Humoral immunity in mammals relies on the function of two developmentally and functionally distinct B-cell subsets-B1 and B2 cells. While B2 cells are responsible for the adaptive response to environmental antigens, B1 cells regulate the production of polyreactive and low-affinity antibodies for innate humoral immunity. The molecular mechanism of B-cell specification into different subsets is understudied. In this study, we identified lysine methyltransferase NSD2 (MMSET/WHSC1) as a critical regulator of B1 cell development. In contrast to its minor impact on B2 cells, deletion of the catalytic domain of NSD2 in primary B cells impairs the generation of B1 lineage. Thus, NSD2, a histone H3 K36 dimethylase, is the first-in-class epigenetic regulator of a B-cell lineage in mice.
- MeSH
- analýza přežití MeSH
- B-lymfocyty metabolismus MeSH
- histonlysin-N-methyltransferasa chemie metabolismus MeSH
- histony metabolismus MeSH
- humorální imunita MeSH
- katalytická doména * MeSH
- lysin metabolismus MeSH
- metylace MeSH
- myši inbrední C57BL MeSH
- novorozená zvířata MeSH
- přesmyk imunoglobulinových tříd MeSH
- vztahy mezi strukturou a aktivitou MeSH
- zárodečné centrum lymfatické uzliny metabolismus MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, N.I.H., Extramural MeSH