Here, we provide the first mass molecular screening of medically important mosquitoes for Bartonella species using multiple genetic markers. We examined a total of 72,115 mosquito specimens, morphologically attributed to Aedes vexans (61,050 individuals), Culex pipiens (10,484 individuals) and species of the Anopheles maculipennis complex (581 individuals) for Bartonella spp. The initial screening yielded 63 Bartonella-positive A. vexans mosquitoes (mean prevalence 0.1%), 34 Bartonella-positive C. pipiens mosquitoes (mean prevalence 0.3%) and 158 Bartonella-positive A. maculipennis group mosquitoes (mean prevalence 27.2%). Several different Bartonella ITS sequences were recovered. This study highlights the need for molecular screening of mosquitoes, the most important vectors of arthropod-borne pathogens, for potential bacterial agents.
- MeSH
- bakteriální geny genetika MeSH
- Bartonella klasifikace genetika izolace a purifikace MeSH
- Culicidae klasifikace mikrobiologie MeSH
- DNA bakterií genetika MeSH
- epidemiologické monitorování MeSH
- infekce bakteriemi rodu Bartonella epidemiologie přenos MeSH
- komáří přenašeči klasifikace mikrobiologie MeSH
- mezerníky ribozomální DNA genetika MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Evropa MeSH
In the study, we screened a total of 399 adult sheep keds (Melophagus ovinus) for the presence of RNA and DNA specific for arboviral, bacterial, and protozoan vector-borne pathogens. All investigated keds were negative for flaviviruses, phleboviruses, bunyaviruses, Borrelia burgdorferi, Rickettsia spp., Anaplasma phagocytophilum, "Candidatus Neoehrlichia mikurensis," and Babesia spp. All ked pools were positive for Bartonella DNA. The sequencing of the amplified fragments of the gltA and 16S-23S rRNA demonstrated a 100 % homology with Bartonella melophagi previously isolated from a sheep ked and from human blood in the USA. The identification of B. melophagi in sheep keds in Central Europe highlights needs extending a list of hematophagous arthropods beyond ticks and mosquitoes for a search of emerging arthropod-borne pathogens.
- MeSH
- Anaplasma phagocytophilum genetika izolace a purifikace MeSH
- Anaplasmataceae genetika izolace a purifikace MeSH
- arboviry genetika izolace a purifikace MeSH
- Babesia genetika izolace a purifikace MeSH
- Bartonella genetika izolace a purifikace MeSH
- Diptera mikrobiologie MeSH
- nemoci ovcí epidemiologie mikrobiologie MeSH
- ovce MeSH
- průzkumy a dotazníky MeSH
- Rickettsia genetika izolace a purifikace MeSH
- sekvenční analýza DNA veterinární MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
