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Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus
D. Cechova, M. Novakova, K. Mikulik, O. Novotna, J. Julak, P. Zanvit, L. Prokesova
Jazyk angličtina Země Česko
Typ dokumentu časopisecké články, práce podpořená grantem
- MeSH
- adjuvancia imunologická aplikace a dávkování izolace a purifikace MeSH
- Bacillus chemie MeSH
- infekce viry z čeledi Orthomyxoviridae prevence a kontrola MeSH
- kultivované buňky MeSH
- lymfocyty účinky léků MeSH
- modely nemocí na zvířatech MeSH
- myši MeSH
- proliferace buněk účinky léků MeSH
- tvorba protilátek účinky léků MeSH
- vakcíny proti chřipce aplikace a dávkování MeSH
- virus chřipky A imunologie MeSH
- virus chřipky B imunologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Mucosal immunization with non-living antigens usually requires the use of an adjuvant. The adjuvant activity of Bacillus firmus in the mucosal immunization of mice was described by our laboratory previously. In the present study, subcellular localization of B. firmus activities was followed. After mechanical disintegration, subcellular components of bacterium were fractionated by differential centrifugation and salting out. Bacterial cell walls, cytoplasmic membrane fraction, soluble cytoplasmic proteins, and ribosomal fractions were isolated. Their effect on the mouse immune system was studied. Lymphocyte proliferation and immunoglobulin formation in vitro were stimulated by bacterial cell wall (BCW), cytoplasmic membrane (CMF), and ribosomal fractions. BCW and CMF increased antibody formation after intratracheal immunization of mice with influenza A and B viruses, and increased protection against subsequent infection with influenza virus. The BCW fraction even induced intersubtypic cross-protection: Mice immunized with A/California/7/04 (H3N2) + BCW were resistant to the infection by the highly pathogenic A/PR/8/34 (H1N1) virus.
Citace poskytuje Crossref.org
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