Five different hybridoma clones secreting anti-HBeAg antibody were constructed by fusing cells of mouse myeloma line SP2/0 with splenocytes from BALB/c mice immunized with recombinant HBeAg. The monoclonal antibodies obtained were characterized immunologically and one was used to develop ELISA for detection of HBeAg and anti-HBeAg antibody. These monoclonal assays enabled the detection of 3 U HBeAg/ml and 1 U anti-HBeAg/ml with reference to standards of the Paul Ehrlich Institute, Frankfurt, F.R.G. Both assays compared well with a commercially available kit (Abbott Laboratory) and were used for detection of HBeAg and anti-HBeAg antibody in clinical serum samples.
- MeSH
- buněčné linie MeSH
- hepatitida B - antigeny e imunologie MeSH
- hepatitida B - antigeny povrchové imunologie MeSH
- hepatitida B - protilátky analýza MeSH
- hepatitida B diagnóza MeSH
- hybridomy imunologie MeSH
- lidé MeSH
- monoklonální protilátky imunologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- rekombinantní proteiny imunologie MeSH
- virus hepatitidy B imunologie MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- hepatitida B - antigeny e MeSH
- hepatitida B - antigeny povrchové MeSH
- hepatitida B - protilátky MeSH
- monoklonální protilátky MeSH
- rekombinantní proteiny MeSH
Age-dependent decline of the anti-tumour efficacy of local IL-2 immunotherapy and chemoimmunotherapy utilizing human recombinant interleukin 2 and cyclophosphamide was investigated in B10 mice bearing syngeneic MC-induced sarcoma. Repeated peritumoral injections of rIL-2 substantially inhibited growth of transplantable MC-induced sarcomas in syngeneic young adult mice whereas no effect was observed in the aged mice. Chemoimmunotherapy of the MC-induced sarcomas in the young adult and in the aged mice treated with cyclophosphamide plus rIL-2 revealed that subthreshold doses of CY were capable of significantly potentiating the immunotherapeutic effect of rIL-2 in young adult mice but not in the aged mice.
- MeSH
- cyklofosfamid terapeutické užití MeSH
- experimentální sarkom chemicky indukované farmakoterapie MeSH
- imunoterapie MeSH
- interleukin-2 terapeutické užití MeSH
- lidé MeSH
- methylcholanthren MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- rekombinantní proteiny terapeutické užití MeSH
- stárnutí * MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- cyklofosfamid MeSH
- interleukin-2 MeSH
- methylcholanthren MeSH
- rekombinantní proteiny MeSH
In the present study, solid phase enzyme immunoassay utilizing antibodies against synthetic IL-2 peptides was used for quantitative measurements of human recombinant and lymphoid IL-2 preparations. The 27-peptide MCF-III-6 (Leu-Glu-His-Leu-Leu-Leu-Asp-Leu-Gln-Met-Ile-Leu-Asn-Gly-Ile-Asn-Asn-- Tyr-Lys-Asn-Pro-Lys-Leu-Thr-Arg-Met-Leu) that comprises the region 14-40 from the IL-2 amino acid sequence was synthesized and used for immunization of rabbits. Resulting anti-MCF-III-6 polyvalent rabbit antibodies reacted specifically in EIA up to dilution of 10(-7) with the MCF-III-6 peptide used for immunization as well as with 16-peptide I-16 (Cys-Nle-Gly-Ile-Asn-Asn-Tyr-Lys-Asn-Pro-Lys-Leu-Thr-Arg-Met-Leu) that comprises the region 27-40 from the IL-2 amino acid sequence. The anti-MCF-III-6 antibody reacted also with human recombinant IL-2 preparations obtained from three producers (Cetus, Riga and Amersham), to the concentration of 0.1 ng/ml, and with various human lymphoid IL-2 preparations. Direct correlation was observed between quantitative measurements of human lymphoid IL-2 by EIA and by CTLL bioassay. It can be concluded that utilization of synthetic IL-2 peptides provides a suitable and comparatively unexpensive immunogen for the production of IL-2 antibodies and that the solid phase EIA using such antibodies can be employed as a rapid, reproducible, and sensitive method for quantitative examination of both recombinant and lymphoid IL-2 preparations.
- MeSH
- aktivace lymfocytů MeSH
- buněčné linie MeSH
- imunoenzymatické techniky * MeSH
- interleukin-2 analýza imunologie MeSH
- králíci MeSH
- lidé MeSH
- molekulární sekvence - údaje MeSH
- peptidy chemická syntéza imunologie MeSH
- protilátky analýza MeSH
- rekombinantní proteiny imunologie MeSH
- sekvence aminokyselin MeSH
- T-lymfocyty imunologie MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- interleukin-2 MeSH
- peptidy MeSH
- protilátky MeSH
- rekombinantní proteiny MeSH
