Porcine reproductive and respiratory syndrome virus (PRRSV) can predispose pigs to secondary respiratory infection with bacteria such as Haemophilus parasuis. Animals infected with both pathogens develop more severe clinical disease. The immune response of porcine alveolar macrophages (PAMs) to simultaneous infection with PRRSV and H. parasuis was analysed in vitro, describing cytokine production, expression of cell surface molecules, and production of reactive oxygen species (ROS). Concurrent infection with PRRSV and H. parasuis increased gene expression of pro-inflammatory cytokines (TNF-α, IL-1β, IL-8) in PAMs in comparison with PAMs infected with PRRSV or H. parasuis alone. An additive effect of dual infection on IL-1β production was confirmed at the protein level. PAMs infected with PRRSV showed increased production of ROS compared to controls. Conversely, simultaneous infection of PAMs with PRRSV and H. parasuis decreased production of ROS, indicating the presence of an H. parasuis defence mechanism against respiratory burst. Concurrent infection of PAMs with PRRSV and H. parasuis was shown to elicit a pro-inflammatory immune response represented by significant IL-1β production. Severe multifactorial respiratory disease in natural conditions caused by both pathogens could be the consequence of pro-inflammatory mediated immunopathology.

• MeSH
• alveolární makrofágy imunologie   mikrobiologie   virologie   MeSH
• biologické markery analýza   MeSH
• cytokiny genetika   imunologie   MeSH
• Haemophilus parasuis imunologie   MeSH
• hemofilové infekce komplikace   imunologie   veterinární   virologie   MeSH
• koinfekce veterinární   MeSH
• kultivované buňky MeSH
• messenger RNA metabolismus   MeSH
• nemoci prasat imunologie   mikrobiologie   virologie   MeSH
• prasata MeSH
• regulace exprese virových genů MeSH
• regulace genové exprese u bakterií MeSH
• reprodukční a respirační syndrom prasat imunologie   virologie   MeSH
• virus reprodukčního a respiračního syndromu prasat imunologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH