Metals are widely utilized as implant materials for bone fixtures as well as stents. Biodegradable versions of these implants are highly desirable since patients do not have to undergo a second surgery for the materials to be removed. Attractive options for such materials are zinc silver alloys since they also offer the benefit of being antibacterial. However, it is important to investigate the effect of the degradation products of such alloys on the surrounding cells, taking into account silver cytotoxicity. Here we investigated zinc alloyed with 1 % of silver (Zn1Ag) and how differently concentrated extracts (1 %-100 %) of this material impact human umbilical vein endothelial cells (HUVECs). More specifically, we focused on free radical generation and oxidative stress as well as the impact on cell viability. To determine free radical production we used diamond-based quantum sensing as well as conventional fluorescent assays. The viability was assessed by observing cell morphology and the metabolic activity via the MTT assay. We found that 1 % and 10 % extracts are well tolerated by the cells. However, at higher extract concentrations we observed severe impact on cell viability and oxidative stress. We were also able to show that quantum sensing was able to detect significant free radical generation even at the lowest tested concentrations.

• Klíčová slova
• Biodegradable zinc, Cytotoxicity, Free radical, NV center, Nanodiamond, Relaxometry,
• MeSH
• biokompatibilní materiály chemie   farmakologie   MeSH
• endoteliální buňky pupečníkové žíly (lidské) * účinky léků   MeSH
• lidé MeSH
• nanodiamanty * chemie   MeSH
• oxidační stres * účinky léků   MeSH
• slitiny * chemie   MeSH
• stříbro toxicita   chemie   MeSH
• testování materiálů metody   MeSH
• viabilita buněk * účinky léků   MeSH
• volné radikály metabolismus   MeSH
• vstřebatelné implantáty škodlivé účinky   MeSH
• zinek * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• biokompatibilní materiály MeSH
• nanodiamanty * MeSH
• slitiny * MeSH
• stříbro MeSH
• volné radikály MeSH
• zinek * MeSH