Porcine thymus contains three independent populations of cells that have rearranged immunoglobulin heavy chain VDJH genes. The first population can be found exclusively in medulla and it consists of existing mature B cells and plasma cells. The second consists of developing B cells characterized by the presence of selected VDJH rearrangement, similar to B cell lymphogenesis in the bone marrow. The third population is entirely unaffected by selection mechanism for productive VDJH rearrangement and represents T lineage cells that rearrange immunoglobulin genes. Transcription of unselected VDJH repertoire is not allowed in T cells. Sequence analysis of unselected VDJH repertoire from T cells also revealed important consequences for B cell lymphogenesis and selection of B cell repertoire. As far as we know, this is the first evidence that some species completely rearrange VDJH genes in T cells. Our results also support the finding that B cells actively develop in the thymus.

• MeSH
• B-lymfocyty imunologie   MeSH
• druhová specificita MeSH
• geny pro těžké řetězce imunoglobulinů genetika   MeSH
• lidé MeSH
• plod imunologie   MeSH
• podskupiny lymfocytů imunologie   MeSH
• prasata genetika   růst a vývoj   imunologie   MeSH
• T-lymfocyty imunologie   MeSH
• thymus růst a vývoj   imunologie   MeSH
• V(D)J rekombinace genetika   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

The adaptive immune system of higher vertebrates is believed to have evolved to counter the ability of pathogens to avoid expulsion because their high rate of germline mutations. Vertebrates developed this adaptive immune response through the evolution of lymphocytes capable of somatic generation of a diverse repertoire of their antigenic receptors without the need to increase the frequency of germline mutation. The focus of our research and this article is on the ontogenetic development of the lymphocytes, and the repertoires they generate in swine. Several features are discussed including (a) the "closed" porcine placenta means that de novo fetal development can be studied for 114 days without passive influence from the mother, (b) newborn piglets are precocial permitting them to be reared without their mothers in germ-free isolators, (c) swine are members of the γδ-high group of mammals and thus provides a greater opportunity to characterize the role of γδ T cells and (d) because swine have a simplified variable heavy and light chain genome they offer a convenient system to study antibody repertoire development.

• MeSH
• adaptivní imunita * MeSH
• B-lymfocyty imunologie   MeSH
• gastrointestinální trakt imunologie   MeSH
• genetická variace MeSH
• imunogenetické jevy MeSH
• imunoglobuliny genetika   MeSH
• lidé MeSH
• lymfoidní progenitorové buňky fyziologie   MeSH
• modely u zvířat MeSH
• prasata MeSH
• receptory antigenů genetika   MeSH
• sekvence nukleotidů MeSH
• T-lymfocyty imunologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH