The antigenicity, structural location, and function of the predicted lipoprotein TP0136 of Treponema pallidum subsp. pallidum were investigated based on previous screening studies indicating that anti-TP0136 antibodies are present in the sera of syphilis patients and experimentally infected rabbits. Recombinant TP0136 (rTP0136) protein was purified and shown to be strongly antigenic during human and experimental rabbit infection. The TP0136 protein was exposed on the surface of the bacterial outer membrane and bound to the host extracellular matrix glycoproteins fibronectin and laminin. In addition, the TP0136 open reading frame was shown to be highly polymorphic among T. pallidum subspecies and strains at the nucleotide and amino acid levels. Finally, the ability of rTP0136 protein to act as a protective antigen to subsequent challenge with infectious T. pallidum in the rabbit model of infection was assessed. Immunization with rTP0136 delayed ulceration but did not prevent infection or the formation of lesions. These results demonstrate that TP0136 is expressed on the outer membrane of the treponeme during infection and may be involved in attachment to host extracellular matrix components.

• MeSH
• bakteriální vakcíny MeSH
• DNA bakterií genetika   chemie   MeSH
• fibronektiny * metabolismus   MeSH
• fluorescenční mikroskopie MeSH
• imunoelektronová mikroskopie MeSH
• králíci MeSH
• laminin * metabolismus   MeSH
• lidé MeSH
• molekulární sekvence - údaje MeSH
• polymorfismus genetický MeSH
• proteiny vnější bakteriální membrány genetika   imunologie   izolace a purifikace   metabolismus   MeSH
• protilátky bakteriální genetika   imunologie   izolace a purifikace   krev   metabolismus   MeSH
• rekombinantní proteiny imunologie   MeSH
• Treponema pallidum * MeSH
• vazba proteinů MeSH
• zvířata MeSH
• Check Tag
• králíci MeSH
• lidé MeSH
• zvířata MeSH
• Publikační typ
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH

Single-chain antibodies (scFv) exhibiting specific binding to Lawsonia intracellularis were isolated from a phagemid library expressing scFvs molecules on the surface of filamentous bacteriophages. For scFv selection whole bacterial cells were used and individual clones were tested in ELISA test. The total of seven unique clones with different fingerprint profiles was isolated. All clones were able to bind specifically in immunofluorescence assay. This is the first report of species specific recombinant antibodies against L. intracellularis.

• MeSH
• financování organizované MeSH
• Lawsonia (bakterie) imunologie   MeSH
• lidé MeSH
• peptidová knihovna MeSH
• protilátky bakteriální genetika   imunologie   izolace a purifikace   MeSH
• specificita protilátek MeSH
• variabilní oblast imunoglobulinu genetika   imunologie   izolace a purifikace   MeSH
• Check Tag
• lidé MeSH