Psoriasis and metabolic syndrome (MetS) are chronic inflammatory conditions associated with the dysregulation of immune system reactivity. The inflammatory processes of both diseases have not yet been fully characterized, and the evaluation of proteins/markers that could be involved in their pathogenesis is of great importance. We selected four markers: CRP, sCD200R1, CD5L, and sTLR2; in particular, sCDR2001 has not yet been measured in the context of psoriasis and metabolic syndrome. Material and methods: In the study, 64 controls and 43 patients with psoriasis with or without a metabolic syndrome were enrolled. The levels of selected markers were measured using ELISA kits. Results: CRP levels were significantly higher in psoriasis patients, especially in the subgroup of patients with MetS compared to nonMetS patients (p < 0.01). sCD200R1 and sTLR2 were not significantly different between groups and subgroups; however, CD200R1 levels were slightly higher in both control groups compared to both groups of patients. CD5L levels were significantly higher in patients with MetS compared to nonMets patients (p < 0.02). We also evaluated the correlations between parameters in controls and patients' groups, as well as in subgroups. Correlations between BMI and CRP were found in all groups and subgroups. Other correlations were group- and subgroup-specific. For example, in the patients' group, CD5L correlated with sCD200R1 (p < 0.05) and in MetS controls, with age (p < 0.03). Conclusion: The results show that the presence of systemic inflammation associated with psoriasis and metabolic syndrome and their combination alters the expression of specific molecules, especially CRP and CD5L, which were significantly increased in patients with psoriasis and a metabolic syndrome compared to controls without metabolic syndromes. Correlations between CRP and BMI in all groups suggest that overweight and obesity increase the intensity of inflammation and potentiate CD5L expression. In contrast, levels of molecules that may limit inflammation were not increased in psoriasis and metabolic syndrome subjects (they were non-significantly lower compared with healthy controls), which may reflect the chronic nature of both diseases and the exhaustion of inhibitory mechanisms.

• Publikační typ
• časopisecké články MeSH

Cíl studie: Stanovit hladiny solubilního Toll-like receptoru 2 (sTLR2) v pupečníkové krvi novorozenců pacientek s těhotenstvím komplikovaným předčasným odtokem plodové vody (PPROM) za přítomnosti nebo absence histologické chorioamnionitidy (HCA) a funisitidy. Typ studie: Retrospektivní studie. Název a sídlo pracoviště: Univerzita Karlova v Praze, Lékařská fakulta a Fakultní nemocnice v Hradci Králové, Ústav klinické imunologie a alergologie, Porodnická a gynekologická klinika. Metodika: Do studie bylo zařazeno 86 žen s PPROM mezi 24. a 36. týdnem gestačního stáří. Vzorky pupečníkové krve byly odebrány z podvázaného pupečníku ihned po porodu novorozence. Vzorky placenty, plodových obalů a pupečníku byly vyšetřeny na přítomnost zánětlivých změn. Koncentrace sTLR2 v pupečníkové krvi novorozenců byla měřena metodou ELISA. Výsledky: U žen s HCA nebyla zaznamenána rozdílná koncentrace sTLR2 v pupečníkové krvi ve srovnání s ženami bez HCA (s HCA: medián 7,6 ng/mL, interkvartilový rozsah [IQR] 5,1 – 12,3 vs. bez HCA: medián 8,0 ng/mL, IQR 6,0 – 9,4; p = 0,79). Nebyl nalezen rozdíl v koncentraci sTLR2 v pupečníkové krvi mezi skupinami pacientek s funisitidou a bez funisitidy (s funisitidou: medián 7,2 ng/mL,IQR 5,5 – 22,3 vs. bez funisitidy: medián 7,9 ng/mL, IQR 5,2 – 10,5; p = 0,31). Závěr: Hladiny sTLR2 v pupečníkové krvi nejsou ovlivněny přítomností HCA nebo funisitidy u těhotenství komplikovaných PPROM.

