SCOPE: The aim of this work is to identify which proapoptotic pathway is induced in human colon cancer cell lines, in contact with proanthocyanidins extracted from various berries. METHODS AND RESULTS: Proanthocyanidins (Pcys) extracted from 11 berry species are monitored for proapoptotic activities on two related human colon cancer cell lines: SW480-TRAIL-sensitive and SW620-TRAIL-resistant. Apoptosis induction is monitored by cell surface phosphatidylserine (PS) detection. Lowbush blueberry extract triggers the strongest activity. When tested on the human monocytic cell line THP-1, blueberry Pcys are less effective for PS externalisation and DNA fragmentation is absent, highlighting a specificity of apoptosis induction in gut cells. In Pcys-treated gut cell lines, caspase 8 (apoptosis extrinsic pathway) but not caspase 9 (apoptosis intrinsic pathway) is activated after 3 hours through P38 phosphorylation (90 min), emphasizing the potency of lowbush blueberry Pcys to eradicate gut TRAIL-resistant cancer cells. CONCLUSION: We highlight here that berries Pcys, especially lowbush blueberry Pcys, are of putative interest for nutritional chemoprevention of colorectal cancer in view of their apoptosis induction in a human colorectal cancer cell lines.

• MeSH
• antigeny CD95 metabolismus   MeSH
• apoptóza účinky léků   MeSH
• brusnice s jedlými plody chemie   metabolismus   MeSH
• buněčné linie MeSH
• chemorezistence MeSH
• DNA metabolismus   MeSH
• fosfatidylseriny metabolismus   MeSH
• fosforylace účinky léků   MeSH
• fragmentace DNA účinky léků   MeSH
• kaspasa 3 metabolismus   MeSH
• kaspasa 8 metabolismus   MeSH
• kaspasa 9 metabolismus   MeSH
• lidé MeSH
• mitogenem aktivované proteinkinasy p38 metabolismus   MeSH
• nádory tračníku metabolismus   patologie   MeSH
• ovoce chemie   metabolismus   MeSH
• proantokyanidiny chemie   izolace a purifikace   toxicita   MeSH
• protein TRAIL toxicita   MeSH
• rostlinné extrakty chemie   MeSH
• TRAIL receptory metabolismus   MeSH
• Vaccinium vitis-idaea chemie   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• Klíčová slova
• Candilac,
• MeSH
• bakteriální vaginóza farmakoterapie   MeSH
• probiotika MeSH
• Vaccinium vitis-idaea chemie   MeSH