Patch clamp method developed more than 30 years ago is widely used for investigation of cellular excitability manifested as transmembrane ionic current and/or generation of action potentials. This technique could be applied to measurement of ionic currents flowing through individual (single) ion channels or through the whole assembly of ion channels expressed in the whole cell. Whole cell configuration is more common for measurement of ion currents and the only one enabling measurement of action potentials. This method allows detailed analysis of mechanisms and structural determinants of voltagedependent gating of ion channels as well as regulation of channel activity by intracellular signaling pathways and pharmacological agents.

• MeSH
• akční potenciály MeSH
• biologické modely MeSH
• buněčná membrána metabolismus   MeSH
• financování organizované MeSH
• gating iontového kanálu MeSH
• iontové kanály metabolismus   MeSH
• lidé MeSH
• membránové potenciály MeSH
• metoda terčíkového zámku MeSH
• vápníková signalizace MeSH
• vápníkové kanály - typ T metabolismus   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• přehledy MeSH