SCOPE: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries. METHODS AND RESULTS: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells. CONCLUSION: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.
- MeSH
- antigeny CD95 metabolismus MeSH
- apoptóza účinky léků MeSH
- brusnice s jedlými plody chemie metabolismus MeSH
- buněčné linie MeSH
- chemorezistence MeSH
- DNA metabolismus MeSH
- fosfatidylseriny metabolismus MeSH
- fosforylace účinky léků MeSH
- fragmentace DNA účinky léků MeSH
- kaspasa 3 metabolismus MeSH
- kaspasa 8 metabolismus MeSH
- kaspasa 9 metabolismus MeSH
- lidé MeSH
- mitogenem aktivované proteinkinasy p38 metabolismus MeSH
- nádory tračníku metabolismus patologie MeSH
- ovoce chemie metabolismus MeSH
- proantokyanidiny chemie izolace a purifikace toxicita MeSH
- protein TRAIL toxicita MeSH
- rostlinné extrakty chemie MeSH
- TRAIL receptory metabolismus MeSH
- Vaccinium vitis-idaea chemie metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH