Recent experimental observations have shown that the thyroid gland plays a dominant part in the induction and maintenance of anoestrus in ewes. The mechanisms of the anoestrous effects of the thyroid gland are still unclear. On the basis of experiments, in which after thyroidectomy at the onset of sexual activity LH production was maintained also during the spring months, iodothyronines have been supposed to stimulate the inhibitory effects of oestrogens upon the neuroendocrine centres that generate pulsatile LH secretion (Moenter et al., 1991; Webster et al; 1991). However, in our previous work (Bekeová et al., 1995) we observed significant changes in iodothyronine levels, mainly T3, in ewes treated with FSH, LH-RH and oxytocin-based preparations in 24 and 72 h after parturition in the spring. Having made the above observations we suppose seasonal anoestrus to result rather from changes in thyroid and ovarian hormone interactions or from a decrease in thyroid hormone levels that is induced by a temporary decrease in sexual hormones in this phase of the year. Within investigations into the effects of thyroid hormones and their interactions in spring this study focused on the response of the thyroid gland and ovaries in anoestrous ewes to chlorsuperlutin and PMSG treatment in the second half of May. Eighteen Slovak Merino ewes were divided into an experimental and a control group counting 15 and 3 animals, respectively. The experimental animals were each treated with 20 mg chlorsuperlutin (Agelin Spofa vaginal inserts) for 12 days. On day 12 the inserts were removed and each animal was given 500 IU PMSG. In the same time intervals the controls were treated with a placebo (sterile polyurethane, saline). Blood samples were obtained prior to swab insertion (day 0) and in 4-day intervals under chlorsuperlutin treatment (days 4, 8 and 12). For the first 24 h after PMSG-treatment blood samples were taken in 2-hour intervals and then in 48 and 72 h. For radioimmunological determination of T4, T3, E2 and P4 levels the RIA-test-T4, RIA-test-T3, RIA-test-Estra and RIA-test-Prog commercial kits (manufacturer: URVJT Kosice, Slovak Republic) were used, respectively. When compared to the almost constant but significantly lower T4 values in the controls (P < 0.05; P < 0.01; Tab. II, Fig. 1), a repeated massive release of T4 occurred in the experimental animals (Tab. I, Fig. 1). Its first peak observed 4 h after PMSG was significant in comparison both to Day 0 and the controls (P < 0.05 and P < 0.01, respectively). The same was true for the 2nd peak observed 20 h after PMSG-treatment (P < 0.001 and P < 0.01, respectively). The dynamics of T3 was similar in both groups. The transitory increase in T3 levels observed in the controls (Tab. II, Fig. 2) on day 4 of chlorsuperlutin treatment was insignificant when compared to day 0. Both the decrease observed between day 8 and of chlorsuperlutin treatment and 20 h after PMSG gavage, and the increase between 24 and 72 h appeared to be insignificant. Comparison to day 0 revealed increased T3 levels in the experimental group (Tab. I, Fig. 2) on days 4 and 8 of chlorsuperlutin treatment, the levels of significance being P < 0.01 and 0.05, respectively. Between 8 and 24 h after PMSG-gavage, in contrast to the controls, T3 levels in the experimental animals acquired the character of a slowly increasing rhythmic pulsation. At 72 h after PMSG a significant decrease occurred (P < 0.05). In the control animals (Tab. II, Fig. 3) E2 levels revealed interchanging episodes of insignificant increase and decrease beneath test sensitivity. In the experimental ewes (Tab. I, Fig. 3) a double-peaked elevation of E2 could be observed, the first (insignificant) peak occurring 18 and 20 h and the second (significant) one 48 and 72 h following PMSG treatment (P < 0.05 and 0.01, respectively). The inter-group differences were significant at the level of P < 0.05 in each case.

