Plum pox virus (PPV, family Potyviridae) is one of the most important viral pathogens of Prunus spp. causing considerable damage to stone-fruit industry worldwide. Among the PPV strains identified so far, only PPV-C, PPV-CR, and PPV-CV are able to infect cherries under natural conditions. Herein, we evaluated the pathogenic potential of two viral isolates in herbaceous host Nicotiana benthamiana. Significantly higher accumulation of PPV capsid protein in tobacco leaves infected with PPV-CR (RU-30sc isolate) was detected in contrast to PPV-C (BY-101 isolate). This result correlated well with the symptoms observed in the infected plants. To further explore the host response upon viral infection at the molecular level, a comprehensive proteomic profiling was performed. Using reverse-phase ultra-high-performance liquid chromatography followed by label-free mass spectrometry quantification, we identified 38 unique plant proteins as significantly altered due to the infection. Notably, the abundances of photosynthesis-related proteins, mainly from the Calvin-Benson cycle, were found more aggressively affected in plants infected with PPV-CR isolate than those of PPV-C. This observation was accompanied by a significant reduction in the amount of photosynthetic pigments extracted from the leaves of PPV-CR infected plants. Shifts in the abundance of proteins that are involved in stimulation of photosynthetic capacity, modification of amino acid, and carbohydrate metabolism may affect plant growth and initiate energy formation via gluconeogenesis in PPV infected N. benthamiana. Furthermore, we suggest that the higher accumulation of H2O2 in PPV-CR infected leaves plays a crucial role in plant defense and development by activating the glutathione synthesis.

• Klíčová slova
• LC−MS, antioxidants, compatible interaction, label-free quantification, photosynthesis, sharka, tobacco,
• MeSH
• chlorofyl biosyntéza   MeSH
• chromatografie s reverzní fází MeSH
• energetický metabolismus genetika   MeSH
• fotosyntéza genetika   MeSH
• genotyp MeSH
• glutathion biosyntéza   MeSH
• hmotnostní spektrometrie MeSH
• interakce hostitele a patogenu genetika   MeSH
• karotenoidy biosyntéza   MeSH
• listy rostlin genetika   metabolismus   virologie   MeSH
• nemoci rostlin genetika   virologie   MeSH
• oxidace-redukce MeSH
• peroxid vodíku metabolismus   MeSH
• proteiny tepelného šoku klasifikace   genetika   metabolismus   MeSH
• Prunus avium virologie   MeSH
• regulace genové exprese u rostlin * MeSH
• rostlinné proteiny klasifikace   genetika   metabolismus   MeSH
• slivoň švestka virologie   MeSH
• tabák genetika   metabolismus   virologie   MeSH
• virus šarky švestky klasifikace   genetika   růst a vývoj   patogenita   MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• chlorofyl MeSH
• glutathion MeSH
• karotenoidy MeSH
• peroxid vodíku MeSH
• proteiny tepelného šoku MeSH
• rostlinné proteiny MeSH