Non-tuberculous mycobacteria are increasingly described as infectious agents in immunocompromised patients. A 17-year-old male patient suffering from secondary non-Hodgkin's lymphoma and treated with chemotherapeutic agents was admitted to hospital due to pleuropneumonia. Mycobacterium neoaurum was cultured repeatedly from his sputum and, Mycobacterium avium subsp. avium (M. a. avium) was detected by IS901 qPCR from detached fragments of his intestinal mucosa. We attempted to determine the possible sources of infection by analysing environmental samples from the closed oncology unit and conventional unit in the hospital, and from the patient's home residence and places which he frequented. The environment of the patient harboured mycobacteria (41 isolates in total); however, M. neoaurum was not recovered. M. a. avium was detected by qPCR in the environmental samples from a small flock of hens kept by his neighbour. Although it was not confirmed by DNA fingerprinting methods, the M. a. avium infection could have been acquired through the eating of incompletely cooked eggs.
- MeSH
- antituberkulotika terapeutické užití MeSH
- antitumorózní látky terapeutické užití MeSH
- atypické mykobakteriální infekce * MeSH
- ciprofloxacin terapeutické užití MeSH
- hostitel s imunodeficiencí * MeSH
- lidé MeSH
- mikrobiologie životního prostředí MeSH
- mladiství MeSH
- Mycobacterium avium izolace a purifikace MeSH
- nehodgkinský lymfom farmakoterapie MeSH
- netuberkulózní mykobakterie izolace a purifikace MeSH
- sputum mikrobiologie MeSH
- střevní sliznice mikrobiologie MeSH
- tuberkulóza * MeSH
- Check Tag
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- práce podpořená grantem MeSH
The environment is a reservoir of nontuberculous mycobacteria and is considered a source of infection for animals and humans. Mycobacteria can persist in different types of environments for a relatively long time. We have studied their possible internalization into plant tissue through intact, as well as damaged, root systems of different types of plants grown in vitro and under field conditions. The substrate into which plants were seeded was previously contaminated with different strains of Mycobacterium avium (10(8) to 10(10) cells/g of soil) and feces from animals with paratuberculosis. We detected M. avium subsp. avium, hominissuis, and paratuberculosis in the stems and leaves of the plants by both culture and real-time quantitative PCR. The presence of mycobacteria in the plant tissues was confirmed by microscopy. The concentration of mycobacteria found inside plant tissue was several orders of magnitude lower (up to 10(4) cells/g of tissue) than the initial concentration of mycobacteria present in the culture medium or substrate. These findings led us to the hypothesis that plants may play a role in the spread and transmission of mycobacteria to other organisms in the environment.
- MeSH
- bakteriologické techniky MeSH
- endocytóza * MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- listy rostlin mikrobiologie MeSH
- mikroskopie MeSH
- Mycobacterium genetika růst a vývoj fyziologie MeSH
- rostliny mikrobiologie MeSH
- stonky rostlin mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Mycobacterium avium subsp. avium (MAA) and M. a. hominissuis (MAH) belong to the Mycobacterium avium complex (MAC) and are frequently associated with diseases in animals and humans. The aim of this study was to develop a system for rapid and accurate real time quantitative PCR (qPCR) identification and quantification of MAA and MAH. This study included 22 per os infected pigs, of which 10 were infected with MAA, 10 with MAH and 2 were present as a negative control group. From each animal, 21 different tissue samples as well as blood were tested by microscopy, culture and triplex qPCR. The developed triplex qPCR reaction was based on the simultaneous detection of specific insertion sequences, IS901 and IS1245, and also contained an internal amplification control. In both groups of experimentally infected animals, the newly developed triplex qPCR assay proved to be more specific and sensitive in comparison with the other methods used. Contrary to culture examination, triplex qPCR confirmed the infection in all animals infected with MAA, and in eight animals infected with MAH. In conclusion, we developed a quick and sufficiently sensitive triplex qPCR for MAA and MAH detection in tissue and blood samples. From the food safety point of view the presence of MAH in muscles should be considered as a possible threat to human health.
- MeSH
- gastrointestinální trakt mikrobiologie MeSH
- játra mikrobiologie MeSH
- lidé MeSH
- lymfatické uzliny mikrobiologie MeSH
- Mycobacterium avium komplex MeSH
- nemoci prasat mikrobiologie MeSH
- polymerázová řetězová reakce veterinární MeSH
- prasata MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- tuberkulóza mikrobiologie veterinární MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Publikační typ
- abstrakt z konference MeSH
