Acid-base disorders were studied in two groups of dairy cows. According to conventional interpretation in the 1st group of dairy cows (n = 10) the results of the acid-base parameters indicated respiratory alkalosis and in the 2nd group (n = 10) metabolic acidosis. The data of the two groups were examined using principal component analysis. So called "reduced variables"--principal components for each animal were calculated. Each experimental animal was projected in the coordinates of 3 principal components K1, K2 and K3. The components K1, K2 and K3 comprised a 90% data variability. The importance of variables for a dividing of groups (1 and 2) was quantified. The most important were pCO2, (HCO3)' and BE. It was proved according to mathematico-empiric equations that dairy cows of 1st group suffered from acute respiratory alkalosis. In the 2nd group of dairy cows the combined acid-base disorder was confirmed using the equations for metabolic acidosis and chronic respiratory acidosis. The use of PCA method and empiric equations for acid-base imbalances shows the possibilities for gaining new knowledge how to make the diagnostic process more exact.
- MeSH
- biologické modely MeSH
- mlékárenství MeSH
- nemoci skotu metabolismus MeSH
- poruchy acidobazické rovnováhy metabolismus veterinární MeSH
- skot MeSH
- statistika jako téma MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
The internal environment of 17 dairy cows was observed by help of 23 variables of metabolic profile in blood and milk at lowered titratable acidity of milk (Tab. I) Average values of the observed variables were compared with reference values in a histogramme (Fig. 1). We calculated the statistical significance of differences of arithmetical means from reference average. Using correlation analysis we calculated the correlation coefficients for the observed variables. We represented the chosen correlation relations in correlogrammes (Fig. 2). We found significant correlation relations between AST and titratable acidity (degrees SH) (r = 0.791), AST and milk pH (r 0.617), AST and lactose (r = 0.69), AST and glucose (r = -0.56), blood pH and milk pH (r = -0.608), milk pH and lactose (r = -0.89). Mutual relations of titratable acidity with chosen variables of blood serum and milk were evaluated by help of regression coefficients, calculated for each parameter from the mathematical model (Fig. 3). The nearest relations were found for blood pH (regression coefficient /k/ kPH = -11.9), for AST (kAST = -3.1), for milk pH (k milk pH = -1.72) and for Mg (k Mg = -1.98). We also compared the recorded results of titratable acidity with theoretical values of titratable acidity calculated from the mathematical model (Tab. II). The differences between them were not statistically significant. It is evident from the results that the acid-base balance and functional liver condition influence the titratable acidity of milk.
- MeSH
- koncentrace vodíkových iontů MeSH
- mléko chemie MeSH
- nemoci jater veterinární MeSH
- nemoci skotu * diagnóza MeSH
- skot MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
The internal environment of high-yielding dairy cows was investigated in relation to 36 variables of the blood metabolic profile, blood serum, milk and urine, with the incidence of reduced titration acidity milk syndrome. The levels of variables were confronted with references values. At the same time the statistical significance of differences of arithmetical means from the reference mean was calculated. Correlation analysis of all variables yielded 630 correlation coefficients. Certain correlations were represented in form of correlogrammes. It has been demonstrated that the reduced titration acidity milk syndrome with the lowest values of 1.9 ml 0.25N NaOH per 100 ml occurred after administration of the feed ration deficient both in digestible crude protein and starch units (nutritive ratio 1:7.5) which contained unsafe grass haylage with a high content of ammonia (232.9 mg per 100 g). A highly significant negative correlation was proved to exist between lactose concentration and pH value of milk (r = -0.913); the regression equation (lactose) is 36.997--4.7536 (pH).
- MeSH
- koncentrace vodíkových iontů MeSH
- mléko analýza MeSH
- skot metabolismus MeSH
- zvířata MeSH
- Check Tag
- skot metabolismus MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
Fifty dairy cows with metabolic alkalosis and forty-eight dairy cows of a control group were subjected to a metabolic profile test with 30 parameters. The results of analyses were confronted with reference values in histograms. We calculated mutual correlation coefficients for all parameters of dairy cows of the two groups. The correlations between selected pairs of parameters were represented diagrammatically in a form of correlograms. We demonstrated that the values of certain parameters being approximately identical, the correlations in group A of dairy cows (with alkalosis) varied in a different way from the correlations of the control group. It is seen from the results that the confrontation of the values of a parameter with reference values need not be in all cases a reliable criterion of evaluating the internal environment in dairy cows. We processed the metabolic profiles of the two groups by a multidimensional analysis of principal components. The dairy cows of the two groups clearly differentiated from one another according to the internal environment regarding the used parameters. We draw attention to a possibility of computerizing the diagnostic significance of the metabolic profile parameters.
Blood and urine samples of 180 dairy cows from 12 herds were examined. Sodium and potassium concentrations were determined in erythrocytes, whole blood, plasma, urine, and glucose concentration in blood: the interrelations were compared. Sodium and potassium concentrations in erythrocytes were 85.15 +/- 11.45 mmol/l, and 25.93 +/- 7.81 mmol/l, respectively. A statistically significant relation was found between sodium and potassium concentrations in erythrocytes (r = 0.3467+++) and the content of electrolyte in blood cells and in whole blood (Na: r = 0.5336+++; K: r = 0.3561+++). No statistically significant relation of intraerythrocyte concentration of both electrolytes was confirmed with respect to the other characteristics (sodium and potassium concentrations in plasma and urine, and glucose concentration in blood). In the conditions of routine laboratory diagnostics in clinically healthy cows, determinations of intraerythrocyte sodium and potassium do not broaden possibilities of evaluating the metabolic state of these electrolytes and cannot be used as the characteristics of energy insufficiency. The cannot replace the analyses performed in serum and urine.
- MeSH
- draslík krev moč MeSH
- erytrocyty analýza MeSH
- krevní glukóza analýza MeSH
- krevní plazma analýza MeSH
- mlékárenství MeSH
- skot krev MeSH
- sodík krev moč MeSH
- zvířata MeSH
- Check Tag
- skot krev MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- draslík MeSH
- krevní glukóza MeSH
- sodík MeSH
The influence of citronellylseneciate on the digestibility of nutrients and energy, as well as on the level of some rumen and blood metabolites, was studied in feed rations for rams. Citronellylseneciate was not found to have a significant influence on the digestibility of nutrients and energy and nitrogen balance in feed ration. Neither did citronellylseneciate influence the production of total volatile fatty acids and the molar percent of acetic, propionic and n-butyric acids. The addition of citronellylseneciate to feed ration resulted in a decrease in the pH value in rumen and in a non-significant increase in ammonia in rumen and urea in blood. The hematological profile showed increased levels of phosphorus and glutamate-oxalate transferase.
- MeSH
- bachor metabolismus MeSH
- butyráty farmakologie MeSH
- krmivo pro zvířata MeSH
- krotonáty farmakologie MeSH
- monoterpeny * MeSH
- ovce metabolismus fyziologie MeSH
- přijímání potravy účinky léků MeSH
- terpeny farmakologie MeSH
- trávení účinky léků MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- butyráty MeSH
- citronellyl seneciate MeSH Prohlížeč
- krotonáty MeSH
- monoterpeny * MeSH
- terpeny MeSH
Gestation age of a neonate could be estimated approximately by a pathologist according to some macroscopical features. In addition to the weight of large organs the authors studied weight of other organs and some biometrical data in a group of 84 normotrophic neonates. Regressive analysis gave information about the growth trend of organs; compared with tables it enabled to find out the growth retardation of a neonate or his single organ. The span of fingers represented a precise statistical figure for the estimation of gestation age in addition to the weight of organs.
- MeSH
- antropometrie * MeSH
- gestační stáří MeSH
- lidé MeSH
- novorozenec * MeSH
- porodní hmotnost * MeSH
- velikost orgánu MeSH
- Check Tag
- lidé MeSH
- novorozenec * MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- MeSH
- apolipoproteiny krev MeSH
- dietní tuky aplikace a dávkování MeSH
- dospělí MeSH
- lidé MeSH
- lipidy krev MeSH
- lipoproteiny krev MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- apolipoproteiny MeSH
- dietní tuky MeSH
- lipidy MeSH
- lipoproteiny MeSH
- MeSH
- arterioskleróza krev MeSH
- cholesterol krev MeSH
- HDL-cholesterol MeSH
- LDL-cholesterol MeSH
- lidé MeSH
- lipoproteiny HDL krev MeSH
- lipoproteiny LDL krev MeSH
- lipoproteiny VLDL krev MeSH
- triglyceridy krev MeSH
- vitamin E farmakologie MeSH
- VLDL-cholesterol MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- cholesterol MeSH
- HDL-cholesterol MeSH
- LDL-cholesterol MeSH
- lipoproteiny HDL MeSH
- lipoproteiny LDL MeSH
- lipoproteiny VLDL MeSH
- triglyceridy MeSH
- vitamin E MeSH
- VLDL-cholesterol MeSH
- MeSH
- adheziva škodlivé účinky MeSH
- arterioskleróza chemicky indukované MeSH
- cholesterol krev MeSH
- dospělí MeSH
- fenoly škodlivé účinky MeSH
- formaldehyd škodlivé účinky MeSH
- HDL-cholesterol MeSH
- lidé středního věku MeSH
- lidé MeSH
- lipoproteiny HDL krev MeSH
- nemoci z povolání chemicky indukované MeSH
- polymery * MeSH
- syntetické pryskyřice škodlivé účinky MeSH
- triaziny škodlivé účinky MeSH
- uhlovodíky škodlivé účinky MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- adheziva MeSH
- cholesterol MeSH
- fenoly MeSH
- formaldehyd MeSH
- HDL-cholesterol MeSH
- lipoproteiny HDL MeSH
- melamine bitumens MeSH Prohlížeč
- phenol-formaldehyde resin MeSH Prohlížeč
- polymery * MeSH
- syntetické pryskyřice MeSH
- triaziny MeSH
- uhlovodíky MeSH