- Publikační typ
- abstrakt z konference MeSH
- MeSH
- adjuvancia imunologická farmakologie MeSH
- buněčné dělení účinky léků MeSH
- cytokiny biosyntéza krev účinky léků MeSH
- finanční podpora výzkumu jako téma MeSH
- lymfocyty mikrobiologie MeSH
- myši MeSH
- růstové látky farmakologie imunologie MeSH
- sperma chemie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- MeSH
- B-buněčný lymfom imunologie patofyziologie MeSH
- cytokiny fyziologie imunologie MeSH
- imunosupresivní léčba veterinární využití MeSH
- proteiny semenné plazmy imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- kongresy MeSH
OBJECTIVE: The effect of the immunosuppressive fraction of boar seminal vesicle fluid (ISF) was tested on the manifestation of adjuvant arthritis (AA) in rats. METHODS: The inhibitory effect of ISF on mitogen-stimulated proliferation of rat lymphocytes was evaluated by immunoassay using bromodeoxyuridine incorporation. Adjuvant arthritis was induced in male Long Evans rats with Mycobacterium butyricum in adjuvant. ISF was administered at the time of the induction of arthritis. At the time of maximal manifestation of the disease, the hind paw swelling and thymus weight were estimated. IgM and IgG in the rat blood sera were quantified by sandwich ELISA. Serum corticosterone was analyzed by radioimmunoassay. Serum NO2-/NO3-were estimated by diazotation. Serum albumin was measured spectrophotometrically. The expression of IL-6 mRNA in peritoneal macrophages was estimated by dot-blot hybridization. RESULTS: Treatment of arthritic rats with ISF attenuated hind paw edema. The production of IgG subclasses dropped in ISF-treated AA rats. The thymus mass and serum albumin concentration were partially restored due to the ISF treatment. Serum corticosterone as well as NO2-/NO3- concentrations were reduced by the ISF effect. The expression of IL-6 in peritoneal macrophages was inhibited in AA rats after ISF treatment. CONCLUSION: ISF attenuated the manifestation of AA in rats and mitigated the inflammation. Immunoglobulin production was most probably inhibited by the decreased proliferation of B lymphocytes.
- MeSH
- artritida experimentální * farmakoterapie imunologie MeSH
- dusičnany krev MeSH
- dusitany krev MeSH
- exprese genu imunologie MeSH
- imunoglobulin G krev MeSH
- imunoglobulin M krev MeSH
- imunosupresiva farmakologie imunologie MeSH
- injekce intraperitoneální MeSH
- interleukin-6 genetika MeSH
- kortikosteron krev MeSH
- krysa rodu rattus MeSH
- messenger RNA analýza MeSH
- peritoneální makrofágy imunologie účinky léků MeSH
- potkani Long-Evans MeSH
- potkani Wistar MeSH
- prasata MeSH
- proteiny semenné plazmy MeSH
- proteiny farmakologie imunologie MeSH
- sérový albumin MeSH
- sperma chemie imunologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- práce podpořená grantem MeSH