Objective: To determine whether umbilical cord blood concentrations of soluble Toll-like receptor (sTLR2) is of value in the diagnosis of histological chorioamnionitis (HCA) and funisitis in pregnancies complicated by preterm premature rupture of membranes. Design: Retrospective study. Setting: Charles University in Prague, Faculty of Medicine and University Hospital, Hradec Kralove, Department of Clinical Immunology and Allergy, Department of Obstetric and Gynecology. Methods: Eighty six women with PPROM between gestation ages 24 and 36 weeks were included in the study. The samples of the umbilical cord blood were taken from the clamped umbilical cord immediately after delivery of the newborn. The placenta, fetal membranes and umbilical cord were evaluated for the presence of inflammatory changes. The concentrations of sTLR2 in the umbilical cord blood were measured by ELISA method. Results: Women with HCA did not have different umbilical cord blood sTLR2 levels than women without HCA (with HCA: median 7.6 ng/mL, interquartile range [IQR] 5.1 – 12.3 vs. without HCA: median 8.0 ng/mL, IQR 6.0 – 9.4; p = 0.79). No differences between women with and without funisitis were found (median 7.2 ng/mL, IQR 5.5 – 22.3 vs. without funisitis: median 7.9 ng/mL, IQR 5.2 – 10.5; p = 0.31). Conclusion: Umbilical cord blood sTRL2 levels are not affected by the presence of either HCA or funisitis in pregnancies complicated with PPROM.

• MeSH
• biologické markery MeSH
• chorioamnionitida MeSH
• dospělí MeSH
• ELISA MeSH
• fetální krev * imunologie   MeSH
• hormony kůry nadledvin škodlivé účinky   MeSH
• infekční komplikace v těhotenství MeSH
• komplikace porodu MeSH
• komplikace těhotenství MeSH
• lidé MeSH
• předčasný odtok plodové vody * MeSH
• retrospektivní studie MeSH
• statistika jako téma MeSH
• těhotenství MeSH
• toll-like receptor 2 * fyziologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• práce podpořená grantem MeSH

OBJECTIVE: To determine the cervical and vaginal fluid soluble Toll-like receptor-2 (sTLR2) levels in pregnancies complicated by preterm prelabor rupture of membranes (PPROM) and their correlation to microbial invasion of the amniotic cavity (MIAC) and/or histological chorioamnionitis (HCA). METHODS: Sixty-eight women with singleton pregnancies complicated by PPROM were included in this study. Cervical and vaginal fluid was collected at the time of admission, and levels of sTLR2 in the cervical and vaginal fluid were determined using enzyme-linked immunosorbent assay. RESULT: Women with MIAC and both MIAC and HCA did not have different cervical and vaginal fluid sTLR2 levels compared to those without MIAC and without both MIAC and HCA. Women with HCA had higher cervical fluid sTLR2 levels in crude analysis (with HCA: median 11.6 pg/mL versus without HCA: median 5.5 pg/mL; p = 0.04) but not after adjustment for gestational age at sampling (p = 0.19). No difference in vaginal fluid sTLR2 levels between women with and without HCA was found. A positive correlation between cervical and vaginal fluid sTLR2 levels was identified (rho = 0.54; p < 0.0001). CONCLUSIONS: Cervical and vaginal fluid sTLR2 levels did not reflect the presence of MIAC and/or HCA.

• MeSH
• cervix uteri metabolismus   MeSH
• chorioamnionitida metabolismus   MeSH
• dospělí MeSH
• ELISA MeSH
• lidé MeSH
• novorozenec MeSH
• plodová voda mikrobiologie   MeSH
• předčasný odtok plodové vody metabolismus   patologie   MeSH
• těhotenství MeSH
• tělesné tekutiny metabolismus   MeSH
• toll-like receptor 2 metabolismus   MeSH
• vagina metabolismus   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• novorozenec MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

OBJECTIVE: To determine amniotic fluid soluble Toll-like receptor 2 (sTLR2) levels in PPROM according to the presence of microbial invasion of the amniotic cavity (MIAC), histological chorioamnionitis (HCA), and both these conditions. To test the cutoff level of 222.7 ng/mL, as proposed in our previous study, in order to distinguish women with both MIAC and HCA. METHODS: 169 women with a gestational age between 24+0 and 36+6 weeks were included in a prospective cohort study. Amniocenteses were performed, and sTLR2 in the amniotic fluid were determined using ELISA. RESULTS: Women with MIAC had higher sTLR2 levels (median 113.2 ng/mL) than those without MIAC (median 47.1 ng/mL; p < 0.0001). Women with HCA did not have a higher sTLR2 level (median 52.6 ng/mL) compared with women without HCA (median 47.1 ng/mL; p = 0.23). Women with both MIAC and HCA had higher sTLR2 levels (median: 311.3 ng/mL) than other women (17.5 ng/mL; p < 0.0001). The cutoff level 222.7 ng/mL had a sensitivity of 63%, a specificity of 98%, and a likelihood ratio of 40.3 for the prediction of both MIAC and HCA. CONCLUSIONS: Amniotic fluid sTLR2 is a promising predictor of both MIAC and HCA with high specificity in PPROM.

