Differential expression of HLA-DQA1 and HLA-DQB1 gene alleles was analysed in three different cell populations isolated from peripheral blood-B lymphocytes, monocytes and whole-blood cells. Interallelic differences in mRNA levels were observed: DQA1*03 alleles were among the most expressed in all cell types, whereas DQA1*05 alleles were least expressed in whole blood and monocytes and among the most expressed in B cells. For DQB1 gene, DQB1*06 group of alleles were the most expressed, and DQB1*02 group the least expressed within all cell populations examined. In comparison with the rest alleles, DQB1*06 and DQB1*05:02 alleles have higher expression in monocytes than in B cells, professional antigen-presenting cells. Cell type-specific regulation of expression was observed as well, with higher and more balanced expression of alleles in B lymphocytes compared to monocytes.
- Klíčová slova
- HLA class II, HLA-DQA1, HLA-DQB1, antigen presentation, gene expression, genetic polymorphism,
- MeSH
- alely MeSH
- B-lymfocyty imunologie metabolismus MeSH
- dospělí MeSH
- exprese genu * MeSH
- frekvence genu MeSH
- haplotypy MeSH
- HLA-DQ alfa řetězec genetika MeSH
- HLA-DQ beta řetězec genetika MeSH
- lidé středního věku MeSH
- lidé MeSH
- messenger RNA genetika MeSH
- mladý dospělý MeSH
- monocyty imunologie metabolismus MeSH
- orgánová specificita MeSH
- senioři MeSH
- zdraví dobrovolníci pro lékařské studie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- HLA-DQ alfa řetězec MeSH
- HLA-DQ beta řetězec MeSH
- HLA-DQA1 antigen MeSH Prohlížeč
- HLA-DQB1 antigen MeSH Prohlížeč
- messenger RNA MeSH
The submitted paper deals with possibilities of assessment of human age based on findings of stereospecific breakdown of proteins containing D-forms of aspartic acid. The stereospecificity of enzymatic breakdown assumes that after enzymatic hydrolysis peptide breakdown products with different molecular weights--the so-called peptide map--will be formed, depending how many D-aspartyl residues the protein contains. The authors proved in the submitted preliminary study in subjects of different age the formation of breakdown products of different size in non-collagenous proteins of human dentin which was hydrolyzed by protease V8 from Staphylococcus aureus.
- MeSH
- dentin chemie MeSH
- dospělí MeSH
- fosfoproteiny analýza MeSH
- lidé MeSH
- peptidové mapování * MeSH
- senioři MeSH
- určení zubního věku metody MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- senioři MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- fosfoproteiny MeSH
- phosphophoryn MeSH Prohlížeč
