thyme Dotaz Zobrazit nápovědu
- MeSH
- antibakteriální látky * farmakologie MeSH
- Candida tropicalis účinky léků MeSH
- Escherichia coli účinky léků MeSH
- mikrobiální testy citlivosti metody MeSH
- Penicillium chrysogenum účinky léků MeSH
- potravinářská mikrobiologie MeSH
- rostlinné extrakty * MeSH
- techniky in vitro MeSH
- těkavé organické sloučeniny * farmakologie MeSH
- Thymus (rostlina) chemie MeSH
- Publikační typ
- práce podpořená grantem MeSH
Meat is a high protein meal that is used in the preparation of jerky, a popular food snack, where preservation and safety are important. To assure food safety and to extend the shelf life of meat and meat products, the use of either synthetic or natural preservatives have been applied to control and eliminate foodborne bacteria. A growing interest in the application of natural food additives for meat has increased. Microorganisms, such as Escherichia coli, contaminate meat and meat products, causing foodborne illnesses. Therefore, it is necessary to improve the meat conservation process. However, the use of essential oils when the meat is being dried has not been deeply studied. In this regard, there is an opportunity to increase the value of dried meat and reduce the risk of foodborne illnesses by applying essential oils during the drying process. In this protocol, we present a novel method of applying thyme essential oil (TEO) during meat drying, specifically in vapor form directly in a drying chamber. For evaluation, we use Minimal Inhibitory Concentration (MIC) to detect the number of harmful bacteria in the treated samples compared to raw samples. The preliminary results show that this method is a viable and alternative option to synthetic preservatives and that it significantly reduces microbial load in dried meat.
- MeSH
- manipulace s potravinami metody MeSH
- masné výrobky mikrobiologie MeSH
- oleje prchavé farmakologie MeSH
- potraviny konzervované mikrobiologie MeSH
- skot MeSH
- Thymus (rostlina) chemie MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- audiovizuální média MeSH
- časopisecké články MeSH
- práce podpořená grantem MeSH
In this study we analyzed molecular mechanisms of antioxidative protection of thymol and thyme oil using differentiated PC12 cells, a widely accepted neuronal model. Thymol due to multiple functions is commonly used for clinical applications. However, its action on nervous tissue remains poorly understood. We evaluated the effect of 24 h incubation of the cells with thymol (100 and 400 μM) and equivalent content of thyme oil. Microsomal glutathione transferase 1 (Mgst1) is an important player in anti-oxidative protection because of its transferase and peroxidase activities. Since its expression decreases during aging, we also used stable transfected cells with downregulated Mgst1 (PC12_M). We analyzed cell viability, lipid peroxidation level, glutathione content and expression of key enzymes responsible for cellular reduced glutathione – catalytic subunit of γ-glutamylcysteine ligase and glutathione reductase. Whereas thymol and thyme oil improved antioxidant capacity of control cells, diminished protection was observed in PC12_M line. Increasing interest in natural dietary components has focused attention on plants used as a rich source of bioactive phytochemicals. However, currently available information on the safe amount for thyme oil using appears to be insufficient. Our results indicate that the thyme oil should be used with caution, especially by elderly people.
- MeSH
- antioxidancia * fyziologie chemie MeSH
- buňky PC12 účinky léků MeSH
- glutathion fyziologie účinky léků MeSH
- glutathiontransferasa MeSH
- neurony účinky léků MeSH
- oleje rostlin * farmakologie chemie MeSH
- peroxidace lipidů účinky léků MeSH
- stárnutí účinky léků MeSH
- statistika jako téma MeSH
- techniky in vitro statistika a číselné údaje MeSH
- thymol * farmakologie MeSH
- Thymus (rostlina) MeSH
- viabilita buněk účinky léků MeSH
- Publikační typ
- hodnotící studie MeSH
- práce podpořená grantem MeSH
BACKGROUND: A comparative analysis by using static headspace (HS) and steam distillation (SD) GC-MS of the volatile and the semi-volatile secondary metabolites from leaves of cultivated Majorana syriaca. METHODS: The essential oils endogenous to cultivated thyme were isolated and identified by HS-GC-MS technology and compared to those from SD-GC-MS. RESULTS: The HS-GC-MS results showed that the Palestinian cultivated thyme is rich in monoterpene hydrocarbons and phenolic monoterpenes such as alpha-phellandrene, alpha-pinene, beta-myrcene, m-cymene, p-cymene, gamma-terpinene, thymol and carvacrol. In all the samples gamma-terpinene, p-cymene, thymol and carvacrol were the most abundant compounds. CONCLUSIONS: HS and SD-GC-MS have proved that most of the cultivated thyme samples examined has thymol isomer as the major phenolic constituent.
- MeSH
- dobromysl (rod) chemie MeSH
- listy rostlin chemie MeSH
- monoterpeny analýza MeSH
- plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
- thymol analýza MeSH
- Thymus (rostlina) chemie MeSH
- Publikační typ
- časopisecké články MeSH
- srovnávací studie MeSH
- Geografické názvy
- Střední východ MeSH
Léčivé rostliny jsou s výhodou používány v terapii onemocnění cest dýchacích. Katary horních cest dýchacích patří mezi nemoci spojené se sezónním snížením imunity, proto je často používáno rostlinných drog s nespecifickým imunomodulačním účinkem. Mezi takové rostliny patří např. echinacea (Echinacea purpurea) a americký ženšen (Panax quinquefolius). Ve spojení s antibakteriálně a antisepticky účinnými léčivými rostlinami, jako jsou tymián (Thymus vulgaris) a pelargonium (Pelargonium sidoides) mohou představovat účinnou pomoc při onemocněních cest dýchacích, zkracovat trvání nemoci a snižovat nutnost antibiotické terapie. Prezentovaný text shrnuje základní informace o těchto rostlinách, jejich obsahových látkách, mechanismech účinku a klinických zkouškách potvrzujících efekt a monitorujících případné nežádoucí účinky.
Medicinal plants are advantageously used in the treatment of respiratory tract diseases. Upper respiratory tract catarrh is one of the diseases associated with seasonal weakening of immunity, and therefore, plant drugs with a non-specific immunomodulation effect are often used. Such plants include, but are not limited to, Echinacea (Echinacea purpurea) and American ginseng (Panax quinquefolius). In combination with medicinal plants having antibacterial and antiseptic effects, such as thyme (Thymus vulgaris) and pelargonium (Pelargonium sidoides), they can constitute efficient help in the treatment of respiratory tract diseases, shorten the duration of the disease and reduce the need of antibiotic therapy. The text presented summarizes the basic information about these plants, their ingredients, mechanisms of action and clinical tests confirming their effect and monitoring eventual adverse effects.
