-AF II), there has been a trend toward pulmonary vein isolation (PVI)-only ablation strategies for persistent atrial fibrillation (PeAF). Electrographic flow (EGF) mapping can identify active sources of atrial fibrillation (AF) and estimate the electrographic flow consistency (EGFC) of wavefront propagation through substrate, revealing functional AF mechanisms. OBJECTIVE: We sought to examine the success of a PVI-only ablation strategy for a redo PeAF/longstanding PeAF population. METHODS: Electrographic Flow-Guided Ablation in Redo Patients With Persistent Atrial Fibrillation (FLOW-AF [NCT04473963]) prospectively enrolled patients with nonparoxysmal AF undergoing redo ablation at 4 centers. One-minute EGF recordings using 64-pole basket catheters were obtained both pre-PVI and post-PVI following a 20-minute wait and confirmation of electrical isolation of veins. Patients with EGF-identified sources were randomized 1:1 to EGF-guided source ablation vs PVI-only. Patients with no sources were not randomized and mostly received PVI only. RESULTS: Study of 85 patients enrolled 24 with EGF-identified sources randomized to PVI only and 23 with no sources receiving PVI only. Of these 47 patients, those with sources (Group 2) had different clinical characteristics including older age and higher CHA2DS2-VASc scores compared with those with no sources (Group 1). After PVI only, Group 1 had 70% (16 of 23) freedom from recurrent AF (FFAF) within 1 year vs Group 2 with 35% (8 of 23), P = .018. In addition, patients with high electrographic flow consistency (EGFC) indicative of healthy or normal substrate had 67% (10 of 15) FFAF vs 45% (14 of 31) in those with low EGFC suggestive of abnormal substrate, P = .011. CONCLUSION: Success rates in no-sources patients receiving PVI only are better than in those with sources randomized to PVI only. For the clinically heterogenous population of patients with PeAF, the presence of EGF-identified sources matters clinically, and PVI only will not be enough for all patients.
- MeSH
- fibrilace síní * chirurgie patofyziologie diagnóza MeSH
- katetrizační ablace * metody MeSH
- lidé středního věku MeSH
- lidé MeSH
- následné studie MeSH
- převodní systém srdeční * patofyziologie chirurgie MeSH
- prospektivní studie MeSH
- senioři MeSH
- venae pulmonales * chirurgie patofyziologie MeSH
- výsledek terapie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- multicentrická studie MeSH
- randomizované kontrolované studie MeSH
