• MeSH
• antigeny CD3 analýza   MeSH
• Bacillus imunologie   MeSH
• cytologické techniky MeSH
• imunizace MeSH
• imunoglobuliny analýza   MeSH
• nádory imunologie   terapie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• srovnávací studie MeSH

• MeSH
• aktivace makrofágů MeSH
• Bacillus imunologie   MeSH
• buněčné kultury MeSH
• cytokiny imunologie   metabolismus   MeSH
• finanční podpora výzkumu jako téma MeSH
• myši MeSH
• oxid dusnatý metabolismus   MeSH
• peritoneální dutina cytologie   MeSH
• přirozená imunita MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH

Mucosal immunization with non-living antigens usually requires the use of an adjuvant. The adjuvant activity of Bacillus firmus in the mucosal immunization of mice was described by our laboratory previously. In the present study, subcellular localization of B. firmus activities was followed. After mechanical disintegration, subcellular components of bacterium were fractionated by differential centrifugation and salting out. Bacterial cell walls, cytoplasmic membrane fraction, soluble cytoplasmic proteins, and ribosomal fractions were isolated. Their effect on the mouse immune system was studied. Lymphocyte proliferation and immunoglobulin formation in vitro were stimulated by bacterial cell wall (BCW), cytoplasmic membrane (CMF), and ribosomal fractions. BCW and CMF increased antibody formation after intratracheal immunization of mice with influenza A and B viruses, and increased protection against subsequent infection with influenza virus. The BCW fraction even induced intersubtypic cross-protection: Mice immunized with A/California/7/04 (H3N2) + BCW were resistant to the infection by the highly pathogenic A/PR/8/34 (H1N1) virus.

• MeSH
• adjuvancia imunologická aplikace a dávkování   izolace a purifikace   MeSH
• Bacillus chemie   MeSH
• infekce viry z čeledi Orthomyxoviridae prevence a kontrola   MeSH
• kultivované buňky MeSH
• lymfocyty účinky léků   MeSH
• modely nemocí na zvířatech MeSH
• myši MeSH
• proliferace buněk účinky léků   MeSH
• tvorba protilátek účinky léků   MeSH
• vakcíny proti chřipce aplikace a dávkování   MeSH
• virus chřipky A imunologie   MeSH
• virus chřipky B imunologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Maternal milk has beneficial effects on the development and function of the newborn's immune system. Whether the milk of allergic mother has the same effects as the milk of healthy mothers is not yet quite clear. To contribute to the characterization of its immunomodulatory action, we tested the effect of milk of healthy and allergic mothers on the proliferation and immunoglobulin formation in cultures of cord blood mononuclear leucocytes (CBML) of newborns of healthy and allergic mothers. CBML proliferation was tested by (3)H-thymidine incorporation, IgM, IgG and IgA production by reverse ELISPOT. CBML response was examined in unstimulated cultures and after stimulation with polyclonal activators in the presence or absence of colostrum or milk. The cells of children of allergic mothers have a significantly higher proliferative activity than those of children of healthy mothers. Maternal colostrum/milk in high doses markedly suppresses cell proliferation after stimulation with polyclonal activators, whereas lower milk doses in the cultures have no such effect and exert a rather stimulatory action. Immunoglobulin production by cord blood lymphocytes is also different in the two groups of children. Low basal immunoglobulin formation is increased after stimulation with a strong polyclonal activator of B cells--Bacillus firmus, CBML of children of allergic mothers produce more IgA than those of children of healthy mothers. The stimulated production of all immunoglobulin classes in cells of children of healthy mothers is still enhanced by colostrum/milk. Children of allergic mothers show a markedly increased production of only IgM and IgA. The effect of healthy and allergic colostrum and milk on cell proliferation and immunoglobulin production is similar. The lymphocytes of children of allergic mothers differ from the lymphocytes of children of healthy mothers in their proliferative activity and the ability to form immunoglobulin already at birth.

• MeSH
• aktivace lymfocytů MeSH
• alergie imunologie   metabolismus   MeSH
• fetální krev cytologie   imunologie   metabolismus   MeSH
• financování organizované MeSH
• imunoglobulin A metabolismus   MeSH
• imunoglobulin G metabolismus   MeSH
• imunoglobulin M metabolismus   MeSH
• imunologická odpověď na dávku MeSH
• kojení MeSH
• kolostrum imunologie   MeSH
• kultivované buňky MeSH
• lidé MeSH
• lymfocyty imunologie   metabolismus   MeSH
• mateřské mléko imunologie   MeSH
• nemoci novorozenců imunologie   metabolismus   MeSH
• novorozenec MeSH
• proliferace buněk MeSH
• studie případů a kontrol MeSH
• těhotenství MeSH
• Check Tag
• lidé MeSH
• novorozenec MeSH
• těhotenství MeSH
• ženské pohlaví MeSH