• MeSH
• chelátory MeSH
• cytochromy MeSH
• játra enzymologie   metabolismus   účinky léků   MeSH
• krysa rodu rattus MeSH
• xantheny MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH

• MeSH
• hypothalamus enzymologie   MeSH
• imunochemie MeSH
• krysa rodu rattus MeSH
• neurofysiny biosyntéza   metabolismus   MeSH
• oxidoreduktasy analýza   MeSH
• sulfhydrylové sloučeniny MeSH
• vasopresiny MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH

• Klíčová slova
• Cytembena,
• MeSH
• akryláty farmakologie   terapeutické užití   MeSH
• protinádorové látky farmakokinetika   farmakologie   MeSH
• sulfhydrylové sloučeniny chemie   MeSH

• MeSH
• antioxidancia MeSH
• biochemické jevy MeSH
• nemoci dýchací soustavy etiologie   MeSH
• oxidační stres MeSH
• reaktivní formy dusíku MeSH
• reaktivní formy kyslíku MeSH
• scavengery volných radikálů MeSH
• sloučeniny síry MeSH
• Publikační typ
• monografie MeSH

• Konspekt
• Farmacie. Farmakologie
• NLK Obory
• farmacie a farmakologie
• biochemie
• pneumologie a ftizeologie

• MeSH
• finanční podpora výzkumu jako téma MeSH
• kadmium toxicita   MeSH
• olovo toxicita   MeSH
• smrk účinky léků   MeSH
• sulfhydrylové sloučeniny metabolismus   MeSH

• MeSH
• biologická adaptace fyziologie   účinky léků   MeSH
• cisplatina farmakologie   toxicita   MeSH
• finanční podpora výzkumu jako téma MeSH
• fyziologie rostlin imunologie   MeSH
• látky znečišťující životní prostředí farmakologie   toxicita   MeSH
• rostliny chemie   účinky léků   MeSH
• sulfhydrylové sloučeniny MeSH

• MeSH
• antibakteriální látky MeSH
• glutathion MeSH
• houby MeSH
• inhibitory enzymů MeSH
• lékové interakce MeSH
• spektrofotometrie MeSH
• sulfhydrylové sloučeniny MeSH

OBJECTIVES: The aim of this study was to assess the effect of metals, with special attention of cadmium (Cd), content of the Svitava and Svratka rivers (Czech Republic) on levels of thiol compounds such as metallothioneins (MT), glutathione (GSH/GSSG), and cysteine (Cys) concentrations in chub (Leuciscus cephalus L.) liver and their use as biomarkers. DESIGN: Levels of thiol compounds in fish liver were determined by the differential pulse voltammetry Brdicka reaction. Cadmium content in L. cephalus liver was measured by atomic absorption spectometry (AAS) with electrothermal atomization. The contents of thiols and Cd were correlated with the Cd and metals content of river bottom sediments. RESULTS: Results were inconsistent with those of experimental studies. Correlations between liver Cd content and MT, Cys, GSSG were not significant in any locality (p > 0.05). Cd liver content was negatively (p< 0.05) correlated with GSH at one (Zidlochovice) of the seven tested sites. GSH and GSSG correlated negatively in five localities (p > 0.05). Correlations between Cd in sediment and Cys and GSSG (p > 0.05), and MT was negative (p< 0.05). The relationship between sum of metals value and MT was negative (p< 0.05), similarly with GSSG (p > 0.05). CONCLUSION: Many factors influence content of MT, GSH, and GSSG, and data for Cd were equivocal. Monitoring the aquatic ecosystem to identify metal content using thiol compounds in fish liver as biomarkers is complex. More relevant data including laboratory experiments will be required to assess most of the factors influencing thiols and their relevance to Cd contamination.

• MeSH
• biologické markery analýza   metabolismus   MeSH
• Cyprinidae metabolismus   MeSH
• cystein metabolismus   MeSH
• glutathion metabolismus   MeSH
• játra chemie   metabolismus   MeSH
• kadmium analýza   metabolismus   MeSH
• kovy analýza   metabolismus   MeSH
• řeky chemie   MeSH
• spektrofotometrie atomová MeSH
• sulfhydrylové sloučeniny analýza   metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH

• MeSH
• anestetika lokální MeSH
• antiarytmika MeSH
• glukuronidasa MeSH
• koronární nemoc patofyziologie   MeSH
• krysa rodu rattus MeSH
• myokard MeSH
• srdce účinky léků   MeSH
• sulfhydrylové sloučeniny MeSH
• Check Tag
• krysa rodu rattus MeSH

BACKGROUND: Fibromyalgia syndrome (FMS) is an extra-articular rheumatological disease, characterized by widespread pain and somatic symptoms. The etiology has not yet been clarified. Oxidative stress may play an important role in FMS etiology. Thiol group is a very strong antioxidant. We aimed to investigate whether thiol/disulfide homeostasis in FMS is altered or not. MATERIAL AND METHODS: A total of 80 female FMS patients and 64 healthy female control individuals were included in this study. Thiol and disulfide values were measured by Erel's novel methods. RESULTS: Native thiol (330.6 ± 46.1 vs. 356.8 ± 55.5 μmol/L, p = 0.005) and native thiol/total thiol (89.4 ± 3.2 vs. 93.3 ± 4.0, p < 0.001) levels of FMS patients were significantly lower when compared to the values of control group. However, disulfide (19.4 ± 6.3 vs. 12.2 ± 6.3 μmol/L, p < 0.001) levels of FMS patients were significantly higher than healthy individuals. A negative correlation was found between the native thiol/total thiol and fibromyalgia impact questionnaire (FIQ) score among the FMS patients. A positive correlation was found between disulfide values and FIQ score among the patients. CONCLUSIONS: In FMS patients, there was a significant correlation between the decrease in the thiol levels and an increase in the disulfide levels with the FIQ scores. We determined that thiol-disulfide rate was deteriorated in FMS patients and it increases in favor of disulfide amounts.

• MeSH
• antioxidancia metabolismus   MeSH
• biologické markery krev   MeSH
• disulfidy krev   MeSH
• dospělí MeSH
• fibromyalgie krev   patofyziologie   MeSH
• homeostáza * MeSH
• lidé středního věku MeSH
• lidé MeSH
• oxidační stres fyziologie   MeSH
• průřezové studie MeSH
• sulfhydrylové sloučeniny krev   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH