- MeSH
- diagnostické zobrazování MeSH
- dospělí MeSH
- Echinococcus granulosus patogenita účinky léků MeSH
- infekce virem Epsteina-Barrové diagnóza terapie MeSH
- lidé MeSH
- lymfatické nemoci diagnostické zobrazování diagnóza mikrobiologie terapie MeSH
- lymfatické uzliny * diagnostické zobrazování mikrobiologie patologie MeSH
- termografie MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Salmonella Typhimurium is an enteric pathogen that causes acute and chronic infections in humans and animals. One-week-old germ-free piglets were orally colonized/infected with the Salmonella Typhimurium LT2 strain or its isogenic rough ΔrfaL, ΔrfaG or ΔrfaC mutants with exactly defined lipopolysaccharide (LPS) defects. After 24 h, the piglets were euthanized and the colonization of the small intestine, translocations into the mesenteric lymph nodes, liver, spleen, lungs, and bacteremia, along with changes in the ileum histology, and transcription levels of the tight junction proteins claudin-1, claudin-2, and occludin were all assessed. Additionally, transcription levels of IL-8, TNF-α, and IL-10 in the terminal ileum, and their local and systemic protein levels were evaluated. Wild-type Salmonella Typhimurium showed the highest translocation, histopathological changes, upregulation of claudins and downregulation of occludin, transcription of the cytokines, intestinal IL-8 and TNF-α levels, and systemic TNF-α and IL-10 levels. Depending on the extent of the incompleteness of the LPS, the levels of the respective elements decreased, or no changes were observed at all in the piglets colonized/infected with Δrfa mutants. Intestinal IL-10 and systemic IL-8 levels were not detected in any piglet groups. This study provided foundational data on the gnotobiotic piglet response to colonization/infection with the exactly defined rough Salmonella Typhimurium LT2 isogenic mutants.
- MeSH
- cytokiny imunologie MeSH
- gnotobiologické modely * MeSH
- játra mikrobiologie MeSH
- lipopolysacharidy toxicita MeSH
- lymfatické uzliny mikrobiologie MeSH
- mutace MeSH
- plíce mikrobiologie MeSH
- prasata MeSH
- Salmonella typhimurium genetika fyziologie MeSH
- salmonelóza imunologie mikrobiologie patologie MeSH
- slezina mikrobiologie MeSH
- tenké střevo imunologie mikrobiologie patologie MeSH
- virulence * MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A total of 156 tonsils and 156 mandibular lymph nodes from fattening pigs originating from 13 farms were sampled in Croatian slaughterhouses and examined for Salmonella spp. (n=78 per organ) and Yersinia enterocolitica (n=78 per organ) by cultural methods. Salmonella was isolated from two tonsils only, both originated from animals from the same farm (5.12%), while Y. enterocolitica were recovered from 26 tonsils (33.33%) which could be traced back to 10 farms. Salmonella was absent in mandibular lymph nodes, and Y. enterocolitica was isolated from eight lymph nodes (10.25%) which originated from six farms. Y. enterocolitica was present inside the lymph nodes of two pigs. The high prevalence of Y. enterocolitica in/on pig tonsils could be the result of cross-contamination during splitting the carcasses with head. This procedure may result in higher prevalence of Y. enterocolitica on surface of mandibular lymph nodes than in their depth. Traditional veterinary postmortem examination of pig halves will not necessarily contribute to cross-contamination with Salmonella or Yersinia under conditions of present slaughter practice.
- MeSH
- bakteriologické techniky MeSH
- infekce yersiniemi epidemiologie mikrobiologie veterinární MeSH
- jatka MeSH
- krční mandle mikrobiologie MeSH
- lymfatické uzliny mikrobiologie MeSH
- mrtvola MeSH
- prasata MeSH
- prevalence MeSH
- Salmonella izolace a purifikace MeSH
- salmonelová infekce u zvířat epidemiologie mikrobiologie MeSH
- Yersinia enterocolitica izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Chorvatsko MeSH
Nontuberculous mycobacteria (NTM) are ubiquitous, potentially pathogenic organisms that have been isolated from a variety of environmental sources. NTM have been isolated from various kinds of food and many studies support the hypothesis that food, especially raw or partially cooked products, plays a role as a source of NTM for humans. Animals with disseminated infection have been diagnosed with NTM not only in the gastro-intestinal tract and intestinal lymph nodes, but also in tissues like muscle and parenchymatous organs. Infected animals may harbor NTM in their tissues even without clinical symptoms and especially minced meat with the possible addition of lymph nodes are considered as potential source of NTM. The purpose of this paper was to review articles concerning the detection of mycobacteria in the muscle tissue and lymph nodes of domestic animals, farmed and free-living game and to summarize methods and techniques for their detection.
- MeSH
- divoká zvířata mikrobiologie MeSH
- hospodářská zvířata mikrobiologie MeSH
- kosterní svaly mikrobiologie MeSH
- lymfatické uzliny mikrobiologie MeSH
- masné výrobky mikrobiologie MeSH
- Mycobacterium avium růst a vývoj izolace a purifikace MeSH
- netuberkulózní mykobakterie růst a vývoj izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
This study was performed on 40 finished pigs from one herd naturally infected with Mycobacterium avium subsp. avium. The aim was to investigate the presence and amount of M. a. avium in samples of lymph nodes and diaphragm tissues collected during routine postmortem inspection using the triplex quantitative real time PCR (qPCR) method. We collected, in total, 107 samples: various lymph nodes affected by gross tuberculosis (TB)-like lesions from 17 pig carcasses, as well as samples of head and mesenteric lymph nodes from 23 carcasses without TB-like lesions. Samples of diaphragm tissues were collected from all carcasses. M. a. avium was detected in one or more tissue samples collected from half of the slaughtered pigs tested. Samples of diaphragm tissues of three pigs with detected TB-like lesions contained M. a. avium (10(2) to 10(3) cells per g of sample); the organism was not detected in diaphragm tissues from pigs without TB-like lesions. The qPCR method may be useful for quantification of M. a. avium in pigs for the purposes of foodborne risk assessment.
- MeSH
- bezpečnost potravin MeSH
- bránice mikrobiologie patologie MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lymfatické uzliny mikrobiologie patologie MeSH
- Mycobacterium avium izolace a purifikace MeSH
- nemoci prasat mikrobiologie patologie MeSH
- prasata MeSH
- tuberkulóza mikrobiologie patologie veterinární MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika MeSH
Lymfangioleiomyomatóza (LAM) je zriedkavé ochorenie vyskytujúce sa u žien v reprodukčnom veku. LAM je charakterizovaná abnormálnou proliferáciou nezrelých buniek hladkého svalu. Progresívne ochorenie postihuje najmä pľúcny parenchým a môže mať až fatálny koniec. Mimopľúcna forma LAM sa najčastejšie prejavuje bolesťou brucha, nádormi v retroperitoneu a chylóznym ascitom. V kazuistikách opisujeme výskyt dvoch prípadov mimopľúcnej formy LAM. V obidvoch prípadoch išlo o pelvickú lokalizáciu nádorov v oblasti fossa obturatoria a vasa iliaca externa. Pacientky boli po operácii primárne liečené gestagénmi. Sirolimus bol liekom druhej línie.
Lymphangioleiomyomatosis (LAM) is a rare progressive disease affecting women of childbearing age. The disease is characterised by an abnormal proliferation of immature smooth muscle cells predominantly in the lung. It gradually leads to respiratory failure, and it frequently result in death. Extrapulmonary LAM typically presents with abdominal mass, abdominal pain and chylous ascites. In the case reports we describe two cases of premenopausal females with extrapulmonary LAM. In both cases they occur in pelvic location in the obturator fossa and around the external iliac artery. After surgical procedures patients were primary treated with progesterone. Sirolimus was second-line drugs.
- Klíčová slova
- Rapamun, Plaquenil, Megace,
- MeSH
- chylózní ascites diagnóza farmakoterapie terapie MeSH
- diferenciální diagnóza MeSH
- dospělí MeSH
- drenáž MeSH
- lidé MeSH
- lymfangioleiomyomatóza * diagnóza terapie MeSH
- lymfatické uzliny chirurgie imunologie mikrobiologie MeSH
- megestrol terapeutické užití MeSH
- nádory dělohy MeSH
- nádory vaječníků MeSH
- retroperitoneální nádory MeSH
- sirolimus terapeutické užití MeSH
- vzácné nemoci diagnóza farmakoterapie terapie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
Lymphogranuloma venereum (LGV) is an infection caused by a subspecies of the bacterium Chlamydia trachomatis (invasive serovars L1, L2, or L3). It primarily causes painful swelling of the lymph nodes located closest to the site of initial infection. If untreated, the disease can progress and patients are needed in order to better consider the statements presented in this article. If untreated, it causes serious skin damages like scarring and genital elephantiasis. LGV is transmitted by direct sexual contact with genitals, rectum, or mouth. Once in the body, Chlamydia Spp. could reproduce themselves in the lymph nodes and could spread to other organs, provoking complications, such as the so-called pelvic inflammatory disease. Both genital and extragenital manifestations of this disease are able to induce serious differential diagnostic difficulties, indirectly leading to progression and dissemination of the infection. The reason for that might be – not very rarely – related to non-typical clinical and histopathological findings. This article presents two unusual cases of LGV with atypical histopathological/clinical findings and also focuses on alternative therapeutical approaches, such as surgical excision at stage 1, leading to positive outcome. However, the question if the surgical method could be used as a standard therapy at stage 1 remains open for discussion. It is not completely clear either if histopathological findings of LGV could reveal progress and/or changes through time as it happens in other diseases like pyoderma gangrenosum. We conclude that both histological and clinical observation on a larger number of patients is needed in order to better consider the statements presented in this article.
