A series of lichen secondary metabolites (parietin, atranorin, usnic and gyrophoric acid) and their interactions with calf thymus DNA were investigated using molecular biophysics and biochemical methods. The binding constants K were estimated to range from 4.3×10(5) to 2.4×10(7)M(-1) and the percentage of hypochromism was found to be 16-34% (from spectral titration). The results of spectral measurement indicate that the compounds act as effective DNA-interacting agents. Electrophoretic separation studies prove that from all the metabolites tested in this study, only gyrophoric acid exhibited an inhibitory effect on Topo I (25μM).

• Klíčová slova
• DNA-binding, Lichens, Topoisomerase I, II,
• MeSH
• benzoáty farmakologie   MeSH
• benzofurany farmakologie   MeSH
• cirkulární dichroismus MeSH
• DNA účinky léků   metabolismus   MeSH
• emodin analogy a deriváty   farmakologie   MeSH
• hydroxybenzoáty farmakologie   MeSH
• inhibitory topoisomerasy I farmakologie   MeSH
• inhibitory topoisomerasy II farmakologie   MeSH
• interkalátory farmakologie   MeSH
• kinetika MeSH
• konformace nukleové kyseliny MeSH
• lidé MeSH
• lišejníky chemie   metabolismus   MeSH
• skot MeSH
• spektrofotometrie ultrafialová MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• skot MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• atranorin MeSH Prohlížeč
• benzoáty MeSH
• benzofurany MeSH
• calf thymus DNA MeSH Prohlížeč
• DNA MeSH
• emodin MeSH
• gyrophoric acid MeSH Prohlížeč
• hydroxybenzoáty MeSH
• inhibitory topoisomerasy I MeSH
• inhibitory topoisomerasy II MeSH
• interkalátory MeSH
• physcione MeSH Prohlížeč
• usnic acid MeSH Prohlížeč