Nop2 is expressed during proliferation of neural stem cells and in adult mouse and human brain
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
25481415
DOI
10.1016/j.brainres.2014.11.040
PII: S0006-8993(14)01624-2
Knihovny.cz E-zdroje
- Klíčová slova
- Brain, Cell cycle, Nop2, Nucleolus, Stem cells, Stroke,
- MeSH
- astrocyty fyziologie MeSH
- cévní mozková příhoda patofyziologie MeSH
- dospělí MeSH
- infarkt arteria cerebri media MeSH
- jaderné proteiny genetika metabolismus MeSH
- kultivované buňky MeSH
- lidé MeSH
- modely nemocí na zvířatech MeSH
- mozek fyziologie patofyziologie MeSH
- myši inbrední C57BL MeSH
- myši transgenní MeSH
- nervové kmenové buňky fyziologie MeSH
- nestin metabolismus MeSH
- neurogeneze fyziologie MeSH
- neurony fyziologie MeSH
- proliferace buněk fyziologie MeSH
- proteinmethyltransferasy MeSH
- tRNA-methyltransferasy metabolismus MeSH
- zvířata MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- jaderné proteiny MeSH
- Nes protein, mouse MeSH Prohlížeč
- nestin MeSH
- NOP2 protein, human MeSH Prohlížeč
- Nop2 protein, mouse MeSH Prohlížeč
- proteinmethyltransferasy MeSH
- tRNA-methyltransferasy MeSH
The nucleolar protein 2 gene encodes a protein specific for the nucleolus. It is assumed that it plays a role in the synthesis of ribosomes and regulation of the cell cycle. Due to its link to cell proliferation, higher expression of Nop2 indicates a worse tumor prognosis. In this work we used Nop2(gt1gaj) gene trap mouse strain. While lethality of homozygous animals suggested a vital role of this gene, heterozygous animals allowed the detection of expression of Nop2 in various tissues, including mouse brain. Histochemistry, immunohistochemistry and immunoelectron microscopy techniques, applied to a mature mouse brain, human brain and on mouse neural stem cells revealed expression of Nop2 in differentiating cells, including astrocytes, as well as in mature neurons. Nop2 was detected in various regions of mouse and human brain, mostly in large pyramidal neurons. In the human, Nop2 was strongly expressed in supragranular and infragranular layers of the somatosensory cortex and in layer III of the cingulate cortex. Also, Nop2 was detected in CA1 and the subiculum of the hippocampus. Subcellular analyses revealed predominant location of Nop2 within the dense fibrillar component of the nucleolus. To test if Nop2 expression correlates to cell proliferation occurring during tissue regeneration, we induced strokes in mice by middle cerebral artery occlusion. Two weeks after stroke, the number of Nop2/nestin double positive cells in the region affected by ischemia and the periventricular zone substantially increased. Our findings suggest a newly discovered role of Nop2 in both mature neurons and in cells possibly involved in the regeneration of nervous tissue.
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