Stability studies represent an essential component of pharmaceutical development, enabling critical evaluation of the therapeutic potential of an active pharmaceutical ingredient (API) or a final pharmaceutical product under the influence of various environmental factors. The aim of the present study was to investigate the chemical stability of cannabidiol (CBD) in the form of a solid powder (hereinafter referred to as CBD powder) and also dissolved in sunflower oil. We performed stress studies in accordance with the International Conference on Harmonization (ICH) guidelines, where 5 mg of marketed CBD in the form of a solid powder and in form of oil solution were exposed for 7 and 14, 30, 60, 90, 180, 270, and 365 days to precisely defined temperature and humidity conditions, 25 °C ± 2 °C/60% RH ± 5% and 40 °C ± 2 °C/75% RH ± 5% in both open and closed vials in the dark. CBD powder was significantly more stable than CBD in oil solution. Such finding is important because CBD is often administered dissolved in oil matrix in practice due to very good bioavailability. Thus, the knowledge on admissible shelf time is of paramount importance.
- Klíčová slova
- cannabidiol, cannabinol, degradation, oil matrix, stability study, tetrahydrocannabinol,
- Publikační typ
- časopisecké články MeSH
AIM: To demonstrate and discuss the pros and cons of various conventional and innovative analytical approaches. Methodology & results: Matrix-assisted laser desorption/ionization mass spectrometry imaging (MSI) of tissue sections as well as the extraction of tissue homogenates, blood plasma and dried blood spots coupled with LC-MS were employed to monitor the pharmacokinetics of metformin in mice. The time profile of metformin measured by matrix-assisted laser desorption/ionization MSI correlated well with the results found by LC-MS. Repeatability of the preparation of tissue sections for MSI was very good. CONCLUSION: MSI provided valuable information on the spatial distribution and relative concentration of the analyte within tissue sections. The analysis of the extracts of tissue homogenates, blood plasma and blood spots provided quantitative data on metformin. The dried blood spot approach is a progressive method of sampling, especially in studies where the amount of available blood is limited.
- Klíčová slova
- dried blood spots, mass spectrometry imaging, metformin,
- MeSH
- chromatografie kapalinová metody MeSH
- lidé MeSH
- metformin farmakokinetika MeSH
- myši MeSH
- spektrometrie hmotnostní - ionizace laserem za účasti matrice metody MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- metformin MeSH
AIM: Novel compounds for obesity treatment are currently being studied employing lipidized analogs of anorexigenic neuropeptides. Various analogs of prolactin-releasing peptide have demonstrated their ability to decrease food intake. Adequate analytical tools are required to support corresponding research. Methodology & results: An analytical method was developed that includes simple dilution of plasma samples prior to liquid chromatography-mass spectrometry and employs a monolithic column for the determination of lipidized analogs of prolactin-releasing peptide in complex biological samples. A multiple reaction monitoring approach was applied that included matrix calibration and an internal standard and produced a linear calibration range 20-200 ng ml-1 in rat and macaque plasma samples. CONCLUSION: A straightforward, simple and reliable analytical method was developed satisfying major validation criteria.
- Klíčová slova
- LC–MS, lipopeptides, monolithic column, prolactin-releasing peptide, stability,
- MeSH
- biochemická analýza krve metody MeSH
- chromatografie kapalinová metody MeSH
- hormon uvolňující prolaktin krev chemie MeSH
- kalibrace MeSH
- krysa rodu Rattus MeSH
- lipidy chemie MeSH
- metody pro přípravu analytických vzorků * MeSH
- sekvence aminokyselin MeSH
- tandemová hmotnostní spektrometrie metody MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- hormon uvolňující prolaktin MeSH
- lipidy MeSH
Jana Zemenová talks to Sankeetha Nadarajah, Commissioning Editor: Jana is a PhD student of analytical chemistry in her final year at the University of Chemistry and Technology, Prague. She also works at the Institute of Organic Chemistry and Biochemistry of Czech Academy of Sciences, in the group led by Lenka Maletínská. She has obtained her MSc in Analysis of Drugs and continues working at University of Chemistry and Technology Prague under the supervision of David Sýkora.
- Klíčová slova
- LC–MS, lipopeptides, review, young scientist,
- MeSH
- hmotnostní spektrometrie MeSH
- lipopeptidy analýza farmakokinetika MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- rozhovory MeSH
- Názvy látek
- lipopeptidy MeSH
