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Department of Anatomy Poznan Universi... 1 Department of Cancer Immunology Chair... 1 Department of Diagnostics and Clinica... 1 Department of Histology and Embryolog... 1 Department of Human Morphology and Em... 1 Department of Human Morphology and Em... 1 Department of Obstetrics and Gynaecol... 1 Department of Veterinary Surgery Inst... 1 Division of Anatomy and Histology Uni... 1 Gene Therapy Laboratory Department of... 1 Physiology Graduate Faculty North Car... 1 Prestage Department of Poultry Scienc... 1 Section of Regenerative Medicine and ... 1
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- Autor
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Pracoviště
Department of Anatomy Poznan Universi... 1 Department of Cancer Immunology Chair... 1 Department of Diagnostics and Clinica... 1 Department of Histology and Embryolog... 1 Department of Human Morphology and Em... 1 Department of Human Morphology and Em... 1 Department of Obstetrics and Gynaecol... 1 Department of Veterinary Surgery Inst... 1 Division of Anatomy and Histology Uni... 1 Gene Therapy Laboratory Department of... 1 Physiology Graduate Faculty North Car... 1 Prestage Department of Poultry Scienc... 1 Section of Regenerative Medicine and ... 1
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- Bryl, Rut
- Nawrocki, Mariusz J
- Jopek, Karol
- Kaczmarek, Mariusz
- Bukowska, Dorota
- Antosik, Paweł
- Mozdziak, Paul
- Zabel, Maciej
- Dzięgiel, Piotr
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Kempisty, Bartosz
Autor Kempisty, Bartosz Department of Veterinary Surgery, Institute of Veterinary Medicine, Nicolaus Copernicus University in Torun, 87-100 Torun, Poland Physiology Graduate Faculty, North Carolina State University, Raleigh, NC 27695, USA Department of Human Morphology and Embryology, Division of Anatomy, Wroclaw Medical University, 50-367 Wroclaw, Poland Department of Obstetrics and Gynaecology, University Hospital and Masaryk University, 62500 Brno, Czech Republic
PubMed
37372403
PubMed Central
PMC10297922
DOI
10.3390/genes14061223
PII: genes14061223
Knihovny.cz E-zdroje
Heart failure remains a major cause of death worldwide. There is a need to establish new management options as current treatment is frequently suboptimal. Clinical approaches based on autologous stem cell transplant is potentially a good alternative. The heart was long considered an organ unable to regenerate and renew. However, several reports imply that it may possess modest intrinsic regenerative potential. To allow for detailed characterization of cell cultures, whole transcriptome profiling was performed after 0, 7, 15, and 30 days of in vitro cell cultures (IVC) from the right atrial appendage and right atrial wall utilizing microarray technology. In total, 4239 differentially expressed genes (DEGs) with ratio > abs |2| and adjusted p-value ≤ 0.05 for the right atrial wall and 4662 DEGs for the right atrial appendage were identified. It was shown that a subset of DEGs, which have demonstrated some regulation of expression levels with the duration of the cell culture, were enriched in the following GO BP (Gene Ontology Biological Process) terms: "stem cell population maintenance" and "stem cell proliferation". The results were validated by RT-qPCR. The establishment and detailed characterization of in vitro culture of myocardial cells may be important for future applications of these cells in heart regeneration processes.
- Klíčová slova
- cardiomyocytes, long-term in vitro cell culture, porcine cardiac muscle, stemness markers, transcriptomic analysis,
- MeSH
- buněčné kultury MeSH
- kardiomyocyty * metabolismus MeSH
- prasata MeSH
- regulace genové exprese * MeSH
- stanovení celkové genové exprese MeSH
- transkriptom * MeSH
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- Publikační typ
- časopisecké články MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
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