JavaScript NENÍ povolen !

Prosím povolte JavaScript.

* Zobrazit nápovědu

Reset

Autor: Matousová, O

27 záznamů v PubMed Filtry

Článek

Densitometrické stanovení dithiadenoxidu v krevní plazmĕ
[Densitometric determination of dithiadenoxide in blood]

Ceskoslovenska farmacie. 1993 Apr ; 42 (2) : 86-7.

Cesk Farm
ISSN 0009-0530
Zdroj

The present paper describes a method of dithiadenoxide determination in blood plasma. Dithiadenoxide was reduced to dithiaden with zinc dust in acidified plasma. The solution was alkalified and dithiaden extracted into n-hexane and isolated on a thin layer of silica gel using the system chloroform--n-heptane-methanol (60:22:18). After spraying the plate with a solution of m-nitrophenyldiazoniumfluoroborate in acid medium, dithiaden stains were evaluated densitometrically in the absorption maximum of the reaction product at 565 nm (RF = 0.37). Detection limit of dithiadenoxide in the proposed method is 0.004 microgram in an applied amount.

Článek

Poruchy hemostázy u monoklonálních gamapatií
[Hemostasis disorders in monoclonal gammopathies]

Sbornik lekarsky. 1991 Oct ; 93 (9-10) : 277-86.

Sb Lek
ISSN 0036-5327
Zdroj

The authors investigated haemostatic parameters of 43 patients with monoclonal gammapathy with principal diagnosis of multiple myeloma (MM-35), non-Hodgkin's lymphoma (NHL-3), M. Waldenström (3) and monoclonal gammapathy of undetermined significance (MGUS-2). Primary haemostasis defect was found in 24 patients. With the exception of 2 thrombocytopenic patients, the defect of aggregation, procoagulant activity and retraction is supposed to be caused by paraprotein. The examination of the 14C 5-HT release and TX B2 synthesis in thrombocytes showed, that platelet activation is unaffected and the paraprotein interferes with interactions of thrombocytes or with coagulation system. In plasma coagulation system explicit abnormalities were found only in thrombin time in 12 patients. The more detailed examination disclosed, that the defect resulted from paraprotein interference with fibrin monomer polymerisation. The thrombin proteolytic activity remained unaffected. In 3 patients shortened euglobulin lysis time was observed. Laboratory haemostasis defect was found in 26 patients (60%), however, the bleeding symptoms manifested in 5 cases (11%) only. The analysis of study results showed, that the most important abnormalities leading to overt bleeding are thrombocytopenia or combined haemostasis defect. Isolated laboratory defects remained silent in most cases.

Článek

Densitometrické stanovení piroxicamu v plazmĕ
[Densitometric determination of piroxicam in plasma]

Ceskoslovenska farmacie. 1991 Mar ; 40 (1) : 40-1.

Cesk Farm
ISSN 0009-0530
Zdroj

Piroxicam was extracted from acid medium into chloroform and isolated on a thin layer of silica gel with the use of the system chloroform - absolute ethanol - benzene - aqueous ammonia (20:15:15:0.5). The intensity of fluorescence of stains was measured densitometrically (an Hg lamp, a filter FL - 39, 330 nm). The limit of detection of the stain is 0.020 microgram/ml of plasma.

Článek

Glanzmannova trombastenie--defekt povrchové membrány krevních desticek
[Glanzmann thrombasthenia--a defect in the surface membrane of platelets]

Vnitrni lekarstvi. 1990 Aug ; 36 (8) : 729-37.

Vnitr Lek
ISSN 0042-773X
Zdroj

The authors present a clinical description, detailed platelet function analysis, and certain biochemical parameters in two siblings with Glanzmann thrombasthenia (G. t.). The isolated occurrence of this disorder in the family corresponds with its autosomal recessive inheritance. In both cases blood platelets completely failed to aggregate. In contrast, the platelet interaction with ristocetin, reflecting their ability to adhere to the subendothelium, the so-called "shape change", the storage granule contents and their release and arachidonic acid metabolism were unaffected. Further, the aggregation abnormality was accompanied by marked procoagulant activity and clot retraction defects; these functions, similarly as aggregation, are implemented on the platelet surface. The analysis of blood platelet proteins, using two dimensional polyacrylamide electrophoresis, confirmed the absence of glycoprotein GP IIb and IIIa and a decrease of the fibrinogen content. The analysis of these findings in G. t. led to the contemporary concept that GP IIb and IIIa on the platelet surface act as receptors for platelet aggregation.