- MeSH
- Cells cytology immunology pathology MeSH
- DNA, Neoplasm genetics MeSH
- Research Support as Topic MeSH
- Hyperthermia, Induced methods utilization MeSH
- Nuclear Matrix genetics MeSH
- Humans MeSH
- Neoplastic Processes MeSH
- Drug Delivery Systems utilization MeSH
- Check Tag
- Humans MeSH
- Publication type
- Comparative Study MeSH
Journal of cellular biochemistry, ISSN 0730-2312 suppl. 35, 2000
157 s. : il., tab. ; 32 cm
- Conspectus
- Biochemie. Molekulární biologie. Biofyzika
- NML Fields
- biochemie
- biologie
- onkologie
In order to gain further insights into the fundamental structure of the nucleolus, nucleolar remnants of Xenopus and chickens were examined for the presence of fibrillarin and nucleolus organizer region (NOR) silver staining. Nucleolar remnants of Xenopus nucleated red blood cells were found to contain easily detectable amounts of fibrillarin and NOR silver staining. Upon examination of various tissues, fibrillarin and NOR silver staining were detected in nucleoli of Xenopus liver hepatocytes and within nucleoli of oocytes and follicle cells from ovaries of mature female toads. By comparison, nucleolar remnants of adult chicken nucleated red blood cells contained only trace amounts of fibrillarin and NOR silver staining, whereas red blood cell nucleolar remnants of immature chicks had easily detectable amounts of fibrillarin and NOR silver staining. Nucleoli from hepatocytes of both adult and immature chickens demonstrated comparable levels of fibrillarin and NOR silver staining. Since fibrillarin was found in nucleolar remnant structures, we tested for (and detected) its presence in residual nucleoli of in situ nuclear matrix derived from HeLa cells. These findings are discussed in terms of the basic structural and functional organization of the nucleolus.
- MeSH
- Cell Nucleolus * ultrastructure MeSH
- Chromosomal Proteins, Non-Histone * analysis MeSH
- Microscopy, Electron methods MeSH
- Erythrocytes ultrastructure MeSH
- Fluorescent Antibody Technique MeSH
- HeLa Cells MeSH
- Nuclear Matrix ultrastructure MeSH
- Liver ultrastructure MeSH
- Cells, Cultured MeSH
- Chickens MeSH
- Humans MeSH
- Oocytes ultrastructure MeSH
- Nucleolus Organizer Region * ultrastructure MeSH
- Ribonucleoproteins analysis MeSH
- Xenopus laevis MeSH
- Animals MeSH
- Check Tag
- Humans MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Research Support, U.S. Gov't, P.H.S. MeSH
- Comparative Study MeSH