antimycin A Dotaz Zobrazit nápovědu
In this research, a microbial endophytic strain obtained from the rhizosphere of the conifer Taxus baccata and designated as Streptomyces sp. AC35 (FJ001754.1 Streptomyces, GenBank) was investigated. High 16S rDNA gene sequence similarity suggests that this strain is closely related to S. odorifer. The major fatty acid profile of intracellular lipids was also carried out to further identify this strain. Atomic force microscopy and scanning acoustic microscopy were used to image our strain. Its major excreted substances were extracted, evaluated for antimicrobial activity, purified, and identified by ultraviolet-visible spectroscopy (UV-vis), liquid chromatography-mass spectrometry (LC-MS/MS) and nuclear magnetic resonance as the bioactive isoflavone aglycones-daidzein, glycitein and genistein. Batch cultivation, performed under different pH conditions, revealed enhanced production of antimycin components when the pH was stable at 7.0. Antimycins were detected by HPLC and identified by UV-vis and LC-MS/MS combined with the multiple reaction monitoring. Our results demonstrate that Streptomyces sp. AC35 might be used as a potential source of effective, pharmaceutically active compounds.
- MeSH
- antimycin A farmakologie MeSH
- glycerolfosfátdehydrogenasa antagonisté a inhibitory metabolismus MeSH
- glycerolfosfáty metabolismus MeSH
- hnědá tuková tkáň enzymologie MeSH
- křečci praví MeSH
- mitochondrie enzymologie MeSH
- ubichinon metabolismus MeSH
- zvířata MeSH
- Check Tag
- křečci praví MeSH
- zvířata MeSH
Membrane fragments of two mutant strains of Paracoccus denitrificans genetically modified in the bc(1) complex have been studied for comparison of enzymic activities of succinate-cytochrome-c reductase and its components, viz. succinate dehydrogenase (Complex II) and ubiquinol-cytochrome-c reductase (Complex III) and their response to changes in concentration of succinate, cytochrome c, ionic strength, pH, temperature and sensitivity to antimycin A. The mutants synthesized and assembled the b and c hemes in the ratio characteristic for the wild type strain. The mutant strain M 71 expressing the truncated copy of cytochrome c(1) (devoid of a stretch of 150 mainly acidic amino acids) was less sensitive to increasing concentration of cytochrome c and changes in ionic strength of the medium, but maintained the original affinity to succinate and sensitivity to antimycin A. The mutant strain M 36 with an overexpressed bc(1) content showed the highest response to changes in ionic strength and physical parameters, exhibited the lowest turnover number values with succinate-cytochrome-c reductase, but positively affected the succinate dehydrogenase. In view of the interaction of the redox components in native membranes the functional analyses of separated Complexes II and III should be regarded with caution.
- MeSH
- antibakteriální látky farmakologie MeSH
- antimycin A farmakologie MeSH
- buněčná membrána enzymologie metabolismus MeSH
- elektrony MeSH
- fyziologický stres MeSH
- koncentrace vodíkových iontů MeSH
- mutace MeSH
- operon MeSH
- osmotický tlak MeSH
- oxidace-redukce MeSH
- Paracoccus denitrificans metabolismus MeSH
- respirační komplex III MeSH
- sukcinátcytochrom c oxidoreduktasa MeSH
- teplota MeSH
