The nucleolar protein 2 gene encodes a protein specific for the nucleolus. It is assumed that it plays a role in the synthesis of ribosomes and regulation of the cell cycle. Due to its link to cell proliferation, higher expression of Nop2 indicates a worse tumor prognosis. In this work we used Nop2(gt1gaj) gene trap mouse strain. While lethality of homozygous animals suggested a vital role of this gene, heterozygous animals allowed the detection of expression of Nop2 in various tissues, including mouse brain. Histochemistry, immunohistochemistry and immunoelectron microscopy techniques, applied to a mature mouse brain, human brain and on mouse neural stem cells revealed expression of Nop2 in differentiating cells, including astrocytes, as well as in mature neurons. Nop2 was detected in various regions of mouse and human brain, mostly in large pyramidal neurons. In the human, Nop2 was strongly expressed in supragranular and infragranular layers of the somatosensory cortex and in layer III of the cingulate cortex. Also, Nop2 was detected in CA1 and the subiculum of the hippocampus. Subcellular analyses revealed predominant location of Nop2 within the dense fibrillar component of the nucleolus. To test if Nop2 expression correlates to cell proliferation occurring during tissue regeneration, we induced strokes in mice by middle cerebral artery occlusion. Two weeks after stroke, the number of Nop2/nestin double positive cells in the region affected by ischemia and the periventricular zone substantially increased. Our findings suggest a newly discovered role of Nop2 in both mature neurons and in cells possibly involved in the regeneration of nervous tissue.

• Klíčová slova
• Brain, Cell cycle, Nop2, Nucleolus, Stem cells, Stroke,
• MeSH
• astrocyty fyziologie   MeSH
• cévní mozková příhoda patofyziologie   MeSH
• dospělí MeSH
• infarkt arteria cerebri media MeSH
• jaderné proteiny genetika   metabolismus   MeSH
• kultivované buňky MeSH
• lidé MeSH
• modely nemocí na zvířatech MeSH
• mozek fyziologie   patofyziologie   MeSH
• myši inbrední C57BL MeSH
• myši transgenní MeSH
• nervové kmenové buňky fyziologie   MeSH
• nestin metabolismus   MeSH
• neurogeneze fyziologie   MeSH
• neurony fyziologie   MeSH
• proliferace buněk fyziologie   MeSH
• proteinmethyltransferasy MeSH
• tRNA-methyltransferasy metabolismus   MeSH
• zvířata MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• jaderné proteiny MeSH
• Nes protein, mouse MeSH Prohlížeč
• nestin MeSH
• NOP2 protein, human MeSH Prohlížeč
• Nop2 protein, mouse MeSH Prohlížeč
• proteinmethyltransferasy MeSH
• tRNA-methyltransferasy MeSH