Here, we report synthesis and biological evaluation of fluorescent nandrolone-3-carboxymethyloxime derivatives conjugated with green-emitting bodipy dye via PEG linkers. All the newly-synthesized compounds were evaluated for their effect on cell proliferation in vitro in MCF-7, LNCaP, PC-3 and HEK 293T model cell lines using WST-1 assay. By means of live-cell fluorescence microscopy, the intracellular localization of nandrolone-bodipy conjugates was revealed in endoplasmic reticulum. Moreover, we performed competitive localization study with nonfluorescent nandrolone, metandrolone, boldenone, trenbolone, and testosterone.

• MeSH
• fluorescenční barviva chemická syntéza   chemie   MeSH
• fluorescenční mikroskopie MeSH
• HEK293 buňky MeSH
• HeLa buňky MeSH
• lidé MeSH
• MFC-7 buňky MeSH
• molekulární konformace MeSH
• nádorové buněčné linie MeSH
• nandrolon chemie   farmakologie   MeSH
• proliferace buněk účinky léků   MeSH
• sloučeniny boru chemie   farmakologie   MeSH
• vztah mezi dávkou a účinkem léčiva MeSH
• vztahy mezi strukturou a aktivitou MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH