The effect is described of selenium supplemented in an inorganic and organic form on the innate immune response of goats. Though the phagocytic activity (as a marker of the immune function) was found to be lower in organic-Se-treated group than in control (54.5 +/- 4.32 vs. 60.2 +/- 9.15 %), it did not generally exhibit any significant differences; similarly, no differences were found in the phagocytic index. The production of reactive oxygen species (ROS) was determined using the luminol-enhanced chemiluminescence (CL) (estimated as peak CL, integral CL and a peak time after addition of calcium ionophore A23187, opsonised zymosan (OZP) and phorbol-12-myristate-13-acetate as effectors. A significant ROS increase reflected in integral CL and a peak time was found in the inorganic-Se-treated group when OZP was used as activator; other parameters did not exhibit significant changes. The supplementation of Se in inorganic form can thus be seen to influence positively the innate immune system of kids.

• MeSH
• anorganické látky aplikace a dávkování   MeSH
• fagocytóza účinky léků   MeSH
• imunologické faktory aplikace a dávkování   MeSH
• kozy MeSH
• organické látky aplikace a dávkování   MeSH
• přirozená imunita účinky léků   MeSH
• reaktivní formy kyslíku metabolismus   MeSH
• selen aplikace a dávkování   MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• anorganické látky MeSH
• imunologické faktory MeSH
• organické látky MeSH
• reaktivní formy kyslíku MeSH
• selen MeSH

alpha-Glucosidase from two microbial sources, Bacillus stearothermophilus and Brewer's yeast, has been used to catalyze transglycosylation reactions and a comparative study was carried out to determine the regioselectivity of this reaction. Bacterial alpha-glucosidase exhibited higher transfer activity with maltose and was able to synthesize tri- and tetrasaccharides in high yield (27%). In the case of yeast enzyme, only trisaccharides were synthesized in lower yield. Structure analysis of transglycosylation products by means of GC-MS and NMR spectroscopy revealed a correlation between the hydrolytic substrate specificity and the regioselectivity of transglycosylation reaction. Higher substrate specificity of bacterial enzyme, however, influenced its transglucosylation activity toward other saccharide acceptors.

• MeSH
• alfa-glukosidasy metabolismus   MeSH
• glykosylace MeSH
• hydrolýza MeSH
• magnetická rezonanční spektroskopie MeSH
• oligosacharidy chemická syntéza   MeSH
• plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
• stereoizomerie MeSH
• substrátová specifita MeSH
• vysokoúčinná kapalinová chromatografie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alfa-glukosidasy MeSH
• oligosacharidy MeSH