• MeSH
• amniocentéza MeSH
• amnion mikrobiologie   MeSH
• chorioamnionitida diagnóza   patologie   MeSH
• dospělí MeSH
• gestační stáří MeSH
• kohortové studie MeSH
• lidé MeSH
• novorozenec MeSH
• plodová voda chemie   mikrobiologie   MeSH
• předčasný odtok plodové vody metabolismus   mikrobiologie   MeSH
• prospektivní studie MeSH
• referenční hodnoty MeSH
• rozpustnost MeSH
• syndrom respirační tísně novorozenců diagnóza   MeSH
• těhotenství MeSH
• toll-like receptor 2 analýza   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• novorozenec MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

BACKGROUND: Toll-like receptor (TLR) 2 belongs to the large TLR receptor family comprised of at least 10 members with different roles in innate immunity. Psoriasis is recognized as a T-cell driven immune-mediated systemic inflammatory disease with a skin manifestation. An effective therapeutic approach to treat psoriasis is Goeckerman therapy (GT). The aim of this study was to assess both the kinetics of the expression of TLR2 on blood cells and the concentration of soluble (s)TLR2 in serum of patients with psoriasis and to examine the effect of GT on both TLR2 expression and sTLR2 level. METHODS: Both membrane and sTLR2 were determined in 20 patients and 20 healthy controls. sTLR2 was evaluated by enzyme-linked immunosorbent assay. Flow cytometry method was used to determine the expression of membrane TLR2 of monocytes and granulocytes. RESULTS: The serum level of sTLR2 was significantly lower (P < 0.0001) in patients both before and after GT compared to the control group. Compared to the membrane expression of TLR2 on monocytes of healthy blood donors, TLR2 expression was significantly higher in patients both before and after GT (P = 0.0001). Similarly, TLR2 expression on granulocytes was significantly higher in patients both before (P = 0.0061) and after (P < 0.0001) therapy than in control. CONCLUSIONS: Membrane and soluble TLR2 may be involved in the pathogenesis of psoriasis. Both remained unchanged by GT.

• MeSH
• buněčná membrána chemie   MeSH
• dehet uhelný terapeutické užití   MeSH
• dospělí MeSH
• fotochemoterapie * MeSH
• granulocyty chemie   MeSH
• keratolytika terapeutické užití   MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• monocyty chemie   MeSH
• psoriáza krev   farmakoterapie   MeSH
• studie případů a kontrol MeSH
• terapie ultrafialovými paprsky * MeSH
• toll-like receptor 2 analýza   krev   MeSH
• zdraví dobrovolníci pro lékařské studie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

OBJECTIVE: To determine soluble Toll-like receptor (sTLR) 1, sTLR2 and sTLR6 concentrations in amniotic fluid (AF) of women with preterm prelabor rupture of membranes (PPROM) and if there is an association with microbial invasion of the amniotic cavity and histological chorioamnionitis (HCA). METHODS: Cross-sectional study was performed. Forty-two women with singleton PPROM pregnancies at a gestational age between 24 + 0 and 36 + 6 weeks were included in the study (twenty-two women with presence of both microbial invasion of the amniotic cavity and HCA, and 20 women without microbial invasion of the amniotic cavity and HCA). Amniocenteses were performed, and the concentrations of sTLRs were determined by sandwich enzyme-linked immunosorbent assays. RESULTS: Women with microbial invasions of the amniotic cavity and HCA (n = 22) had significantly higher median sTLR1, sTLR2 and sTLR6 levels than those without (n = 20). (20.4 ng/mL vs. 0.44 ng/mL; p < 0.0001, 577.6 ng/mL vs. 60.7 ng/mL; p < 0.0001 and 0.44 ng/mL vs. 0.26 ng/mL; p = 0.02, respectively). CONCLUSIONS: Women with microbial invasion of the amniotic cavity and HCA had higher AF sTLR1, 2 and 6 levels.

• MeSH
• chorioamnionitida diagnóza   mikrobiologie   patologie   MeSH
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• morbidita MeSH
• multigenová rodina MeSH
• nemoci nedonošenců diagnóza   epidemiologie   MeSH
• novorozenec MeSH
• plodová voda chemie   metabolismus   mikrobiologie   MeSH
• předčasná porodní činnost diagnóza   epidemiologie   metabolismus   MeSH
• předčasný odtok plodové vody diagnóza   epidemiologie   metabolismus   MeSH
• prediktivní hodnota testů MeSH
• prenatální diagnóza metody   MeSH
• prognóza MeSH
• průřezové studie MeSH
• rozpustnost MeSH
• těhotenství MeSH
• toll-like receptor 1 analýza   genetika   metabolismus   fyziologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mladý dospělý MeSH
• novorozenec MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH