Malignant gliomas are the most common type of primary malignant brain tumours, characterized by extreme proliferation and aggressive invasion. There is evidence for over-expression of the YKL40 gene in high-grade gliomas. The high serum levels of the glycoprotein are associated with poor prognosis of various inflammatory and tumour processes. We investigated the YKL40 mRNA level and protein expression in the tumour site and in the serum of high-grade glioma patients. The YKL-40 expression in 36 patients with glial tumours (astrocytoma grade III, glioblastoma) and 33 age-matched healthy persons was measured by gene analysis, immunohistochemistry and ELISA. YKL-40 serum levels in high-grade glioma patients compared to healthy subjects were significantly increased (P ≤ 0.05). A wide range of variability in YKL40 mRNA expression was found. YKL-40 staining in situ was more abundant in glioblastoma tissue than in anaplastic astrocytoma, with the lowest level in normal brain tissue. Our gene analysis revealed that in general, YKL40 mRNA in glioma patients was over-expressed versus normal brain. A significant correlation between YKL40 transcript and protein levels was observed (P ≤ 0.05). It could be speculated that the YKL-40 protein might contribute to glioblastomas' specific biological characteristics that distinguish them from grade III gliomas. A complex investigation of YKL40 expression was performed at the molecular and cellular levels in human high-grade gliomas. Serum YKL-40 concentrations increased with tumour grade and correlated positively with transcript rate, being the highest in glioblastoma. We provide evidence for a relationship between YKL40 expression and the malignancy of glial tumours.
- MeSH
- adipokiny biosyntéza krev genetika MeSH
- astrocytom metabolismus patologie terapie MeSH
- dospělí MeSH
- glioblastom metabolismus patologie terapie MeSH
- gliom metabolismus patologie terapie MeSH
- kombinovaná terapie MeSH
- lektiny biosyntéza krev genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- messenger RNA biosyntéza genetika MeSH
- nádorové proteiny biosyntéza krev genetika MeSH
- nádory mozku metabolismus patologie terapie MeSH
- prognóza MeSH
- protein CHI3L1 MeSH
- regulace genové exprese u nádorů MeSH
- RNA nádorová biosyntéza genetika MeSH
- senioři MeSH
- studie případů a kontrol MeSH
- stupeň nádoru MeSH
- upregulace MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adipokiny MeSH
- CHI3L1 protein, human MeSH Prohlížeč
- lektiny MeSH
- messenger RNA MeSH
- nádorové proteiny MeSH
- protein CHI3L1 MeSH
- RNA nádorová MeSH
