BACKGROUND: Senescence is a fundamental biological process implicated in various pathologies, including cancer. Regarding carcinogenesis, senescence signifies, at least in its initial phases, an anti-tumor response that needs to be circumvented for cancer to progress. Micro-RNAs, a subclass of regulatory, non-coding RNAs, participate in senescence regulation. At the subcellular level micro-RNAs, similar to proteins, have been shown to traffic between organelles influencing cellular behavior. The differential function of micro-RNAs relative to their subcellular localization and their role in senescence biology raises concurrent in situ analysis of coding and non-coding gene products in senescent cells as a necessity. However, technical challenges have rendered in situ co-detection unfeasible until now. METHODS: In the present report we describe a methodology that bypasses these technical limitations achieving for the first time simultaneous detection of both a micro-RNA and a protein in the biological context of cellular senescence, utilizing the new commercially available SenTraGorTM compound. The method was applied in a prototypical human non-malignant epithelial model of oncogene-induced senescence that we generated for the purposes of the study. For the characterization of this novel system, we applied a wide range of cellular and molecular techniques, as well as high-throughput analysis of the transcriptome and micro-RNAs. RESULTS: This experimental setting has three advantages that are presented and discussed: i) it covers a "gap" in the molecular carcinogenesis field, as almost all corresponding in vitro models are fibroblast-based, even though the majority of neoplasms have epithelial origin, ii) it recapitulates the precancerous and cancerous phases of epithelial tumorigenesis within a short time frame under the light of natural selection and iii) it uses as an oncogenic signal, the replication licensing factor CDC6, implicated in both DNA replication and transcription when over-expressed, a characteristic that can be exploited to monitor RNA dynamics. CONCLUSIONS: Consequently, we demonstrate that our model is optimal for studying the molecular basis of epithelial carcinogenesis shedding light on the tumor-initiating events. The latter may reveal novel molecular targets with clinical benefit. Besides, since this method can be incorporated in a wide range of low, medium or high-throughput image-based approaches, we expect it to be broadly applicable.

• MeSH
• epitelové buňky metabolismus   MeSH
• genom MeSH
• jaderné proteiny metabolismus   MeSH
• karcinogeneze MeSH
• kultivované buňky MeSH
• lidé MeSH
• mikro RNA metabolismus   MeSH
• nádory glandulární a epitelové genetika   patologie   ultrastruktura   MeSH
• onkogeny * MeSH
• proteiny buněčného cyklu metabolismus   MeSH
• proteiny metabolismus   MeSH
• stanovení celkové genové exprese MeSH
• stárnutí buněk genetika   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

Cystické nádory vycházející z ledvinného parenchymu jsou heterogenní skupinou jednotek s rozličnou histogenezou, biologickým chováním i prognózou. Ačkoliv agresivní multicystické tumory jsou extrémně vzácné, existují a urologové o nich musí vědět. Mezi typické zástupce multicystických tumorů je řazen multilokulární multicystický renální karcinom, smíšený epiteliální a stromální tumor ledviny a cystický nefrom. Jako cystické tumory lze uvést papilární renální karcinom a světlobuněčný karcinom, avšak tyto tumory jsou cysticky změněné sekundárně. Kavernózní hemangiom či lymfangiom jsou označovány jako multicystické.

Multicystic tumors originated from the kidney parenchyma form heterogenous group of tumors with different histogenesis, biological behavior and prognosis. However aggressive multicystic neoplasias are exceedingly rare, they occur and urologists should recognize that such a tumor could exist. Multilocular cystic clear cell renal cell carcinoma, mixed epithelial and stromal tumor of the kidney and cystic nephroma are considered to be typical examples of this group. Papillary renal cell carcinoma and clear renal cell carcinoma could be also considered as cystic tumors, but cystic change is always secondary. Also cavernous hemangioma and lymphangioma could be assigned as cystic or multicystic tumors.

• MeSH
• cystická onemocnění ledvin patologie   MeSH
• diferenciální diagnóza MeSH
• dospělí MeSH
• lidé MeSH
• nádory cystické, mucinózní a serózní patologie   ultrastruktura   MeSH
• nádory glandulární a epitelové patologie   ultrastruktura   MeSH
• nádory ledvin patologie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• Publikační typ
• přehledy MeSH