The choice of tools that enable efficient targeting of exogenous antigens (Ag) for processing and presentation by professional Ag-presenting cells (APC) remains limited. This represents, indeed, a bottleneck in development of vaccines inducing specific T-cell responses. Here, we describe a novel strategy of Ag delivery into APCs. The Ag of choice is fused to the N- or C-terminus of streptavidin (SA) and tetrameric Ag-SA or SA-Ag fusion proteins are produced in E. coli and purified by 2-Iminobiotin-Agarose affinity chromatography. Alternatively, Ag-SA proteins are purified from urea extracts of E. coli inclusion bodies and refolded in vitro into functional tetramers. Complexes with biotinylated antibodies targeting cell surface receptors are formed and used to deliver the Ags of choice for processing and presentation by APCs and induction of Ag-specific CD4+ and CD8+ T-cell responses in vitro and in vivo.
- MeSH
- antigeny CD11c imunologie metabolismus MeSH
- antigeny chemie genetika imunologie metabolismus MeSH
- biotinylace MeSH
- buněčné linie MeSH
- dendritické buňky imunologie metabolismus MeSH
- kur domácí MeSH
- molekulární sekvence - údaje MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- ovalbumin chemie genetika imunologie metabolismus MeSH
- receptory buněčného povrchu imunologie metabolismus MeSH
- rekombinantní fúzní proteiny chemie genetika imunologie metabolismus MeSH
- rozpustnost MeSH
- sekvence aminokyselin MeSH
- sekvence nukleotidů MeSH
- streptavidin chemie genetika imunologie metabolismus MeSH
- T-lymfocyty imunologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
