rutin
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- MeSH
- azbest škodlivé účinky MeSH
- experimenty na zvířatech MeSH
- instilace léků MeSH
- křemen škodlivé účinky MeSH
- měď terapeutické užití MeSH
- modely nemocí na zvířatech MeSH
- plicní fibróza MeSH
- potkani Wistar MeSH
- rutin terapeutické užití MeSH
- suspenze MeSH
- trachea MeSH
- zvířata MeSH
- Check Tag
- ženské pohlaví MeSH
- zvířata MeSH
Rutin is a natural antioxidant useful for human health. The aim of this study was to compare some analytical methods for rutin determination in a plant material ? tartary buckwheat (Fagopyrum tataricum (L.) Gaertn.) ? of different origin. The methods used were HPLC/UV and two spectrophotometries. Both spectrophotometric methods gave higher values than HPLC/UV. The results mutually correlated well, except those obtained by the spectrophotometric method using AlCl3. The method proved unsuitable for the purpose. The other spectrophotometric method overestimated the rutin content in leaves and stems.
The mechanism of electrochemical oxidation of rutin on a glassy carbon electrode was studied at different pH by using several electrochemical techniques (cyclic, linear sweep, differential pulse and square-wave voltammetry) in order to give deeper insight into the mechanism of electrochemical oxidation of rutin and adsorption of its oxidation products on a glassy carbon electrode. It was determined that the rutin oxidation process on a glassy carbon electrode is reversible, pH dependent and includes the transfer of 2 e– and 2 H+. The products of electrochemical oxidation strongly adsorb on the electrode surface. Maximum surface coverage, ?max, decreased with increasing scan rate from 3.4 × 10–9 mol cm–2 at scan rate 20 mV s–1 to 1.5 × 10–9 mol cm–2 at scan rate 100 mV s–1 and adsorption equilibrium constant was log K = 4.57 ± 0.05. Antioxidant properties of rutin were investigated by a Trolox equivalent antioxidant capacity (TEAC) assay. It was found that the TEAC values of rutin depend on concentration and the EC50 value of rutin amounted 0.23.
The performances of ionic liquid (1-hexyl-3-methylimidazolium-bis(trifluoromethylsulfonyl)imide, IL/CPE) and iron phthalocyanine (IP/CPE) modified carbon paste electrodes in electroanalytical determinations of rutin were evaluated and compared to the performance of unmodified carbon paste electrode (CPE). Cyclic voltammetry (CV), differential pulse voltammetry (DPV), differential pulse adsorptive stripping voltammetry (DPAdSV), and amperometry were used for rutin analysis. The best current responses of rutin were obtained at pH 4.0 for all tested techniques. IL/CPE electrode was found to perform best with DPAdSV technique, where a detection limit (LOD) as low as 5 nmol L(-1) of rutin was found. On the other hand, IP/CPE showed itself to be an optimum choice for DPV technique, where LOD of 80 nmol L(-1) was obtained. Analytical applicability of newly prepared electrodes was demonstrated on determination of rutin in the model samples and the extracts of buckwheat seeds. To find an optimum method for buckwheat seeds extraction, a boiling water extraction (BWE), Soxhlet extraction (SE), pressurized solvent extraction (PSE), and supercritical fluid extraction (SFE) were tested.
- MeSH
- elektrochemie metody MeSH
- elektrody MeSH
- Fagopyrum chemie MeSH
- molekulární struktura MeSH
- rutin chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Cílem přehledného článku bylo dosáhnout hlubšího pochopení ve vědeckých časopisech publikovaných účinků flavonolů, zejména rutinu, kvercitrinu a jejich aglykonu kvercetinu, s důrazem na jejich potenciální příznivé působení u nespecifických střevních zánětů. Výzkum v oblasti flavonoidů v několika posledních létech významně vzrostl a objevila se řada nových skutečností týkajících se jejich absorpce, metabolismu a možných mechanismů účinků. Článek uvádí nedávno publikované výsledky získané v pokusech in vitro a experimentech in vivo na zvířecích modelech. Další výzkum zaměřený na klinické účinky rutinu může být důležitý pro vývoj nové léčivé látky k terapii nespecifických střevních zánětů v humánní medicíně.
The aim of this review article was to obtain a deeper understanding of the reported effects of flavonols, in particular rutin, quercitrin and their aglycone quercetin, with respect to their potential beneficial action on inflammatory bowel disease. Research in the field of flavonoids has increased significantly in recent few years and many new investigations have been performed concerning their absorption, metabolism, and probable mechanisms of action. Recently published results obtained in in vitro approaches and in vivo experiments on animal models are reported. Further investigation aimed at the clinical effects of rutin may be important for the development of a new therapeutic agent for the treatment of inflammatory bowel disease in humans.
- MeSH
- experimenty na zvířatech MeSH
- financování organizované MeSH
- flavonoidy aplikace a dávkování farmakologie MeSH
- isoprenalin diagnostické užití toxicita MeSH
- kardiotoxiny diagnostické užití toxicita MeSH
- kardiovaskulární nemoci etiologie farmakoterapie MeSH
- katecholaminy diagnostické užití toxicita MeSH
- potkani Wistar MeSH
- reaktivní formy kyslíku farmakologie MeSH
- rutin diagnostické užití MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- abstrakty MeSH
Jasmonates are plant hormones that induce the accumulation of many secondary metabolites, such as rutin in buckwheat, via regulation of jasmonate-responsive transcription factors. Here, we report on the identification of a clade of jasmonate-responsive subgroup 4 MYB transcription factors, FtMYB13, FtMYB14, FtMYB15, and FtMYB16, which directly repress rutin biosynthesis in Fagopyrum tataricum. Immunoblot analysis showed that FtMYB13, FtMYB14, and FtMYB15 could be degraded via the 26S proteasome in the COI1-dependent jasmonate signaling pathway, and that this degradation is due to the SID motif in their C-terminus. Yeast two-hybrid and bimolecular fluorescence complementation assays revealed that FtMYB13, FtMYB14, and FtMYB15 interact with the importin protein Sensitive to ABA and Drought 2 (FtSAD2) in stem and inflorescence. Furthermore, the key repressor of jasmonate signaling FtJAZ1 specifically interacts with FtMYB13. Point mutation analysis showed that the conserved Asp residue of the SID domain contributes to mediating protein-protein interaction. Protoplast transient activation assays demonstrated that FtMYB13, FtMYB14, and FtMYB15 directly repress phenylalanine ammonia lyase (FtPAL) gene expression, and FtSAD2 and FtJAZ1 significantly promote the repressing activity of FtMYBs. These findings may ultimately be promising for further engineering of plant secondary metabolism.
- MeSH
- cyklopentany metabolismus MeSH
- Fagopyrum chemie genetika metabolismus MeSH
- fenylalaninamoniaklyasa genetika metabolismus MeSH
- multigenová rodina MeSH
- oxylipiny metabolismus MeSH
- proteinové domény MeSH
- regulace genové exprese u rostlin MeSH
- regulátory růstu rostlin metabolismus MeSH
- rostlinné proteiny chemie genetika metabolismus MeSH
- rutin biosyntéza MeSH
- transkripční faktory chemie genetika metabolismus MeSH
- vazba proteinů MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Buckwheat leaves and young parts of the plant are consumed in some countries as a vegetable. Green flour, obtained by milling of the dried plants, is used as a natural food colorant. The distribution of vitamin E, squalene, epicatechin, and rutin (as the most important antioxidants) within buckwheat plants, as well as changes of their content within leaves during the growing season, were determined by GC-MS and HPLC analyses. alpha-Tocopherol was found as the main component of vitamin E in all parts of the plant; epicatechin and squalene were also detected. For the use of buckwheat as an antioxidant source in the human diet, the most suitable part of the plants seems to be the leaves and the flowers at the stage of full flowering due to the considerable amounts of rutin and epicatechin. alpha-Tocopherol content correlates positively with temperature, drought, and duration of solar radiation. Certain differences appear among varieties of buckwheat, especially in their squalene and rutin contents.