- MeSH
- antibakteriální látky terapeutické užití MeSH
- Chlamydia trachomatis izolace a purifikace patogenita MeSH
- chlamydiové infekce diagnóza patofyziologie přenos MeSH
- diferenciální diagnóza MeSH
- dospělí MeSH
- doxycyklin aplikace a dávkování terapeutické užití MeSH
- histologické techniky MeSH
- homosexualita mužská MeSH
- lidé MeSH
- lymfatické uzliny chirurgie mikrobiologie patofyziologie MeSH
- lymphogranuloma venereum diagnóza farmakoterapie mikrobiologie MeSH
- nemoci penisu diagnóza farmakoterapie MeSH
- přenos infekční nemoci MeSH
- senioři nad 80 let MeSH
- sexuálně přenosné nemoci MeSH
- stupeň závažnosti nemoci MeSH
- ústa MeSH
- výsledek terapie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- Publikační typ
- kazuistiky MeSH
- přehledy MeSH
Mycobacterium avium subsp. avium (MAA) and M. a. hominissuis (MAH) belong to the Mycobacterium avium complex (MAC) and are frequently associated with diseases in animals and humans. The aim of this study was to develop a system for rapid and accurate real time quantitative PCR (qPCR) identification and quantification of MAA and MAH. This study included 22 per os infected pigs, of which 10 were infected with MAA, 10 with MAH and 2 were present as a negative control group. From each animal, 21 different tissue samples as well as blood were tested by microscopy, culture and triplex qPCR. The developed triplex qPCR reaction was based on the simultaneous detection of specific insertion sequences, IS901 and IS1245, and also contained an internal amplification control. In both groups of experimentally infected animals, the newly developed triplex qPCR assay proved to be more specific and sensitive in comparison with the other methods used. Contrary to culture examination, triplex qPCR confirmed the infection in all animals infected with MAA, and in eight animals infected with MAH. In conclusion, we developed a quick and sufficiently sensitive triplex qPCR for MAA and MAH detection in tissue and blood samples. From the food safety point of view the presence of MAH in muscles should be considered as a possible threat to human health.
- MeSH
- gastrointestinální trakt mikrobiologie MeSH
- játra mikrobiologie MeSH
- lidé MeSH
- lymfatické uzliny mikrobiologie MeSH
- Mycobacterium avium komplex MeSH
- nemoci prasat mikrobiologie MeSH
- polymerázová řetězová reakce veterinární MeSH
- prasata MeSH
- reprodukovatelnost výsledků MeSH
- senzitivita a specificita MeSH
- tuberkulóza mikrobiologie veterinární MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
From the mandibular lymph nodes of wild red foxes (Vulpes vulpes) hunted in the region of Gmünd, Lower Austria, two gram-negative, oxidase- and urease-positive, coccoid rod-shaped bacteria (strains 257 and 284) were isolated. Cells were fast growing, nonmotile, and agglutinated with monospecific anti-Brucella (M) serum. Both strains were biochemically identified as Ochrobactrum anthropi by using the API 20NE test. However, sequencing of the 16S rRNA and recA genes clearly identified strains 257 and 284 as Brucella spp. Further molecular analysis by omp2a/b gene sequencing, multilocus sequence typing and multilocus variable number tandem repeats analysis revealed Brucella microti, a recently described Brucella species that has originally been isolated from diseased common voles (Microtus arvalis) in South Moravia, Czech Republic in 2000. Our findings demonstrate that B. microti is prevalent in a larger geographic area covering the region of South Moravia and parts of Lower Austria. Foxes could have become infected by ingestion of infected common voles.
- MeSH
- Brucella klasifikace genetika izolace a purifikace MeSH
- brucelóza epidemiologie mikrobiologie veterinární MeSH
- genotyp MeSH
- lišky * MeSH
- lymfatické uzliny mikrobiologie MeSH
- mandibula MeSH
- Ochrobactrum anthropi klasifikace izolace a purifikace MeSH
- proteiny vnější bakteriální membrány genetika MeSH
- RecA-rekombinasy genetika MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenční analýza MeSH
- zeměpis MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Rakousko MeSH
