Nop2 is expressed during proliferation of neural stem cells and in adult mouse and human brain
Language English Country Netherlands Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
25481415
DOI
10.1016/j.brainres.2014.11.040
PII: S0006-8993(14)01624-2
Knihovny.cz E-resources
- Keywords
- Brain, Cell cycle, Nop2, Nucleolus, Stem cells, Stroke,
- MeSH
- Astrocytes physiology MeSH
- Stroke physiopathology MeSH
- Adult MeSH
- Infarction, Middle Cerebral Artery MeSH
- Nuclear Proteins genetics metabolism MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Disease Models, Animal MeSH
- Brain physiology physiopathology MeSH
- Mice, Inbred C57BL MeSH
- Mice, Transgenic MeSH
- Neural Stem Cells physiology MeSH
- Nestin metabolism MeSH
- Neurogenesis physiology MeSH
- Neurons physiology MeSH
- Cell Proliferation physiology MeSH
- Protein Methyltransferases MeSH
- tRNA Methyltransferases metabolism MeSH
- Animals MeSH
- Check Tag
- Adult MeSH
- Humans MeSH
- Male MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Nuclear Proteins MeSH
- Nes protein, mouse MeSH Browser
- Nestin MeSH
- NOP2 protein, human MeSH Browser
- Nop2 protein, mouse MeSH Browser
- Protein Methyltransferases MeSH
- tRNA Methyltransferases MeSH
The nucleolar protein 2 gene encodes a protein specific for the nucleolus. It is assumed that it plays a role in the synthesis of ribosomes and regulation of the cell cycle. Due to its link to cell proliferation, higher expression of Nop2 indicates a worse tumor prognosis. In this work we used Nop2(gt1gaj) gene trap mouse strain. While lethality of homozygous animals suggested a vital role of this gene, heterozygous animals allowed the detection of expression of Nop2 in various tissues, including mouse brain. Histochemistry, immunohistochemistry and immunoelectron microscopy techniques, applied to a mature mouse brain, human brain and on mouse neural stem cells revealed expression of Nop2 in differentiating cells, including astrocytes, as well as in mature neurons. Nop2 was detected in various regions of mouse and human brain, mostly in large pyramidal neurons. In the human, Nop2 was strongly expressed in supragranular and infragranular layers of the somatosensory cortex and in layer III of the cingulate cortex. Also, Nop2 was detected in CA1 and the subiculum of the hippocampus. Subcellular analyses revealed predominant location of Nop2 within the dense fibrillar component of the nucleolus. To test if Nop2 expression correlates to cell proliferation occurring during tissue regeneration, we induced strokes in mice by middle cerebral artery occlusion. Two weeks after stroke, the number of Nop2/nestin double positive cells in the region affected by ischemia and the periventricular zone substantially increased. Our findings suggest a newly discovered role of Nop2 in both mature neurons and in cells possibly involved in the regeneration of nervous tissue.
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