West Nile virus (WNV) is a mosquito-borne zoonotic neurotropic virus capable to cause lethal meningoencephalitis (WNE) in infected hosts such as birds, horses, and humans. Due to their sensitivity, horses serve as sentinel species in areas at risk. We studied a population of Camargue horses living in Southern France in two zones with endemic WNV circulation where WNV outbreaks were recorded in 2000 and 2003-4. Two sets of microsatellite markers located in MHC and Ly49 genomic regions were genotyped as well as multiple SNPs in ten immunity-related candidate gene regions. Associations between genetic polymorphisms and resistance/susceptibility to WNE were tested. While single marker associations were weak, compound two-gene genotypes of SNPs located within the MAVS, NCR2 and IL-10 genes and microsatellites HMS082 and CZM013 were associated with susceptibility to WNE. Combinations of microsatellite markers CZM009, ABGe17402 and ABGe9019 were associated with simple seroconversion without clinical signs of WNE (resistance). In addition, a distribution of polymorphic markers between WNV-IgG seropositive horses and a control group of WNV-IgG seronegative horses was tested. One SNP in the OAS1 gene (NC_009151.3:g.21961328A>G) was significantly associated with the seropositive phenotype (pcorr = 0.023; OR = 40.5 CI (4.28; 383.26); RR = 8.18 CI (1.27; 52.89) in the Camargue breed. In compound genotypes, SNP markers for SLC11A1, MAVS, OAS1, TLR4, ADAM17 and NCR2 genes and ten microsatellites showed non-random distribution between seropositive and seronegative groups of horses. Further analysis of associated markers could contribute to our understanding of anti-WNV defense mechanisms in horses.

• Klíčová slova
• Associations, Camargue, Horse, Immunity-related candidate gene SNP markers, MHC and Ly49 microsatellite markers, West Nile virus,
• MeSH
• genetická predispozice k nemoci genetika   MeSH
• genetické markery genetika   MeSH
• imunoglobulin G imunologie   MeSH
• jednonukleotidový polymorfismus MeSH
• koně MeSH
• mikrosatelitní repetice MeSH
• nemoci koní genetika   virologie   MeSH
• odolnost vůči nemocem genetika   MeSH
• polymorfismus genetický * MeSH
• virus západního Nilu fyziologie   MeSH
• západonilská horečka genetika   veterinární   virologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Francie epidemiologie   MeSH
• Názvy látek
• genetické markery MeSH
• imunoglobulin G MeSH