Semecky, V* Dotaz Zobrazit nápovědu
The collapse of vimentin caused by some xenobiotics correlates with the loss of structural integrity of the seminiferous epithelium. In this study, we investigated the effect of busulphan (an anticancer drug with toxic effects on dividing germ cells) on vimentin filament distribution in rat seminiferous epithelium and compared it with changes found in testes of unilaterally cryptorchid rats. In the seminiferous epithelium, the vimentin labelling was observed only in the Sertoli cells, showing a stage-specific arrangement of the filaments. Both busulphan treatment and cryptorchism caused altered distribution of vimentin filaments in the Sertoli cells. In both models, the apical vimentin filaments collapsed towards the nuclei and were disorganized in the basal region of the Sertoli cells while the germ cells were diminished in the epithelium. After the busulphan effect subsided (4 weeks after administration), spermatogenesis began to restore and vimentin filaments began to organize in basal and perinuclear regions of Sertoli cells among the spermatogonia and spermatocytes. Vimentin labelling of the sloughed material in the lumen of cryptorchid testes (but not in busulphan treated animals) was observed. We conclude that the Sertoli cell vimentin filaments play an important role in the maintenance of spermatogenesis, their damage is associated with the seminiferous epithelium disintegration and their restoration with a recovery of spermatogenesis after the unfavourable conditions subside.
- MeSH
- busulfan farmakologie MeSH
- časové faktory MeSH
- financování organizované MeSH
- imunohistochemie MeSH
- intermediární filamenta chemie metabolismus ultrastruktura MeSH
- krysa rodu rattus MeSH
- potkani Wistar MeSH
- semenný epitel metabolismus ultrastruktura MeSH
- spermatogeneze fyziologie genetika účinky léků MeSH
- testis metabolismus ultrastruktura MeSH
- velikost orgánu MeSH
- vimentin biosyntéza metabolismus účinky léků MeSH
- vztah mezi dávkou a účinkem léčiva MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- MeSH
- aminokapronáty MeSH
- azepiny farmakologie MeSH
- chemická stimulace MeSH
- indikátory a reagencie MeSH
- kožní absorpce účinky léků MeSH
- králíci MeSH
- kyselina 6-aminokapronová farmakologie chemická syntéza MeSH
- LD50 MeSH
- lidé MeSH
- myši MeSH
- techniky in vitro MeSH
- theofylin farmakokinetika MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- srovnávací studie MeSH
- MeSH
- intestinální absorpce MeSH
- krysa rodu rattus MeSH
- malabsorpční syndromy MeSH
- tenké střevo MeSH
- xylosa diagnostické užití MeSH
- Check Tag
- krysa rodu rattus MeSH
- Publikační typ
- srovnávací studie MeSH
