Humoral immunity in mammals relies on the function of two developmentally and functionally distinct B-cell subsets-B1 and B2 cells. While B2 cells are responsible for the adaptive response to environmental antigens, B1 cells regulate the production of polyreactive and low-affinity antibodies for innate humoral immunity. The molecular mechanism of B-cell specification into different subsets is understudied. In this study, we identified lysine methyltransferase NSD2 (MMSET/WHSC1) as a critical regulator of B1 cell development. In contrast to its minor impact on B2 cells, deletion of the catalytic domain of NSD2 in primary B cells impairs the generation of B1 lineage. Thus, NSD2, a histone H3 K36 dimethylase, is the first-in-class epigenetic regulator of a B-cell lineage in mice.

• MeSH
• analýza přežití MeSH
• B-lymfocyty metabolismus   MeSH
• histonlysin-N-methyltransferasa chemie   metabolismus   MeSH
• histony metabolismus   MeSH
• humorální imunita MeSH
• katalytická doména * MeSH
• lysin metabolismus   MeSH
• metylace MeSH
• myši inbrední C57BL MeSH
• novorozená zvířata MeSH
• přesmyk imunoglobulinových tříd MeSH
• vztahy mezi strukturou a aktivitou MeSH
• zárodečné centrum lymfatické uzliny metabolismus   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH