- MeSH
- Ceruloplasmin blood MeSH
- Rats MeSH
- Silver metabolism MeSH
- Check Tag
- Rats MeSH
- MeSH
- Ceruloplasmin MeSH
- Animal Experimentation MeSH
- Hydrogen-Ion Concentration MeSH
- Blood MeSH
- Humans MeSH
- Check Tag
- Humans MeSH
- MeSH
- Ceruloplasmin MeSH
- Estrogens MeSH
- Hypothalamus enzymology MeSH
- Rats MeSH
- Oxidoreductases MeSH
- Silver MeSH
- Check Tag
- Rats MeSH
Elicitácia rastlinných in vitro kultúr predstavuje biotechnologický nástroj na zvýšenie produkcie sekundárnych metabolitov. V tejto práci bol sledovaný vplyv elicitácie dusičnanom strieborným a chloridom kademnatým na produkciu sanguinarínu suspenznou kultúrou slncovky kalifornskej. Elicitory boli ku kultúram pridané na 14. deň subkultivácie a ich vplyv na tvorbu sanguinarínu bol vyhodnotený po 48 h pôsobení. AgNO3 v koncentrácii 0,075 mmol.l-1 a CdCl2 v koncentrácii 4 mmol.l-1 indukovali približne 5,2-, resp. 5,6-násobné zvýšenie tvorby sanguinarínu. Toto zvýšenie pravdepodobne predstavuje maximálny nárast, pretože zvýšenie koncentrácie elicitorov už k ďalšiemu nárastu produkcie sanguinarínu neviedlo. Použitie obidvoch elicitorov viedlo k zvýšeniu špecifickej aktivity polyfenoloxidázy. Polyfenoloxidáza je pravdepodobne zapojená do biosyntézy sanguinarínu na úrovni tvorby dopamínu, ktorý je prekurzorom S-norkoklaurínu, prvého intermediátu s benzylizochinolínovou štruktúrou.
Elicitation of plant in vitro cultures represents a biotechnological tool to improve the production of secondary metabolites. In this study, the effect of AgNO3 and CdCl2 on the sanguinarine production by the suspension culture of Eschscholtzia californica CHAM. was investigated. Elicitors were added to the cultures at the 14th day of subcultivation and their effect on the sanguinarine production was evaluated after a 48 h exposure. AgNO3 at the concentration of 0.075 mmol.l-1 and CdCl2 at the concentration of 4 mmol.l-1 induced a ca. 5.2- and 5.6-multiple increase in sanguinarine synthesis, respectively. This amount represents probably the maximal production, because a further increase in the elicitors concentrations did not increase sanguinarine production. Both abiotic elicitors induced a polyphenol oxidase specific activity increase. Polyphenol oxidase is probably involved in the biosynthesis of sanguinarine at the level of dopamine formation. Dopamine is a precursor of (S)-norcoclaurine, the first intermediate with the benzylisoquinoline structure.
- MeSH
- Anti-Bacterial Agents MeSH
- Benzophenanthridines * pharmacology chemistry isolation & purification MeSH
- Biotechnology methods instrumentation trends MeSH
- Chemistry Techniques, Analytical methods utilization MeSH
- Chemistry, Pharmaceutical * MeSH
- Eschscholzia * chemistry MeSH
- Technology, Pharmaceutical MeSH
- Phenols analysis MeSH
- Analytic Sample Preparation Methods MeSH
- Plants MeSH
- Suspensions MeSH
- In Vitro Techniques MeSH
- Tissue Culture Techniques * MeSH
BACKGROUND: Polyphenols and phenolic acid are able to slow down or prevent oxidation processes and are therefore thought to have important effects in malting and brewing. Laccase catalyses the oxidation of a wide variety of substrates, including polyphenols. The aim of this paper was to determine the changes in polyphenol compounds and the relative expression of the HvLac1 gene during malting. RESULTS: The dominant phenolic acid was ferulic acid. The amount of ferulic acid increased, whereas the amount of vanillic acid decreased during malting. The highest levels of expression of the HvLac1 gene were observed during the third air rest period in varieties with the 'Haruna Nijo' (HN) allele, as recommended for the production of beer with the protected geographical indication (PGI) 'Česke pivo' (Czech beer), whereas the highest expression was observed in the first day of germination in varieties with the 'Morex' (M) allele. However, the profiles of HvLac1 gene expression in varieties with alternative alleles during malting were similar, and the level of polyphenol compounds throughout malting was different. CONCLUSION: The polyphenol contents in barley increased several-fold during malting, and the degree of increase differed with variety. The expression of HvLac1 transcript was similar in every barley variety.
- MeSH
- Hordeum enzymology MeSH
- Coumaric Acids chemistry metabolism MeSH
- Laccase genetics metabolism MeSH
- Polyphenols chemistry MeSH
- Gene Expression Regulation, Enzymologic physiology MeSH
- Gene Expression Regulation, Plant physiology MeSH
- Seedlings enzymology MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
AIM OF THE STUDY: To investigate in vitro xanthine oxidase inhibitory properties of plants traditionally used in Czech Republic and Central-East Europe region for gout, arthritis or rheumatism treatment. MATERIALS AND METHODS: Methylene chloride-methanolic and two ethanolic extracts of 27 plant species were screened for in vitro xanthine oxidase inhibitory activity using a spectrophotometric method. RESULTS: Around 50% of the species exhibited some degree of xanthine oxidase inhibitory properties at 200 μg/mL, showing a moderate correlation (r=0.59) with total phenol content. The most active were methylene chloride-methanolic extracts of Populus nigra and Betula pendula, with IC(50) of 8.3 and 25.9 μg/mL, respectively, followed by 80% ethanolic extract of Caryophyllus aromaticus and Hypericum perforatum, both under 50 μg/mL. CONCLUSIONS: Populus nigra and Betula pendula were identified as species with the highest xanthine oxidase inhibitory potential in our study. This correlates with the ethnobotanical data on their use in Central European folklore and provides the basis for further investigation on these plants.
- MeSH
- Betula metabolism MeSH
- Ethnopharmacology MeSH
- Phenols analysis pharmacology MeSH
- Flavonoids analysis pharmacology MeSH
- Enzyme Inhibitors pharmacology MeSH
- Plants, Medicinal MeSH
- Polyphenols MeSH
- Populus MeSH
- Plant Extracts pharmacology MeSH
- Xanthine Oxidase antagonists & inhibitors MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Geographicals
- Czech Republic MeSH
