The progress of the oxidative polymerization of aniline with ammonium peroxydisulfate in an aqueous medium has been monitored in situ by attenuated total reflection Fourier transform infrared spectroscopy. The growth of polyaniline film at the crystal surface, as well as the changes proceeding in the surrounding aqueous medium, are reflected in the spectra. The evolution of the spectra during aniline polymerization in the presence of acetic or sulfuric acid was studied with the aim of understanding the influence of acidity on the observed morphology of the final polyaniline films, granular or nanotubes. The changes occurring during polymerization are discussed with the help of differential spectra. Several processes connected with the various stages of aniline oxidation, the evolution of film morphology, or protonation, were distinguished by using factor analysis applied to a large number of spectra obtained in the course of aniline polymerizations on the crystal.
- Publikační typ
- časopisecké články MeSH
Synthesis and preliminary anticancer activity of new star-shaped immunoglobulin-containing polymer-doxorubicin (DOX) conjugates were investigated. The polymer precursors used for the synthesis of immunoglobulin-polymer-drug conjugates are based on N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers, the anticancer drug DOX is attached to the immunoglobulin-modified polymer via a pH-sensitive hydrazone linkage. Such polymer-DOX conjugates are stable in aqueous solution at pH 7.4 (pH of blood plasma) and the drug is released in mildly acid environment at pH 5-5.5 (pH in endosomes or lysosomes of target cells). Semitelechelic copolymer chains are linked to the immunoglobulin via one-point attachment to avoid branching of the conjugate observed in our earlier studied systems. The cytostatic activity of the conjugates tested on several cancer cell lines was similar to that of free DOX.HCl and correlated with the sensitivity of a particular cell line to DOX. The star-shaped conjugates containing immunoglobulin showed a significantly higher antitumor activity in vivo than immunoglobulin-free non-targeted polymer conjugates when tested in mice bearing EL4 T-cell lymphoma.
- MeSH
- antibiotika antitumorózní aplikace a dávkování chemie farmakologie MeSH
- doxorubicin aplikace a dávkování analogy a deriváty chemie farmakologie MeSH
- imunoglobuliny aplikace a dávkování chemie MeSH
- imunokonjugáty aplikace a dávkování chemie MeSH
- kyseliny polymethakrylové aplikace a dávkování chemie farmakologie MeSH
- léky antitumorózní - screeningové testy MeSH
- lidé MeSH
- lymfom T-buněčný farmakoterapie MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- nádorové buněčné linie MeSH
- nosiče léků aplikace a dávkování chemická syntéza chemie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antibiotika antitumorózní MeSH
- doxorubicin-N-(2-hydroxypropyl)methacrylamide copolymer conjugate MeSH Prohlížeč
- doxorubicin MeSH
- imunoglobuliny MeSH
- imunokonjugáty MeSH
- kyseliny polymethakrylové MeSH
- nosiče léků MeSH
A new thermoresponsive system designed for local radiotherapy has been developed. In this system a radionuclide complex is entrapped in a thermoresponsive polymer locally precipitated at body temperature after injection of a polymer-complex solution into the tissue where a therapeutic effect is required. The lifetime of the system is controlled by the rate of polymer hydrolysis, its dissolution and elimination from the body. The thermoresponsive polymer with the cloud temperature (CT) below body temperature is based on copolymers of N-isopropylmethacrylamide with a methacrylamide-type comonomer containing hydrophobic n-alkyls of three different sizes (C(3), C(6) and C(12)) bonded by a hydrolytically labile hydrazone bond. Hydrolysis of hydrazone bond results in a copolymer soluble at body temperature. The copolymer containing 27.5 mole% of the comonomer with the C(6) moiety, which was chosen for further study, has the CT 22 degrees C and its phase separation is complete at 34 degrees C. Polymer dissolution is complete within 48 h at both pH 5.0 or 7.4. The model therapeutic radionuclide, (64)Cu, in the form of its hydrophobic chelate bis(quinolin-8-olato-N,O) [(64)Cu]copper, is efficiently kept hydrophobically entrapped in the phase-separated polymer until the dissolution by hydrolytic degradation is completed.
- MeSH
- akrylamidy chemická syntéza chemie MeSH
- disulfidy chemická syntéza chemie MeSH
- doxorubicin chemická syntéza chemie MeSH
- oligopeptidy chemická syntéza chemie MeSH
- technika přenosu genů * MeSH
- thiazolidiny chemická syntéza chemie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 2-mercaptothiazoline MeSH Prohlížeč
- akrylamidy MeSH
- disulfidy MeSH
- doxorubicin MeSH
- N-(2-hydroxypropyl)methacrylamide co-polymer-doxorubicin conjugate MeSH Prohlížeč
- N-(2-hydroxypropyl)methacrylamide MeSH Prohlížeč
- oligopeptidy MeSH
- thiazolidiny MeSH