• MeSH
• chlormadinon analogy a deriváty   farmakologie   MeSH
• estradiol krev   MeSH
• gonadotropiny koňské farmakologie   MeSH
• hormony štítné žlázy krev   MeSH
• kongenery progesteronu farmakologie   MeSH
• ovce krev   MeSH
• progesteron krev   MeSH
• roční období MeSH
• thyroxin krev   MeSH
• trijodthyronin krev   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• chlormadinon MeSH
• chlorsuperlutin MeSH Prohlížeč
• estradiol MeSH
• gonadotropiny koňské MeSH
• hormony štítné žlázy MeSH
• kongenery progesteronu MeSH
• progesteron MeSH
• thyroxin MeSH
• trijodthyronin MeSH

Observation of hematological indices which are a reflection of physiological processes in the individual phases of the reproductive cycle, presents one of the methods of controlling the homeostasis of the ewe's organism. This work focused on the observation of selected indices during synchronization treatment and in the first days of pregnancy The experiment included 10 conventionally bred animals. Blood was obtained from the v. jugularis into an oxalate mixture on days 0 (date of synchronization), 3, 7 and 14 of Agelin implantation, on the day of oestrus and days 7, 14, 17 and 34 of pregnancy. Erythrocyte counts were determined by the Bürker flask method, hemoglobin concentrations by spectrophotometry and packed cell volume (PCV) by the method of Janetzki. For leucocyte count determination the Bürker flask method with Turk's solution was used; the percentual proportion of leucocytes was stated by the leucogram. During synchronization treatment and pregnancy the mean erythrocyte counts ranged from 9.16 +/- 0.81 to 9.74 +/- 0.61 T/l and from 9.09 +/- 0.84 to 9.47 +/- 1.11 T/l, respectively. Hemoglobin concentrations oscillated between 10.71 +/- 1.02 and 11.83 +/- 1.01 g/l. PCV values reached between 32.44 +/- 2.18 and 39.33 +/- 2.73 l/l with a significant increase on day 14 of Agelin implantation and days 17 and 34 of pregnancy (P < 0.05). This dynamics of the red blood components points to the occurrence of functional-morphological changes in the sexual apparatus during oestrus and Agelin treatment. Through mediation by the vascular system a change in the mean values may also occur (Kresan et al., 1979).(ABSTRACT TRUNCATED AT 250 WORDS)

• MeSH
• hematokrit veterinární   MeSH
• hemoglobiny analýza   MeSH
• krevní obraz veterinární   MeSH
• ovce krev   MeSH
• synchronizace říje * MeSH
• těhotenství u zvířat krev   MeSH
• těhotenství MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• hemoglobiny MeSH

The active transport of mineral substances by cell membranes plays an important role in the neurohumoral regulation of reproductive processes in farm animals. A great importance is attributed to lipoproteins as sources of the primary precursor of steroid synthesis in the placenta and ovaries (Knopp et al., 1986; Grummer et al., 1988). The effects of hormones on K metabolism in the blood serum of ewes were studied by Hawk et al. (1961). Krajnicáková et al. (1993) observed the dynamic changes of electrolytes in the course of the reproductive cycle in ewes. Na and K levels and their relation to ovarian hormones were determined in the systemic blood stream of ewes during oestrus synchronization and pregnancy. Ten Slovak Merino ewes aged 3-5 years and weighing 40-50 kg were included in the experiment. The animals were reared conventionally. A feed ration consisted of silage, hay, feeding beet-pulp, straw and in the last two months of pregnancy it was supplemented with roughage. Water and salt were given ad libitum. Blood samples were obtained from the jugular vein prior to synchronization (day 0) and on days 3, 7 and 14 of synchronization with Agelin vaginal implants. After removing the implants the animals received 500 I.U. PMSG (Pregnant Mare Serum Gonadotropin). The animal in oestrus cycle were inseminated with fresh diluted semen. Reinsemination was not performed in this experiment. Sampling was continued on the day of insemination and on days 7, 14 and in month 2, 3, 4 and 5 of pregnancy. Na and K levels were measured by atomic absorbtion spectrophotometry using the Atom Spek device (RANG-HIGLER). Progesterone (P4) and 17 beta-oestradiol (E2) levels were assayed radioimmunologically using the respective kits (RIA-test-PROG and RIA-test-ESTRA) manufactured by URVJ Kosice. During the observation period Na levels fluctuated within 131.50 +/- 1.71 and 153.55 +/- 4.77 nmol/l of serum (Tab. I) and significantly decreased between day 14 of synchronization and the end of the investigation (P < 0.05; P < 0.01; P < 0.001). Potassium levels were observed to significantly decrease from the day of insemination on, throughout the first days of pregnancy and the above-mentioned months of gravidity, the values ranging between 4.57 +/- 0.52 and 3.93 +/- 0.36 nmol/l.(ABSTRACT TRUNCATED AT 400 WORDS)

• MeSH
• draslík krev   MeSH
• estradiol krev   MeSH
• ovce krev   MeSH
• progesteron krev   MeSH
• sodík krev   MeSH
• synchronizace říje krev   MeSH
• těhotenství u zvířat krev   MeSH
• těhotenství MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• draslík MeSH
• estradiol MeSH
• progesteron MeSH
• sodík MeSH

Triacylglycerols as the sources of cholesterol, the primary precursor of steroid synthesis, are one of the factors that limit the speed of steroidogenesis. Synthesis and resynthesis of triacylglycerols depend, in addition to other factors, on phosphatid hydrolysis that is controlled during pregnancy by placentary ALP (Sumikawa et al., 1987) and the FFA pool in the systemic blood stream. Enzymes participating in FFA release from the fat tissue are affected by thyroid hormones and TSH (Mayes, 1977). In view of the above facts, this work was aimed at the observation of changes in alkaline phosphatase (ALP), thyroxine (T4), triiodothyronine (T3), 17 beta-oestradiol (E2) and progesterone (P4) levels and their mutual correlations in the systemic blood stream of sheep from the first day after parturition to day 51 p.p. Our attention was paid to the possible delayed effects of placentary ALP and thyroid hormones on the synthesis of ovarian steroids in puerperal ewes. Nine merino sheep were included in the experiment; they lambed in the first decade of February and were kept together with their lambs in a classical sheep-cot on deep litter. The animals were fed according to the Czechoslovak State Standard CSN 46 7070. Blood samples were taken from the v. jugularis from 8 to 9 o'clock a.m. 24 hs (day -1) prior to parturition (a.p.), 36 hs and on days 4, 7, 14, 17, 21, 25, 34, 42 and 51 after parturition (p.p.). As compared with day -1 (2.6 +/- 1.08 mu kat/l), ALP concentrations showed a decrease with minimum values occurring on day 17 (1.34 +/- 0.97 mu kat/l, P < 0.05). T4 concentrations also decreased from day -1 (59.4 +/- 9.69 nmol/l) to day 21 (54.89 +/- 11.06 nmol/l, P < 0.05). A similar decrease (from 1.82 +/- 0.33 nmol/l on day 1 to 0.85 +/- 0.32 nmol/l on day 21) could also be observed in T3 levels. As early as 36 hs p.p., the concentrations of both steroid hormones, E2 and P4, significantly decreased [from 2.45 +/- 0.65 and 9.46 +/- 0.34 nmol/l on day -1 to 0.30 +/- 0.24 and 0.05 +/- 0.08 nmol/l, respectively (P < 0.001)]. Throughout the period of investigation, apart from an unsubstantial increase in E2 values on days 17 and 42 and an episodic increase in P4 levels on day 34, the concentrations of both steroids had the values that were significantly lower than those observed on day -1.(ABSTRACT TRUNCATED AT 400 WORDS)

• MeSH
• alkalická fosfatasa krev   MeSH
• estradiol krev   MeSH
• hormony štítné žlázy krev   MeSH
• ovce krev   MeSH
• poporodní období krev   MeSH
• progesteron krev   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• alkalická fosfatasa MeSH
• estradiol MeSH
• hormony štítné žlázy MeSH
• progesteron MeSH

Our investigations were concerned with dynamic changes in total lipids (CL), cholesterol (CHOL) and progesterone (P4) in blood serum of sheep in the period of oestrus synchronization treatment and during mating and gravidity. Our experiment was carried out using 10 animals housed under the conditions of productive rearing. Blood samples were taken from v. jugularis on day of swab application (day 0) and on days 3 and 7 of the action of Agelin vaginal swabs, on day of insemination, and on days 7, 14, 17 and in the 2nd, 3rd and 4th month of gravidity. Blood serum was used to determine total lipids and cholesterol by means of Bio-Lachema tests, and P4 concentrations employing RIA-test-Prog kits (URVJT, Kosice). A statistically significant decrease in concentrations of total lipids (Fig. 1, Tab. I) in sheep blood serum was recorded on day of insemination (P < 0.05) compared to day 0, with the value 1.59 +/- 0.31 g/l of serum, and in the 3rd month of gravidity (P < 0.01), at concentrations 1.36 +/- 0.38 g/l of serum. The determined decrease in their values in the mentioned period can be modulated by the mutually changing ratio of steroid hormones or by inhibition of synthesis of lipoproteins responsible for changes in total plasma lipids. Changes in cholesterol concentrations (Fig. 2, Tab. I) during the introduction of swabs were insignificant and ranged from 1.60 +/- 0.42 to 1.73 +/- 0.33 mmol/l of serum. An insignificant increase in cholesterol concentrations (P < 0.05), with its highest levels 1.98 +/- 0.43 mmol/l of serum, was recorded in the 3rd month of gravidity.(ABSTRACT TRUNCATED AT 250 WORDS)

• MeSH
• cholesterol krev   MeSH
• lipidy krev   MeSH
• ovce krev   MeSH
• progesteron krev   MeSH
• synchronizace říje krev   MeSH
• těhotenství u zvířat krev   MeSH
• těhotenství MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• cholesterol MeSH
• lipidy MeSH
• progesteron MeSH

A postparturient period is characterized by low basal secretion of adenohypophysis gonadotropins with the following appropriate changes in ovarian hormones and their response to the morphology of vaginal epithelium. In this study the dynamics of the cytological picture of vaginal swabs and ovarian hormones 17 beta-oestradiol (E2) and progesterone was investigated in the puerpery of ewes. The objective was to obtain and extend the knowledge of cytological changes in vaginal epithelium and levels of ovarian hormones of ewes after parturition and of their relationships from the first several days after lambing until the 51st day of the period of observation. Vaginal swabs for vaginal cytology were taken from nine ewes on days 1, 4, 7, 14, 17, 21, 25, 34, 42 and 51 after parturition. These swabs were fixed in ether-alcohol 1:1, stained according to the Faltínová-Zidovský method, embedded in Canada balsam and evaluated by differentiation of cells according to Luksh (1953). Blood samples for E2 and P4 determinations were taken from the jugular vein in the same intervals as vaginal swabs. The serum was centrifuged and stored at -18 degrees C until use. E2 and P4 concentrations were determined radioimmunologically, using kits RIA-test ESTRA and RIA-test PROG from URVJT Kosice. A statistically significant decline (P < 0.05) of percentual representation of basal and parabasal cells (Fig. 1, Tab. I) on day 7 after lambing was replaced by their multiplication from day 14 reaching the values of 66.07 +/- 3.95 on day 42. A statistically significant decrease in intermediary flat cells (Fig. 2, Tab. II) was observed on days 14 (P < 0.001), 34 and 42 (P < 0.01; P < 0.001), in comparison with the first day after lambing. An evaluation of intermediary convoluted cells revealed their highest percentage on days 1 and 17 after parturition (34.65 +/- 4.77-20.62 +/- 12.57) and their decline to values in the range of 6.77 +/- 1.46-7.66 +/- 2.25 on the remaining days of the period of observation. Percent occurrence of superficial flat cells (Fig. 3, Tab. I) ranged from 3.9 +/- 1.10 to 10.63 +/- 7.23 from day 1 to day 51 after lambing. The lowest percentual representation (1.32 +/- 0.79-4.10 +/- 1.89) was recorded for superficial convoluted cells. Multiplication of the evaluated cells was observed, reaching the highest but insignificant representation (P > 0.05) on day 25 of postparturient investigation: 4.10 +/- 1.89 (Fig. 3, Tab. I). 17 beta-oestradiol (E2) concentrations were compared to the -1st day before parturition, when its values varied at the level of 2.45 +/- 0.64 nmol/l serum.(ABSTRACT TRUNCATED AT 400 WORDS)

• MeSH
• estradiol krev   MeSH
• ovce anatomie a histologie   krev   MeSH
• poporodní období * MeSH
• progesteron krev   MeSH
• vagina cytologie   MeSH
• vaginální stěr veterinární   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• estradiol MeSH
• progesteron MeSH

Application of new procedures in the sphere of the control of sexual functions requires an extension of present knowledge of postparturient endocrinium or endogenic factors comprised in postparturient physiology of sexual activity. According to recent data, oxytocin, besides its uterotonic and luteolytic activity, acts as an ovarian factor in the local intrafollicular regulation of stereidogenesis and as a modulator of uterine secretion of prostaglandines. Based on present knowledge of oxytocin effects, this study was aimed at investigation of the influence of repeated carbetocin (Depotocin inj. Spofa) administration on the dynamics of changes in thyroxine (T4), triiodothyronine (T3), 17 beta-estradiol (E2), progesterone (P4) concentrations and their mutual correlations from the 36th hour till the 51st day after parturition. Simultaneous study of a possible delayed influence of applied carbetocin on conception of ewes after oestrus evocation on day 51 after lambing was carried out. Nineteen ewes of the Slovak Merino breed, lambed in the first decade of February, were assigned to the experimental (n = 9) and to the control group (n = 10). Experimental ewes were subjected to repeated postparturient carbetocin treatment at the dose 0.07 mg per animal. The first dose was applied i. m. in 24 hours, and the second in 72 hours after parturition. On day 51 oestrus was induced in nine ewes of each group by combined treatment with chlorsuperlutin (Agelin, vaginal pessaries, Spofa) and PMSG (500 I.U./animal). On the day of PMSG application ewes were housed together with rams for the period of the next six days. Samples of blood were taken 24 hours before parturition (-1st day), up to 36 h after parturition and on days 4, 7, 14, 17, 21, 25, 34, 42 and 51 after parturition. Concentrations of T4, T3, E2 and P4 were determined by commercial kits RIA-test-T4; RIA-test-T3; RIA-test-ESTRA and RIA-test-PROG (URVJT Kosice). Animal of the control group showed variations of T4 concentrations (Tab. I, Fig. 1) at the level of original values (59.4 +/- 9.69 nmol.l-1) up to the 21st day with the exception of temporary drop on day 4 and rise on day 7, insignificant compared to the -1st day. T4 concentrations of the control group displayed an intermittent increasing trend with the statistically insignificant peak after 36 h and on day 17, compared to the -1st day. After the 21st day controls revealed a sustained moderate increase while the experimental ewes displayed a decline of its concentrations until the 51st day.(ABSTRACT TRUNCATED AT 400 WORDS)

• MeSH
• estradiol krev   MeSH
• fertilizace účinky léků   MeSH
• hormony krev   MeSH
• léky s prodlouženým účinkem MeSH
• ovce krev   MeSH
• oxytocin aplikace a dávkování   analogy a deriváty   farmakologie   MeSH
• poporodní období * MeSH
• progesteron krev   MeSH
• těhotenství MeSH
• thyroxin krev   MeSH
• trijodthyronin krev   MeSH
• uterotonika aplikace a dávkování   farmakologie   MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• carbetocin MeSH Prohlížeč
• estradiol MeSH
• hormony MeSH
• léky s prodlouženým účinkem MeSH
• oxytocin MeSH
• progesteron MeSH
• thyroxin MeSH
• trijodthyronin MeSH
• uterotonika MeSH

Postparturient anoestry, spontaneously changing over to seasonal anoestry, represents an important reserve of full utilization of the reproductive potential of sheep. In spite of the fact that the length of gestation in sheep only amounts to 148 days (+/- 5 days), inability of most sheep to enter the cycle during the spring season acts as a factor limiting the number of lambings--and at the same time production of lambs--to one lambing per year. In order to obtain more detailed knowledge of hormonal conditions in sheep puerpery, the presented work was directed at the study of dynamic changes in concentrations of thyroxine (T4), triiodothyronine (T3), 17 beta-estradiol (E2), progesterone (P4) and cholesterol (Chol) in the blood serum of ewes and at their mutual correlative dependences between the 36th hour and the 51st postparturient day. The observation was carried out in nine nursing ewes of the Slovak Merino breed, with average weight 40-50 kg, lambed in January and February. Blood was sampled by means of jugular vein puncture 14 days (-14th day) before parturition (a. p.), up to 36 h after parturition (p. p.) and on day 4, 7, 14, 17, 21, 25, 34, 42 and 51 p. p. In the period from the 36th h to day 21 p. p., concentrations of T4 (Tab. I, Fig. 1) showed a decreasing tendency compared to the starting -14th day (69.55 +/- +/- 0.12 nmol.-1) with the exception of a temporary increase on the 7th day p. p. The lowest values were recorded on days 4 and 21 p. p. (45.66 +/- 21.61 nmol.l-1, P less than 0.05 and 54.89 +/- 11.06 nmol.l-1, P less than 0.05, resp.). An increase of these values to the starting level was observed between days 25 and 51 p. p. Compared to the values on -14th day (0.76 +/- 0.22 nmol.l-1), a significant increase of T3 concentrations (Tab. I, Fig. 2) was recorded at the 36th h and on days 4 and 7 p. p. with the highest values (1.48 +/- +/- 0.34 nmol.l-1, P less than 0.001) at 36 h p. p. After the temporary decrease between days 14 and 21 p. p. its concentrations showed a constantly rising tendency statistically significant on days 34, 42 and 51 p. p., compared to the -14th day (P less than 0.05). Concentrations of E2 (Tab. I, Fig. 3) reached the values of 0.44 +/- 0.41 nmol.l-1 on the -14th day.(ABSTRACT TRUNCATED AT 400 WORDS)

• MeSH
• cholesterol krev   MeSH
• estradiol krev   MeSH
• hormony štítné žlázy krev   MeSH
• hormony krev   MeSH
• ovce krev   MeSH
• poporodní období krev   MeSH
• progesteron krev   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• cholesterol MeSH
• estradiol MeSH
• hormony štítné žlázy MeSH
• hormony MeSH
• progesteron MeSH

Objectivisation of the physiological course of puerpery and control of involution processes of uterus represent topical questions of an increased frequency of lambing. Our study was directed at the determination of the weight, length of body and horns of uterus as well as of the weight, length, width and height of ovaries, following the administration of carbetocin (Depotocin inj. Spofa), in the puerperal period of Slovak Merino sheep. The study was aimed at obtaining objective biometric data on uterus and ovaries and recording the influence of carbetocin on the involution of uterus and ovaries of sheep. Thirty ewes, divided into two groups, were included in the experiment. The animals of the experimental group (n = 15) were subjected to treatment with carbetocin (Depotocin inj. Spofa) at the amount 2 x 0.07 mg. The first dose was applied intramuscularly (i. m.) on the 2nd day after parturition and the second dose was injected subcutaneously (s.c.) on the 4th day after lambing. The second group (n = 15) served as a control. Three ewes (n = 3) were bled on each of the following days: 1, 7, 17, 25 and 34 after parturition. After bleeding the sexual organs were removed by excision and their weight and the length of the body and horns of uterus and of ovaries was determined. The number and size of follicles were determined on the surface of ovaries. The weight of uterus body showed a declining tendency in sheep of the control group with a statistically significant decrease from the 17th till the 34th day after parturition (P less than 0.01). Its weight varied from 56.0-14.6 to 5.2-0.3 g in the above-mentioned time interval. The experimental group showed a statistically significant decrease in weight as early as on the 7th day after lambing compared to the 1st day after parturition, down to the values 185.66-38.91 g (P less than 0.05). The decrease in the weight of uterus body recorded on day 17 (106.56-41.08 g) was less pronounced than in the control group. The final average weight of the body of uterus on the 34th day after parturition was 41.36-8.52 g, that means higher than in the control group (5.2-0.3 g). The weight of gravid horns in the experimental group declined significantly as early as on the 7th day after parturition to the average weight of 60.89-9.45 g and reached 9.86-3.49 g on the 34th day.(ABSTRACT TRUNCATED AT 400 WORDS)

• MeSH
• biometrie MeSH
• ovarium anatomie a histologie   účinky léků   MeSH
• ovce anatomie a histologie   MeSH
• oxytocin analogy a deriváty   farmakologie   MeSH
• poporodní období * MeSH
• uterotonika farmakologie   MeSH
• uterus anatomie a histologie   účinky léků   MeSH
• zvířata MeSH
• Check Tag
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• carbetocin MeSH Prohlížeč
• oxytocin MeSH
• uterotonika MeSH

Knowledge of pathogenesis of sexual dysfunctions at altered thyroid activity is limited by the knowledge of multiple and ubiquitous action of its hormones throughout the organism. One of the possibilities of modulatory influence of thyroid hormones on sexual functions can be realized through the participation of thyroxine and triiodothyronine in the synthesis and metabolism of primary substrate of steroid synthesis--cholesterol. The presented work is aimed at the study of simultaneous dynamic changes of concentrations of thyroxine (T4), triiodothyronine (T3), 17 beta-estradiol (E2), progesterone (P4) and cholesterol (Chol) during synchronization of the rutting period and gravidity at parallel correlative evaluation of mutual relations of the followed parameters in ten Merino sheep in the seasonal period. Synchronization was achieved by chlorsuperlutin (Agelin--vaginal swabs, Spofa; 20 mg of chlorsuperlutin/swab) and PMSG (500 I. U./animal). Blood was sampled by means of a jugular vein puncture at the time of swab insertion (-13th day) and after three (-10th day) and seven (-7th day) following days, at the removal of swabs and application of PMSG (-3rd day), on the day of insemination (zero day), on the 7th, 14th and 17th day and in the middle of the 2nd, 3rd, 4th and 5th month of gravidity. In the phase of oestrus synchronization a significant increase of E2 concentrations on days -7 and -3 of the experiment (0.47 +/- 0.079 and 0.542 +/- 0.177 nmol.l-1 of serum, P less than 0.001; P less than 0.001) was observed compared to the E2 values on day -13 (0.084 +/- 0.036 nmol.l-1 of serum). Parallel to these observations, marked intermittent changes of T4 (Tab. I, Graph 1) were recorded with the lowest values of this parameter observed on days -10 (41.75 +/- 20.23, P less than 0.05) and -3 (50.22 +/- 18.77, P less than 0.05) and the highest on day -7 (96.77 +/- 17.51 nmol.l-1, P less than 0.01) and day zero (85.40 +/- 19.59 nmol.l-1 of serum, P less than 0.05) in comparison with the -13th day (67.22 +/- 18.29 nmol.l-1 of serum). Concentrations of P4 (Tab. I, Graph 4) declined to the lowest values on day zero observation (0.09 +/- 0.08 nmol.l-1 of serum, P less than 0.05 vs 3.40 +/- 3.61 nmol.l-1 on day -13). No significant changes of concentrations of T3 (Tab. I, Graph 2) and Chol (Tab. I, Graph 5) were observed during oestrus synchronization. During gravidity, concentrations of E2 (Tab. I Graph 3) showed an increasing trend compared to the -13th day.(ABSTRACT TRUNCATED AT 250 WORDS)

• MeSH
• chlormadinon analogy a deriváty   farmakologie   MeSH
• cholesterol krev   MeSH
• estradiol krev   MeSH
• hormony krev   MeSH
• ovce fyziologie   MeSH
• progesteron krev   MeSH
• synchronizace říje krev   účinky léků   MeSH
• těhotenství u zvířat krev   účinky léků   MeSH
• těhotenství MeSH
• thyroxin krev   MeSH
• trijodthyronin krev   MeSH
• zvířata MeSH
• Check Tag
• těhotenství MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• chlormadinon MeSH
• chlorsuperlutin MeSH Prohlížeč
• cholesterol MeSH
• estradiol MeSH
• hormony MeSH
• progesteron MeSH
• thyroxin MeSH
• trijodthyronin MeSH