Endoglin (a type III TGF-β receptor) is able to modulate ligand binding and signaling by association with the TGF-β type I receptors (ALK-1 and ALK-5). In this study, we hypothesized whether atorvastatin treatment affects endoglin/ALK-1/p-Smad1/VEGF expression in the aorta and endoglin levels in serum in ApoE/LDLR double knockout mice. ApoE/LDLR double knockout mice were fed with a diet containing either 1% of cholesterol (CHOL) or cholesterol with atorvastatin (ATV) at a dose of 50mg/kg/day. Biochemical analysis of cholesterol levels and ELISA analysis of endoglin levels in serum, lesion area size, immunohistochemistry and Western blot analysis in mice aorta were performed. Atorvastatin treatment resulted in a significant decrease of total, VLDL and LDL cholesterol, atherosclerotic lesion size and endoglin serum levels in comparison with CHOL mice. On the other hand, atorvastatin treatment significantly increased the expressions of endoglin by 1431%, ALK-1 by 310%, p-Smad1 by 135% and VEGF by 62% in aorta when compared to CHOL mice. In conclusion, it has been demonstrated that atorvastatin increases endoglin/ALK-1/p-Smad1/VEGF expression in aorta and decreases the size of atherosclerotic lesions, suggesting that activation of this endothelial-protective pathway might support the antiatherogenic effects of atorvastatin. Moreover, atorvastatin concurrently decreased serum levels of endoglin suggesting that monitoring of endoglin levels in blood might represent an important marker of the progression and/or treatment of atherosclerosis.

• MeSH
• aktivinové receptory typu I metabolismus   MeSH
• apolipoproteiny E genetika   MeSH
• aterosklerotický plát chemicky indukované   metabolismus   patologie   prevence a kontrola   MeSH
• ateroskleróza krev   chemicky indukované   metabolismus   patologie   prevence a kontrola   MeSH
• biomarkery farmakologické krev   metabolismus   MeSH
• cholesterol dietní farmakologie   MeSH
• cholesterol krev   MeSH
• fosforylace účinky léků   MeSH
• HDL-cholesterol krev   MeSH
• intracelulární signální peptidy a proteiny krev   metabolismus   MeSH
• krev účinky léků   MeSH
• kyseliny heptylové farmakologie   terapeutické užití   MeSH
• LDL-cholesterol krev   MeSH
• myši inbrední C57BL MeSH
• myši knockoutované MeSH
• myši MeSH
• protein Smad1 metabolismus   MeSH
• pyrroly farmakologie   terapeutické užití   MeSH
• receptory LDL genetika   MeSH
• Valsalvův sinus metabolismus   patologie   MeSH
• vaskulární endoteliální růstový faktor A metabolismus   MeSH
• VLDL-cholesterol krev   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH